FastQCFastQC Report
Thu 28 Sept 2023
SRR1062732.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062732.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG878872.197175No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT861352.153375No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG860432.151075No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG799581.99895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT747431.868575No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT734781.83695No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC615161.5379No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA596881.4922Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG549211.373025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC478011.195025No Hit
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG447881.1197000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC396430.9910749999999999No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT377140.94285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGT368580.9214500000000001No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG358190.8954749999999999TruSeq Adapter, Index 12 (100% over 49bp)
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC350770.876925No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT345030.862575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT323410.808525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG271750.6793750000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT267140.66785Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC251460.62865No Hit
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG250780.62695Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC245570.6139249999999999Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT245390.613475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA242630.606575Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAA241150.602875Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT237340.59335Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT236770.591925No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC224130.560325No Hit
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA213420.53355Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC193590.48397500000000004No Hit
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC192220.48055No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT185000.46249999999999997No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC182400.45599999999999996No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT180500.45125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACCCGCGATCCGGCGGCGTTAGATCGGAAGAGCACACGTC172050.43012500000000004No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT170350.42587500000000006No Hit
CCCCTGTCCGCGGCTGGAGCGTCGCAGGCCAGATCGGAAGAGCACACGTC160540.40135No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC149850.374625No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGAGATCGGAAGAGCACACGTC146430.366075No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCTG146000.365Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACGT136370.340925No Hit
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA128230.320575Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGTC123300.30825No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT121990.304975No Hit
GCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTC115950.289875No Hit
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA115750.289375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCT115600.28900000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT115240.28809999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTG109930.274825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTC108980.27244999999999997No Hit
ACCCGGGGACGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC106750.26687500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT105200.263Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG104970.26242499999999996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC103520.25880000000000003No Hit
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACG96230.24057499999999998No Hit
TCTCTCGGCGCGGGCCGGGGGGCCAAAAGATCGGAAGAGCACACGTCTGA94230.23557500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCAGATCGGAAGAGCACACG91750.22937500000000002No Hit
ATCCCGGGGCCACGCCTGTCTGAGGGTCGAGATCGGAAGAGCACACGTCT88230.220575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACGT86730.216825No Hit
ACGATGCCGACCCGCGATCCGGCGGCGTTAGATCGGAAGAGCACACGTCT86080.21519999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC85780.21445No Hit
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG85540.21385No Hit
CGCCGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACG82520.2063No Hit
AACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTG80930.20232499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG80530.20132499999999998No Hit
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG78250.19562500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG77440.1936No Hit
CGAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCT75440.18860000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCT71570.178925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAAGATCGGAAGAGCACACG68460.17115No Hit
TCTCCGGGGCCGCGGGTGAGTGTCCTCCGAGATCGGAAGAGCACACGTCT67060.16765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT66050.165125Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG64480.16119999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACCATCTAGTAGCTGGTTCCCTCCGAAGAGATCGGAAGAGCACACGTC62690.156725No Hit
TTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACG62600.1565No Hit
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC62280.1557No Hit
AGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGA60990.152475Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCCTCCCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT60810.152025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACGTCTGAAC60630.151575Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC60180.15045Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG59480.1487No Hit
TCGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC59440.14859999999999998No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAAGATCGGAAGAGCACACGTCTGAA58340.14585Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTGAAC58200.1455Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAACCATCTAGTAGCTGGTTCCCTCCGAAGAGATCGGAAGAGCACACGT57860.14465No Hit
AACGATGCCGACCCGCGATCCGGCGGCGTCAGATCGGAAGAGCACACGTC55830.139575No Hit
CTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGA54600.13649999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGAGATCGGAAGAGCACACGTCT54090.13522499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTCT53290.133225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTGA53280.1332Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGAAGATCGGAAGAGCACACGT53210.133025No Hit
CCCCCGGCGCGGAGAGCCATTCGTGACAGGAGATCGGAAGAGCACACGTC52990.132475No Hit
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT52630.131575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACGTCT52150.130375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA51570.128925Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA51380.12845Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT50840.1271Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCTGAA50670.126675Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT49760.12440000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTCT49590.123975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAACT49250.123125Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT49090.122725Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG49050.12262499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGAGATCGGAAGAGCACACGTCTG49010.122525Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTCTACAGTCCGACGATCAAACAGATCGGAAGAGCACACGTCTGAACT48220.12054999999999999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCTGAA47940.11984999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG46550.116375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT45040.1126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCTG44820.11205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACGT44260.11065000000000001No Hit
CGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTGA44070.11017500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG43220.10805000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGAGATCGGAAGAGCACACGTC42860.10715No Hit
AGACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTC42850.107125No Hit
TTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACAC42390.10597499999999999No Hit
GGTGGCTCGCGAGCCCCTGCGGCGCAATGAAGAGATCGGAAGAGCACACG42300.10575000000000001No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCAGATCGGAAGAGCACACGT41620.10405No Hit
GTGGCTCGCGAGCCCCTGCGGCGCAATGAAGAGATCGGAAGAGCACACGT40310.100775No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)