FastQCFastQC Report
Thu 28 Sept 2023
SRR1062648.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062648.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG852062.13015No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG804032.010075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG789191.9729750000000001No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT785711.964275No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT709491.773725No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT696351.740875No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC596481.4912No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA579401.4485Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG564041.4101000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG443721.1093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC422971.057425No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC366360.9159No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT361480.9037Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGT359290.898225No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG352690.8817250000000001TruSeq Adapter, Index 12 (100% over 49bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC341830.854575No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT337420.84355Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT324000.8099999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG273560.6839Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC255790.639475No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT253080.6327Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG251560.6289Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT249830.624575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAA244620.61155Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT243900.60975No Hit
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC240570.601425Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA238420.59605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT219650.549125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC213130.532825No Hit
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC194500.48624999999999996No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC189840.4746No Hit
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA186050.465125Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC182740.45685No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT180030.45007500000000006Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT173650.434125No Hit
AACGATGCCGACCCGCGATCCGGCGGCGTTAGATCGGAAGAGCACACGTC166040.4151No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT162010.405025No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC151460.37865No Hit
CCCCTGTCCGCGGCTGGAGCGTCGCAGGCCAGATCGGAAGAGCACACGTC146040.36510000000000004No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGAGATCGGAAGAGCACACGTC137350.34337500000000004No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA132550.331375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACGT130750.32687499999999997No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCTG130190.32547499999999996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT120440.3011Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGTC120220.30055No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT118380.29595No Hit
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA117080.2927Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTC111050.277625No Hit
TAAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTC108800.272No Hit
CCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCT108630.271575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT107110.267775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG106880.2672Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC105170.262925No Hit
ACCCGGGGACGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC103300.25825000000000004Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTG101580.25395Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTCGGCGCGGGCCGGGGGGCCAAAAGATCGGAAGAGCACACGTCTGA93640.23410000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACG93360.23340000000000002No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCAGATCGGAAGAGCACACG87750.219375No Hit
GGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACGT87210.218025No Hit
ACGATGCCGACCCGCGATCCGGCGGCGTTAGATCGGAAGAGCACACGTCT86740.21685Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC86640.2166No Hit
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG86090.215225No Hit
ATCCCGGGGCCACGCCTGTCTGAGGGTCGAGATCGGAAGAGCACACGTCT86040.2151Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG82380.20595000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTG79810.199525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCT76750.19187500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACG75920.1898No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG73610.18402500000000002No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG71800.1795No Hit
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG67530.168825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCT67250.168125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAAGATCGGAAGAGCACACG67180.16795No Hit
TCTCCGGGGCCGCGGGTGAGTGTCCTCCGAGATCGGAAGAGCACACGTCT65700.16425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT65020.16255Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAACCATCTAGTAGCTGGTTCCCTCCGAAGAGATCGGAAGAGCACACGTC64940.16235No Hit
TTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACG63150.15787500000000002No Hit
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC62580.15644999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC62010.155025No Hit
AGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTGAAC61650.154125Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGA61230.153075Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG59620.14905000000000002No Hit
TCGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC59090.147725No Hit
CGAACCATCTAGTAGCTGGTTCCCTCCGAAGAGATCGGAAGAGCACACGT58660.14665No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACGTCTGAAC58490.146225Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAAGATCGGAAGAGCACACGTCTGAA57600.14400000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGA56400.14100000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCCTCCCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT55640.1391Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACCCGCGATCCGGCGGCGTCAGATCGGAAGAGCACACGTC55360.1384No Hit
ACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTGA54250.135625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTCT53090.132725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGAAGATCGGAAGAGCACACGT52980.13244999999999998No Hit
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA52780.13194999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT52420.13105Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCCGGCGCGGAGAGCCATTCGTGACAGGAGATCGGAAGAGCACACGTC52130.130325No Hit
GGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA51670.12917499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAACT51590.128975Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACGTCT51450.12862500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG51210.128025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT51160.12789999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGAGATCGGAAGAGCACACGTCT50970.127425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTCTACAGTCCGACGATCAAACAGATCGGAAGAGCACACGTCTGAACT50860.12715Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT50380.12595Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCTGAA50290.125725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG48480.1212Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTCT48200.1205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCTGAA46850.11712499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGAGATCGGAAGAGCACACGTCTG46550.116375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACGT46050.11512499999999999No Hit
CGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTGA46040.1151Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT45910.11477499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGAGATCGGAAGAGCACACGTC44940.11234999999999999No Hit
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT44930.112325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCTG44280.11069999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACAC43490.10872499999999999No Hit
AGACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTC43480.1087No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG43040.10759999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT41720.1043Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGCTCGCGAGCCCCTGCGGCGCAATGAAGAGATCGGAAGAGCACACG40460.10115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)