FastQCFastQC Report
Thu 28 Sept 2023
SRR1062727.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062727.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG875692.189225No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG835482.0887No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT822202.0555No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG820542.0513500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT743761.8594No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT720401.8010000000000002No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC619801.5495No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA609361.5233999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG564911.412275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC452401.131No Hit
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG449101.12275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC392450.981125No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT375660.93915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGT369950.924875No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG359640.8991000000000001TruSeq Adapter, Index 12 (100% over 49bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC354850.887125No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT346580.86645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT324290.810725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG273270.683175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT266770.666925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC258350.645875No Hit
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG255040.6376Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT249700.62425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAA247970.619925Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC247640.6191Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT243530.608825No Hit
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA241960.6049Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT232000.58Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC225450.5636249999999999No Hit
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA211250.5281250000000001Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC202790.506975No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC193560.48390000000000005No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC185640.4641No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT185480.46369999999999995No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT181440.4536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACCCGCGATCCGGCGGCGTTAGATCGGAAGAGCACACGTC169600.424No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT169530.423825No Hit
CCCCTGTCCGCGGCTGGAGCGTCGCAGGCCAGATCGGAAGAGCACACGTC157760.3944No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC147930.369825No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGAGATCGGAAGAGCACACGTC143780.35945No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCTG137850.344625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACGT132390.330975No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA131030.327575Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGTC123140.30785No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT123110.307775No Hit
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT117530.293825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTC117310.293275No Hit
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA115420.28855000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCT113840.2846Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG108550.271375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTG107370.268425Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTC106690.266725No Hit
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT105250.263125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC104340.26085No Hit
ACCCGGGGACGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC103600.259Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACG96550.241375No Hit
TCTCTCGGCGCGGGCCGGGGGGCCAAAAGATCGGAAGAGCACACGTCTGA92440.2311Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCAGATCGGAAGAGCACACG92120.2303No Hit
GGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACGT88050.22012500000000002No Hit
ATCCCGGGGCCACGCCTGTCTGAGGGTCGAGATCGGAAGAGCACACGTCT88000.22Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATGCCGACCCGCGATCCGGCGGCGTTAGATCGGAAGAGCACACGTCT87490.218725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC87150.217875No Hit
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG86590.216475No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG80350.20087500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTG80230.200575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACG79670.199175No Hit
CGAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCT78540.19635Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG76760.1919No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG76420.19105No Hit
GCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCT72020.18005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAAGATCGGAAGAGCACACG70190.175475No Hit
TCTCCGGGGCCGCGGGTGAGTGTCCTCCGAGATCGGAAGAGCACACGTCT68870.172175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG66760.1669Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT65650.164125Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAACCATCTAGTAGCTGGTTCCCTCCGAAGAGATCGGAAGAGCACACGTC63740.15935000000000002No Hit
AGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGA63070.157675Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC62880.1572No Hit
TTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACG62670.156675No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG61810.154525No Hit
AGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTGAAC61430.153575Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC61010.152525Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACGTCTGAAC60650.151625Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCCTCCCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT59700.14925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC58110.14527500000000002No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAAGATCGGAAGAGCACACGTCTGAA57880.1447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAACCATCTAGTAGCTGGTTCCCTCCGAAGAGATCGGAAGAGCACACGT57710.14427500000000001No Hit
CTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGA56230.140575Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTGA55590.138975Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATGCCGACCCGCGATCCGGCGGCGTCAGATCGGAAGAGCACACGTC55300.13825No Hit
AGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTCT54790.136975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCCGGCGCGGAGAGCCATTCGTGACAGGAGATCGGAAGAGCACACGTC54440.1361No Hit
GGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA53910.134775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGAAGATCGGAAGAGCACACGT53480.1337No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGAGATCGGAAGAGCACACGTCT53260.13315Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT52820.13205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA52610.131525Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT52000.13Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCTGAA51240.1281Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTCT50910.127275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAACT50890.127225Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACGTCT50270.125675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT50220.12555000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG49720.1243Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTCTACAGTCCGACGATCAAACAGATCGGAAGAGCACACGTCTGAACT49220.12305Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGAGATCGGAAGAGCACACGTCTG48840.12210000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCTGAA48800.122Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG48700.12175000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT47700.11925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCTG46470.116175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTGA45400.11349999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACGT45340.11334999999999999No Hit
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT45150.112875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACAC44600.11150000000000002No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGAGATCGGAAGAGCACACGTC44000.11No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG43760.1094Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTC43640.1091No Hit
GGTGGCTCGCGAGCCCCTGCGGCGCAATGAAGAGATCGGAAGAGCACACG43530.108825No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCAGATCGGAAGAGCACACGT42440.10610000000000001No Hit
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT40840.1021Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCT40370.100925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)