FastQCFastQC Report
Thu 28 Sept 2023
SRR1062558.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062558.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG927542.3188500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTGAGATCGGAAGAGCACACGTCTG333310.833275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG252070.630175TruSeq Adapter, Index 11 (100% over 49bp)
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC243490.608725No Hit
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA242290.6057250000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAACAGATCGGAAGAGCACACGTCTG234410.586025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG213420.53355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC209830.524575No Hit
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA189730.474325Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG181420.45354999999999995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA180290.450725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG152120.38029999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA148070.37017500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC146890.367225No Hit
AGGGAGTCGGGTTCAGATCCCCGAACCCGGAGAGATCGGAAGAGCACACG144530.361325No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAACGAGATCGGAAGAGCACACGTCT123240.3081Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT122330.305825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAAAGATCGGAAGAGCACACGTCTGA121450.303625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG115910.289775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT115560.2889Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGT115450.288625No Hit
GCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA112880.2822Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGAA111890.279725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT102710.25677500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCGGGGACGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC97570.243925Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTGAA93150.232875Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTGA91110.22777499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCTAGATAACCTCGGGCCGATCGCGCAGATCGGAAGAGCACACGTCTG86400.216Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT85120.21280000000000002No Hit
AACTCTTAGCGGTGGATCACTCGGAGATCGGAAGAGCACACGTCTGAACT84550.211375Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCTGGTTGATCCTGCCAGTAACAGATCGGAAGAGCACACGTCTGAACT83120.20779999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTG82180.20545Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAACGGCAGATCGGAAGAGCACACGT81690.204225No Hit
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT79430.198575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAAGATCGGAAGAGCACACGTCTGAA79170.19792500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTCTAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTCTGAAC77960.1949Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC75580.18894999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCCCCTCGCCCCGACTTCGCCGCTTGAGATCGGAAGAGCACACGTCTG71940.17985Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTAGATCGGAAGAGCACACGTCTGA71250.178125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG70740.17685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTGA70240.1756Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT70090.175225Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGACCTCAGATCAGACGAGACAACCCGCTAGATCGGAAGAGCACACGTC69530.173825No Hit
ACGACCTCAGATCAGACGAGACAACCCGCAGATCGGAAGAGCACACGTCT68990.172475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA68470.171175Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT67750.169375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGA67380.16845Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGA67240.1681Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGTCGGGTTCAGATCCCCGAACCCGGAGAGATCGGAAGAGCACACGT66430.166075No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG62950.15737500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT62220.15555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG60810.152025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGT60540.15135No Hit
TGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGAAC60000.15Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGACCTCAGATCAGACGAGACAACCCGAGATCGGAAGAGCACACGTCTG59960.14989999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC58500.14625No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT56880.14220000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT56290.14072500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGGTGGAGCTAGAGCGCGTGCGATGAGATCGGAAGAGCACACGTCTGA55110.137775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT55100.13775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGAA55050.137625Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGAGATCGGAAGAGCACACGTC54990.137475No Hit
CGCCCCCTCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT54700.13675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTACGGCCATACCACCCTGAGCACGCCCAGATCGGAAGAGCACACGTC54480.1362No Hit
CCCGTGGACGGTGTGAGGCCGGTAACGAGATCGGAAGAGCACACGTCTGA53860.13465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGTGGACGGTGTGAGGCCGGTAACAGATCGGAAGAGCACACGTCTGAA52860.13215Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAAGATCGGAAGAGCACACGTCT52150.130375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGGGCGAGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC52120.1303Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTG51580.12895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC51260.12815000000000001No Hit
ACCCGGGGACGCGTGCATTTATCAAGATCGGAAGAGCACACGTCTGAACT51240.1281Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTCTGAACT50190.125475Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC49790.124475No Hit
GGAGGTGGAGCTAGAGCGCGTGCGATGGAGATCGGAAGAGCACACGTCTG49340.12335Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTG48390.12097500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGGGGCCCGCGCCCTGGAAAGCGCCGCGAGATCGGAAGAGCACACGTC47850.11962500000000001No Hit
CGGCGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC47260.11815No Hit
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG46440.1161Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGA46250.11562499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGGGGCGAGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACT46170.11542499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCGGGGACGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC45880.1147Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTCGATTGGCCTGGGATAGCCGGCCGAGATCGGAAGAGCACACGTCTGA45170.112925Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCT45140.11284999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCGAGAACTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAAC45080.1127Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA44880.1122Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTTCGGGGGCGAAGGTGGCTCGCGGCTAGATCGGAAGAGCACACGTCTG44690.11172499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA44070.11017500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCTTCGATTGGCCTGGGATAGCCGGCCGAGATCGGAAGAGCACACGTC42960.10740000000000001No Hit
TGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTGAA42850.107125Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG42090.105225No Hit
GCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGTCT41240.1031Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT40990.10247500000000001No Hit
GGGTCTAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTCTGAA40950.102375Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCT40750.101875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACTC40720.1018Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG40420.10104999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCAGATCGGAAGAGCACACGTCTG40250.10062499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCT40210.100525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)