FastQCFastQC Report
Thu 28 Sept 2023
SRR1062585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG905002.2624999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTGAGATCGGAAGAGCACACGTCTG316670.791675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG242510.606275TruSeq Adapter, Index 11 (100% over 49bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA236220.59055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAACAGATCGGAAGAGCACACGTCTG225310.563275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC222040.5551No Hit
ACACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG212750.531875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC196040.4901No Hit
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA189690.474225Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG177490.44372500000000004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA163300.40825Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC150100.37525000000000003No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG142730.356825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGAGTCGGGTTCAGATCCCCGAACCCGGAGAGATCGGAAGAGCACACG140630.351575No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA139730.349325Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAAAGATCGGAAGAGCACACGTCTGA117800.29450000000000004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAACGAGATCGGAAGAGCACACGTCT115110.287775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT112930.282325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA112920.2823Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG112210.280525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT110970.277425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGT105730.26432500000000003No Hit
CCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGAA105380.26345Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT98500.24625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTGAA93550.233875Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCGGGGACGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC90240.22560000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTGA87960.2199Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTG82250.205625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCTGGTTGATCCTGCCAGTAACAGATCGGAAGAGCACACGTCTGAACT82130.205325Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCTAGATAACCTCGGGCCGATCGCGCAGATCGGAAGAGCACACGTCTG81890.204725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAACGGCAGATCGGAAGAGCACACGT79860.19965No Hit
AACTCTTAGCGGTGGATCACTCGGAGATCGGAAGAGCACACGTCTGAACT79670.199175Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT77750.194375No Hit
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT77540.19385Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTCTAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTCTGAAC75590.188975Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCCGTGGACGGTGTGAGGCCGGTAAGATCGGAAGAGCACACGTCTGAA75270.188175Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTGA71900.17975Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG69950.174875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGACCTCAGATCAGACGAGACAACCCGCAGATCGGAAGAGCACACGTCT69750.174375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC69410.173525Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGACCTCAGATCAGACGAGACAACCCGCTAGATCGGAAGAGCACACGTC69110.17277499999999998No Hit
ACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGA68100.17025Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTAGATCGGAAGAGCACACGTCTGA66930.167325Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT66460.16615Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGTCGGGTTCAGATCCCCGAACCCGGAGAGATCGGAAGAGCACACGT65970.16492500000000002No Hit
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT65020.16255Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA64890.162225Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG63650.159125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGA63340.15835000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCCCCTCGCCCCGACTTCGCCGCTTGAGATCGGAAGAGCACACGTCTG63120.1578Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT62370.155925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG60930.15232500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGACCTCAGATCAGACGAGACAACCCGAGATCGGAAGAGCACACGTCTG58840.1471Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGAAC58670.146675Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC55340.13835No Hit
GCTTACGGCCATACCACCCTGAGCACGCCCAGATCGGAAGAGCACACGTC55120.1378No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT54990.137475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGT54660.13665000000000002No Hit
CCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGAA54280.1357Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT53910.134775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGAGATCGGAAGAGCACACGTC53610.134025No Hit
GTTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTG53450.133625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGGGCGAGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC52950.132375Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAGGTGGAGCTAGAGCGCGTGCGATGAGATCGGAAGAGCACACGTCTGA52560.13140000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAAGATCGGAAGAGCACACGTCT51270.12817499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC50230.125575No Hit
GTCTAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTCTGAACT50100.12525Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG49660.12415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT49150.122875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGTGGACGGTGTGAGGCCGGTAACGAGATCGGAAGAGCACACGTCTGA48700.12175000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCCCCTCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT48620.12155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGGTGGAGCTAGAGCGCGTGCGATGGAGATCGGAAGAGCACACGTCTG48120.1203Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGGGGACGCGTGCATTTATCAAGATCGGAAGAGCACACGTCTGAACT47960.11989999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGTGGACGGTGTGAGGCCGGTAACAGATCGGAAGAGCACACGTCTGAA47690.119225Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC47330.118325No Hit
CAGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTG47320.1183Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGCGAGAACTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAAC47280.11820000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGGGGCCCGCGCCCTGGAAAGCGCCGCGAGATCGGAAGAGCACACGTC47130.117825No Hit
AGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGA46920.1173Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGGGGCGAGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACT45780.11445Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCT44890.11222499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGATTGGCCTGGGATAGCCGGCCGAGATCGGAAGAGCACACGTCTGA44690.11172499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA44080.11019999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA43300.10824999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTTCGGGGGCGAAGGTGGCTCGCGGCTAGATCGGAAGAGCACACGTCTG42670.10667499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGGGGACGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC42510.106275Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC42140.10535No Hit
TGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTGAA42030.105075Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCTTCGATTGGCCTGGGATAGCCGGCCGAGATCGGAAGAGCACACGTC41540.10385No Hit
GGGTCTAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTCTGAA41510.103775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT41410.10352499999999999No Hit
CACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCT41300.10325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG40290.100725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG40260.10065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)