FastQCFastQC Report
Thu 28 Sept 2023
SRR1062635.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062635.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG919522.2988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTGAGATCGGAAGAGCACACGTCTG323510.8087749999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG249510.623775TruSeq Adapter, Index 11 (100% over 49bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA240330.6008249999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC239200.598No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAACAGATCGGAAGAGCACACGTCTG225790.564475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG212670.531675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG184220.46055Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC182460.45614999999999994No Hit
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA180040.4501Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA167700.41925Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG150250.375625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC148320.3708No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA138420.34605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGAGTCGGGTTCAGATCCCCGAACCCGGAGAGATCGGAAGAGCACACG129140.32285No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAACGAGATCGGAAGAGCACACGTCT119600.299Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAAAGATCGGAAGAGCACACGTCTGA118140.29535Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA116390.290975Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT114760.2869Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT112470.281175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG110670.276675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGAA109850.274625Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGGGGGGCGCACCACCGGCCCGTCTCGTAGATCGGAAGAGCACACGT95690.239225No Hit
ACTTCGAACGCACTTTGCGGCCCCGGAGATCGGAAGAGCACACGTCTGAA92960.23240000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCCTACGTCCTCCTAAGCGCAGACCGAGATCGGAAGAGCACACGTCT92240.2306Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCGGGGACGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC90850.227125Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCTGGTTGATCCTGCCAGTAACAGATCGGAAGAGCACACGTCTGAACT85050.212625Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCTAGATAACCTCGGGCCGATCGCGCAGATCGGAAGAGCACACGTCTG84840.2121Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTCTTAGCGGTGGATCACTCGGAGATCGGAAGAGCACACGTCTGAACT84630.211575Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTG82780.20695000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTGA82080.2052Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAACGGCAGATCGGAAGAGCACACGT81550.20387500000000003No Hit
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT79030.197575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT77390.193475No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAAGATCGGAAGAGCACACGTCTGAA76940.19235000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTCTAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTCTGAAC75260.18814999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC73890.184725Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTGA73690.184225Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG70940.17734999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTCGGTGCGGAGAGCCAGCCGTAGATCGGAAGAGCACACGTCTGA68840.1721Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT67820.16955Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT67430.168575Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCTCGGTCGGTGCGGAGAGCCAGCCGAGATCGGAAGAGCACACGTCTGA66000.165Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCGAACGCACTTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGA65520.1638Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGACCTCAGATCAGACGAGACAACCCGCAGATCGGAAGAGCACACGTCT64900.16225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGACCTCAGATCAGACGAGACAACCCGCTAGATCGGAAGAGCACACGTC64590.161475No Hit
CGCCCCCTCGCCCCGACTTCGCCGCTTGAGATCGGAAGAGCACACGTCTG64170.160425Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT61680.1542Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGTCGGGTTCAGATCCCCGAACCCGGAGAGATCGGAAGAGCACACGT61550.15387499999999998No Hit
CGGGGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGA61340.15335Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGAAC61280.1532Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG61270.15317499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG60320.1508Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGACCTCAGATCAGACGAGACAACCCGAGATCGGAAGAGCACACGTCTG57250.143125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGT56620.14155No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC55820.13955No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT55210.138025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT54870.137175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGGTGGAGCTAGAGCGCGTGCGATGAGATCGGAAGAGCACACGTCTGA54410.13602499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTACGGCCATACCACCCTGAGCACGCCCAGATCGGAAGAGCACACGTC54310.135775No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT53490.13372499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGAGATCGGAAGAGCACACGTC53240.1331No Hit
CCAGGGGCGAGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC53090.132725Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTTCCTCACTTACCCGGTTGGGCAGGGAGATCGGAAGAGCACACGTCTG51960.12990000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC50910.127275No Hit
CCCGTGGACGGTGTGAGGCCGGTAACGAGATCGGAAGAGCACACGTCTGA50860.12715Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAAGATCGGAAGAGCACACGTCT50830.127075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC50820.12705No Hit
CCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGAA50540.12635000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG50090.125225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCCCTCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT49980.12494999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGGTGGAGCTAGAGCGCGTGCGATGGAGATCGGAAGAGCACACGTCTG49970.124925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGTGGACGGTGTGAGGCCGGTAACAGATCGGAAGAGCACACGTCTGAA49710.124275Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAAGTCCTTCTGATGGAGGCTAGATCGGAAGAGCACACGTCTGAACT49400.1235Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCGGGGACGCGTGCATTTATCAAGATCGGAAGAGCACACGTCTGAACT48240.1206Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGGGGCCCGCGCCCTGGAAAGCGCCGCGAGATCGGAAGAGCACACGTC46170.11542499999999999No Hit
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA45680.1142Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCGATTGGCCTGGGATAGCCGGCCGAGATCGGAAGAGCACACGTCTGA44740.11184999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTG44680.1117Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGA44570.111425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCT43800.1095Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTGAA43340.10835Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA42880.1072Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGCGAGAACTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAAC42700.10675000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGGGGCGAGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACT42580.10644999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCGGGGACGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTC42310.10577500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTTCGAACGCACTTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCT41680.1042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTTCGGGGGCGAAGGTGGCTCGCGGCTAGATCGGAAGAGCACACGTCTG41420.10355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACTC40040.1001Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)