FastQCFastQC Report
Thu 28 Sept 2023
SRR1062575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345388
Total Bases178.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG512732.1861201643395463Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTGAGATCGGAAGAGCACACGTCTG175040.7463157481832431Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG141030.6013077580340652TruSeq Adapter, Index 11 (100% over 49bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA134130.5718883186918327Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC125190.5337709581527662No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAACAGATCGGAAGAGCACACGTCTG123520.5266506010945737Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG122880.5239218415034101Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA108790.4638464936291991Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG107040.4563850416221112Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC101890.4344270542869666No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC87530.3732005109602335No Hit
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA83650.3566574059388042Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG80250.34216087061074757Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGAGTCGGGTTCAGATCCCCGAACCCGGAGAGATCGGAAGAGCACACG79750.340029027180151No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA75980.32395492771345297Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA66010.2814459697073576Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAAAGATCGGAAGAGCACACGTCTGA65540.27944203688259683Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT64990.27709700910894064Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAACGAGATCGGAAGAGCACACGTCT64530.2751357131527918Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG61770.2633679374158988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGAA57860.24669692178863367Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT57700.2460147318908428Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT54750.2334368556503231Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTGAA54290.23147555969417424Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGT53440.22785142586216012No Hit
TACCTGGTTGATCCTGCCAGTAACAGATCGGAAGAGCACACGTCTGAACT49500.21105249962905925Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTGA47600.20295149459279233Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTCTAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTCTGAAC46440.1980056178338083Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCTAGATAACCTCGGGCCGATCGCGCAGATCGGAAGAGCACACGTCTG45890.1956605900601521Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGGGGACGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC45680.19476521581930153Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT45380.1934861097609436Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTG45300.19314501481204815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCTTAGCGGTGGATCACTCGGAGATCGGAAGAGCACACGTCTGAACT45260.19297446733760043Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCCGTGGACGGTGTGAGGCCGGTAACGGCAGATCGGAAGAGCACACGT44050.18781540623555676No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAAGATCGGAAGAGCACACGTCTGAA43160.18402072492909488Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT43000.18333853503130398No Hit
TTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTGA43000.18333853503130398Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG41200.17566389868115637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGACCTCAGATCAGACGAGACAACCCGCTAGATCGGAAGAGCACACGTC40260.17165603303163485No Hit
ACGACCTCAGATCAGACGAGACAACCCGCAGATCGGAAGAGCACACGTCT39170.16700861435293435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGA38330.16342711738953214Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC38310.16334184365230828Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGTCGGGTTCAGATCCCCGAACCCGGAGAGATCGGAAGAGCACACGT38240.16304338557202477No Hit
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT37740.1609115421414282Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTAGATCGGAAGAGCACACGTCTGA37380.15937661487139868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT36980.15767114012692143Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT35870.15293844771099707Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA35600.15178725225847492Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG35560.1516167047840272Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGA35410.15097715175484824Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGACCTCAGATCAGACGAGACAACCCGAGATCGGAAGAGCACACGTCTG34540.14726774418561023Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGAAC33860.14436843711999892Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG33160.14138385631716374Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCCCTCGCCCCGACTTCGCCGCTTGAGATCGGAAGAGCACACGTCTG32130.1369922588501348Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTG31760.13541469471149337Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTACGGCCATACCACCCTGAGCACGCCCAGATCGGAAGAGCACACGTC31560.13456195733925475No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT31490.13426349925897121Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT31340.13362394622979226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAAGATCGGAAGAGCACACGTCT31260.1332828512808968Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTCTGAACT30500.13004244926639005Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGAGATCGGAAGAGCACACGTC30380.12953080684304685No Hit
GGAGGTGGAGCTAGAGCGCGTGCGATGAGATCGGAAGAGCACACGTCTGA30230.1288912538138679Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGT29800.12705786846355485No Hit
CCAGGGGCGAGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC29370.1252244831132418Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG29360.12518184624462989Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC29290.12488338816434637No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC28500.12151507554400381No Hit
ACACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC28320.12074761190898904No Hit
CCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGAA28180.12015069574842202Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGGTGGAGCTAGAGCGCGTGCGATGGAGATCGGAAGAGCACACGTCTG28010.11942586898201918Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCT27740.11827467352949704Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGTGGACGGTGTGAGGCCGGTAACGAGATCGGAAGAGCACACGTCTGA26750.11405362353691585Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCGATTGGCCTGGGATAGCCGGCCGAGATCGGAAGAGCACACGTCTGA26740.11401098666830392Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGGGCCCGCGCCCTGGAAAGCGCCGCGAGATCGGAAGAGCACACGTC26520.11307297555884144No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT26340.11230551192382668Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA26040.11102640586546873Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGGGGCGAGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACT25940.11060003717934942Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGCGAGAACTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAAC25770.10987521041294661Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTG25540.10889456243487218Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCCCTCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT25420.10838292001152901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGA25120.10710381395317106Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGTGGACGGTGTGAGGCCGGTAACAGATCGGAAGAGCACACGTCTGAA25120.10710381395317106Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTGAA25060.10684799274149949Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA24510.10450296496784327Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCGGGGACGCGTGCATTTATCAAGATCGGAAGAGCACACGTCTGAACT24490.1044176912306194Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCT23560.1004524624497098Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCTTCGATTGGCCTGGGATAGCCGGCCGAGATCGGAAGAGCACACGTC23550.10040982558109789No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)