FastQCFastQC Report
Thu 28 Sept 2023
SRR1062729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG835262.08815No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG796021.99005No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG765251.913125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT765141.91285No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT696201.7405No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT681651.7041250000000001No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC576011.4400249999999999No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA549451.373625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG545211.363025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG444971.112425Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC417171.0429249999999999No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC370750.9268749999999999No Hit
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGT365020.91255No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT354160.8854000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG353670.884175TruSeq Adapter, Index 12 (100% over 49bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC340310.850775No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT339590.848975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT322240.8056000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG282960.7074Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC261940.6548499999999999No Hit
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT257920.6447999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG251700.6292500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT240680.6017Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC240120.6003Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT237630.594075No Hit
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA237420.59355Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAA232470.581175Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT209450.523625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC207090.517725No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC195260.48815000000000003No Hit
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC183540.45885No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC182670.45667499999999994No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT173430.43357500000000004No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT171390.42847500000000005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA166610.416525Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACCCGCGATCCGGCGGCGTTAGATCGGAAGAGCACACGTC165860.41465No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT158950.397375No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC152070.380175No Hit
CCCCTGTCCGCGGCTGGAGCGTCGCAGGCCAGATCGGAAGAGCACACGTC136770.341925No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGAGATCGGAAGAGCACACGTC136510.341275No Hit
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACGT132490.331225No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA129620.32405Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCTG128600.3215Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGTC124450.311125No Hit
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT120910.30227499999999996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA119150.297875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT116650.291625No Hit
GCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTC114100.28525No Hit
TAAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTC110380.27595No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC108340.27085No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG107920.2698Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT105970.264925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCT102900.25725000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCGGGGACGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC98980.24745Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTG95060.23765000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACG94080.2352No Hit
TCTCTCGGCGCGGGCCGGGGGGCCAAAAGATCGGAAGAGCACACGTCTGA93810.234525Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC89020.22255No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCAGATCGGAAGAGCACACG86780.21695No Hit
ATCCCGGGGCCACGCCTGTCTGAGGGTCGAGATCGGAAGAGCACACGTCT85880.2147Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACGT84260.21064999999999998No Hit
ACGATGCCGACCCGCGATCCGGCGGCGTTAGATCGGAAGAGCACACGTCT84000.21Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG81040.2026No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG80500.20124999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTG79300.19824999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCT75370.188425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACG71860.17965No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG71710.17927500000000002No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG69610.174025No Hit
GCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCT69390.173475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG68900.17225000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAAGATCGGAAGAGCACACG67670.169175No Hit
TCTCCGGGGCCGCGGGTGAGTGTCCTCCGAGATCGGAAGAGCACACGTCT66310.165775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT65950.164875Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAACCATCTAGTAGCTGGTTCCCTCCGAAGAGATCGGAAGAGCACACGTC64890.162225No Hit
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC63710.159275Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC63590.158975No Hit
AGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTGAAC63240.1581Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGA61450.153625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACG61390.153475No Hit
CTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGA59010.147525Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAACCATCTAGTAGCTGGTTCCCTCCGAAGAGATCGGAAGAGCACACGT58530.14632499999999998No Hit
TCGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC58490.146225No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG57910.14477500000000001No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT56470.141175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACGTCTGAAC56300.14075Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAAGATCGGAAGAGCACACGTCTGAA55270.138175Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG55070.137675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGCCGACCCGCGATCCGGCGGCGTCAGATCGGAAGAGCACACGTC55000.13749999999999998No Hit
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT52520.1313Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCTCCCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT52510.131275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAACT52500.13125Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACGTCT52390.13097499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA52320.1308Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGAAGATCGGAAGAGCACACGT52250.130625No Hit
AGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTCT51080.12769999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTGA50900.12725Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT50770.126925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCTGAA50720.1268Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCTACAGTCCGACGATCAAACAGATCGGAAGAGCACACGTCTGAACT49720.1243Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGAGATCGGAAGAGCACACGTCT48590.12147499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA48510.12127500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCCGGCGCGGAGAGCCATTCGTGACAGGAGATCGGAAGAGCACACGTC48130.12032499999999999No Hit
CGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCTGAA47330.118325Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG47240.1181Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTCT46980.11745Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT45940.11485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGAGATCGGAAGAGCACACGTCTG45720.11429999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTGA45000.11249999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT44210.11052499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACGT44130.110325No Hit
TTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACAC44100.11025No Hit
CCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCTG43840.1096Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGAGATCGGAAGAGCACACGTC43360.1084No Hit
AGACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTC42780.10694999999999999No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG42050.10512500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT41670.10417499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCATCTAGTAGCTGGTTCCCTCCGAAGAGATCGGAAGAGCACACGTCT41320.1033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)