FastQCFastQC Report
Thu 28 Sept 2023
SRR1062631.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062631.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG902792.2569749999999997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTGAGATCGGAAGAGCACACGTCTG325170.8129249999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG255420.63855TruSeq Adapter, Index 11 (100% over 49bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA233620.5840500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC229360.5734No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAACAGATCGGAAGAGCACACGTCTG226260.56565Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG208100.52025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC198390.495975No Hit
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA187130.467825Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG178090.445225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA165740.41434999999999994Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC149840.37460000000000004No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA146240.3656Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG145880.3647Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGAGTCGGGTTCAGATCCCCGAACCCGGAGAGATCGGAAGAGCACACG143950.359875No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAAAGATCGGAAGAGCACACGTCTGA119220.29805Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAACGAGATCGGAAGAGCACACGTCT117640.29410000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG112890.282225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT112860.28214999999999996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA111940.27985000000000004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT111550.278875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGAA108710.271775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGT107130.267825No Hit
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT98360.24589999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTGA92510.231275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTGAA92360.2309Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCGGGGACGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC90210.22552499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCTAGATAACCTCGGGCCGATCGCGCAGATCGGAAGAGCACACGTCTG83460.20864999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCTGGTTGATCCTGCCAGTAACAGATCGGAAGAGCACACGTCTGAACT83060.20764999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTG81440.2036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAACGGCAGATCGGAAGAGCACACGT81370.203425No Hit
AACTCTTAGCGGTGGATCACTCGGAGATCGGAAGAGCACACGTCTGAACT80860.20215Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT77530.193825No Hit
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT77390.193475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAAGATCGGAAGAGCACACGTCTGAA76040.1901Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTCTAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTCTGAAC75850.189625Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC71920.1798Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG70830.177075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTGA70280.17570000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGACCTCAGATCAGACGAGACAACCCGCTAGATCGGAAGAGCACACGTC69030.172575No Hit
ACGACCTCAGATCAGACGAGACAACCCGCAGATCGGAAGAGCACACGTCT67970.169925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTAGATCGGAAGAGCACACGTCTGA67350.168375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT66690.16672499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGA66630.166575Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT66340.16585Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA65520.1638Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCCCCTCGCCCCGACTTCGCCGCTTGAGATCGGAAGAGCACACGTCTG65440.1636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGTCGGGTTCAGATCCCCGAACCCGGAGAGATCGGAAGAGCACACGT65240.1631No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG64680.16169999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGA63800.1595Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT62570.156425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG61460.15365Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGAAC60620.15155Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGACCTCAGATCAGACGAGACAACCCGAGATCGGAAGAGCACACGTCTG59900.14975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC55480.1387No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGT55340.13835No Hit
CCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGAA55220.13805Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT55140.13785Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGAGATCGGAAGAGCACACGTC54840.1371No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT54690.13672499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTACGGCCATACCACCCTGAGCACGCCCAGATCGGAAGAGCACACGTC54360.1359No Hit
CCAGGGGCGAGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC53800.1345Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTG52730.131825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGGTGGAGCTAGAGCGCGTGCGATGAGATCGGAAGAGCACACGTCTGA52340.13085Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAAGATCGGAAGAGCACACGTCT51200.128Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT50330.125825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC50030.125075No Hit
CCCGTGGACGGTGTGAGGCCGGTAACGAGATCGGAAGAGCACACGTCTGA50000.125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCCCCTCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT49770.124425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTCTGAACT49180.12294999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC49180.12294999999999999No Hit
GGAGGTGGAGCTAGAGCGCGTGCGATGGAGATCGGAAGAGCACACGTCTG48810.122025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG48670.12167499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGGGGACGCGTGCATTTATCAAGATCGGAAGAGCACACGTCTGAACT47980.11995Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTG46740.11685000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCT46220.11555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGTGGACGGTGTGAGGCCGGTAACAGATCGGAAGAGCACACGTCTGAA46010.115025Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGGGGCGAGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACT45710.11427500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGGGGCCCGCGCCCTGGAAAGCGCCGCGAGATCGGAAGAGCACACGTC45640.11410000000000001No Hit
CTTCGATTGGCCTGGGATAGCCGGCCGAGATCGGAAGAGCACACGTCTGA45320.11329999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGA44800.11199999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGCGAGAACTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAAC44630.111575Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA44610.11152499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA43630.10907499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC43430.10857499999999999No Hit
GGTTCGGGGGCGAAGGTGGCTCGCGGCTAGATCGGAAGAGCACACGTCTG43140.10785Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCTTCGATTGGCCTGGGATAGCCGGCCGAGATCGGAAGAGCACACGTC42930.107325No Hit
TGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTGAA42830.107075Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCGGGGACGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC41860.10464999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT41600.104No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT40730.101825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCT40690.101725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCT40220.10055000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)