FastQCFastQC Report
Thu 28 Sept 2023
SRR1062813.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1062813.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT985152.462875No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG977552.443875No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG957432.393575No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT839202.098No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT822122.0553No Hit
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG809362.0233999999999996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC646821.61705No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA622551.5563749999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC568401.421No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG566531.416325Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG468741.17185Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC437141.09285No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG399460.99865TruSeq Adapter, Index 12 (100% over 49bp)
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT395700.9892500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGT369830.924575No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC363480.9087No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT358360.8959Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT333420.8335499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT290530.726325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT286360.7159000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG267670.669175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG260040.6501Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA257010.642525Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC254250.635625No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC251560.6289No Hit
GGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAA249500.62375Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT245790.614475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT245300.61325No Hit
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC244270.610675Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA216800.542Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT208330.520825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCTGTCCGCGGCTGGAGCGTCGCAGGCCAGATCGGAAGAGCACACGTC203160.5079No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT198020.49505No Hit
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC197810.494525No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC196730.491825No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC183620.45905No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT174940.43734999999999996No Hit
AACGATGCCGACCCGCGATCCGGCGGCGTTAGATCGGAAGAGCACACGTC173740.43435No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCTG165170.412925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGAGATCGGAAGAGCACACGTC154140.38535No Hit
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC145560.3639No Hit
CCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCT142770.356925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACGT139820.34954999999999997No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT136290.340725No Hit
CCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTG135680.3392Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA131960.32989999999999997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGTC130640.3266No Hit
GCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTC125950.314875No Hit
ACCCGGGGACGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAAC119820.29955Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT114310.285775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA111630.27907499999999996Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG104150.260375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT103300.25825000000000004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTC98250.245625No Hit
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACG98060.24515No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCAGATCGGAAGAGCACACG97510.243775No Hit
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC97380.24345No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG93370.233425No Hit
TCTCTCGGCGCGGGCCGGGGGGCCAAAAGATCGGAAGAGCACACGTCTGA91150.227875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATGCCGACCCGCGATCCGGCGGCGTTAGATCGGAAGAGCACACGTCT89320.22330000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG88900.22225No Hit
GGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACGT88230.220575No Hit
CGCCGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACG87990.21997499999999998No Hit
ATCCCGGGGCCACGCCTGTCTGAGGGTCGAGATCGGAAGAGCACACGTCT87100.21775000000000003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG84720.21180000000000002No Hit
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC83550.20887499999999998No Hit
AACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTG82990.20747500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCT80990.20247500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCT79570.19892500000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAATAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG74500.18625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCTCCCGCCCCGACTTCGCCGCTTGCAGATCGGAAGAGCACACGTCT71920.1798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAAGATCGGAAGAGCACACG70310.175775No Hit
GACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAACT68810.172025Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCGGGGCCGCGGGTGAGTGTCCTCCGAGATCGGAAGAGCACACGTCT68590.171475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACGTCTGAAC68030.170075Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAAGATCGGAAGAGCACACGTCTGAA65860.16465Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG63450.158625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCCGGCGCGGAGAGCCATTCGTGACAGGAGATCGGAAGAGCACACGTC62860.15715No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG62760.1569No Hit
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC60680.1517No Hit
GAACCATCTAGTAGCTGGTTCCCTCCGAAGAGATCGGAAGAGCACACGTC60650.151625No Hit
TTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACG60620.15155No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGAGATCGGAAGAGCACACGTCT60550.15137499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGA60350.150875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCTGAA59280.1482Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGTCT58500.14625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACCCGCGATCCGGCGGCGTCAGATCGGAAGAGCACACGTC58320.1458No Hit
AGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTCT57070.142675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTGAAC56240.1406Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC56050.140125Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAACCATCTAGTAGCTGGTTCCCTCCGAAGAGATCGGAAGAGCACACGT55940.13985No Hit
ACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTGA55930.139825Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACGTCT53800.1345Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT53800.1345Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA53510.133775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGAAGATCGGAAGAGCACACGT52100.13025No Hit
CTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGA51770.129425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA51200.128Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCTG50590.126475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGAGATCGGAAGAGCACACGTCTG50380.12595Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCCATTGAATACCCCAGCTAGGAATAAGATCGGAAGAGCACACGTCT49730.124325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCT49340.12335Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT48930.12232499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTCTACAGTCCGACGATCAAACAGATCGGAAGAGCACACGTCTGAACT48110.120275Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTGA47130.117825Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGCTCGCGAGCCCCTGCGGCGCAATGAAGAGATCGGAAGAGCACACGT46900.11725No Hit
TCGTTTCCTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC46830.11707499999999998No Hit
ATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAACT46380.11595Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACGT46180.11545No Hit
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG45680.1142Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGCGGGGGGCGCACCACCGGCCCGCCTCGCAGATCGGAAGAGCACAC45550.113875No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCAGATCGGAAGAGCACACGT45000.11249999999999999No Hit
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT44590.111475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCTGAA44150.11037499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG43860.10965Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAACTGCGAATGGCTCATTAAATCAGAGATCGGAAGAGCACACGTCTG43790.109475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGCTCGCGAGCCCCTGCGGCGCAATGAAGAGATCGGAAGAGCACACG43720.1093No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGAGATCGGAAGAGCACACGTC43180.10794999999999999No Hit
TTGAACCCCGCCCGTGATGGGGACCGGGGATTGAGATCGGAAGAGCACAC41850.10462500000000001No Hit
AGACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTC40990.10247500000000001No Hit
GCCGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGT40720.1018No Hit
CGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCTG40670.101675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCT40600.1015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTGGTGGAGGCCCGCCGCGGTCCTGACGAGATCGGAAGAGCACACGTC40390.100975No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCCAGATCGGAAGAGCACAC40320.1008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)