FastQCFastQC Report
Tue 18 Jul 2023
SRR10669501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10669501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32613322
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length18-136
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCG7458552.286964204382491No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC7017762.1518077796551975No Hit
ACGTACGCAGTTTTATCCGGTAAAGCG5467241.676382430468138No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC5043661.5465029904037375No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT4397571.348396829982545No Hit
ACGTACGCAGTTTTATCCGGTAAAGC3306921.013978275503489No Hit
TCGACTGCATAATTTGTGGTAGTGGGG3075650.9430655362247367No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG2816520.8636102755800222No Hit
ATTGATCATCGACACTTCGAACGCA2699350.8276832393829736No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2629260.8061920217756412No Hit
ATGTTAGGGATAACAGGGTAATGCGA2601800.7977721496755222No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC2583630.7922008067746058No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2476560.7593706645400919No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAG2450060.751245150677996No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAG2345600.7192152949031073No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC2270630.696227756252491No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC1855190.5688442287479945No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG1748540.5361428682426157No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATT1546110.4740731410311406No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGC1526780.4681461152592796No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATT1393700.4273407045133274No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG1388530.4257554627523072No Hit
TTAATCCACCTATAACTTCTCTGTTAACCCA1325830.40653019033142346No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC1311660.40218534008893664No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCC1297380.3978067612983431No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG1283290.39348644090902485No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCC1227090.3762542190580892No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGC1217670.37336582884748754No Hit
ACGTACGCAGTTTTATCCGGTAAAG1142520.35032309802724176No Hit
ACGTACGCAGTTTTATCCGGTAAA1133570.3475788207040056No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGC1087320.3333974993409135No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCG1071380.3285099260970716No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG1049810.3218960644364901No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC991100.3038942184423899No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC913080.27997147913972087No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGC890210.2729590073651497No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG877470.2690526282480515No Hit
TTAATCCACCTATAACTTCTCTGTTAACCCAA830240.2545708161836442No Hit
TATTGATCATCGACACTTCGAACGCA819340.25122862368942356No Hit
TCTTTCGCCGAATCCCGGGGCCGAGGAAG815640.2500941179803763No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC803300.24631038812912096No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC769600.23597718748185176No Hit
TTTGATCATCGACACTTCGAACGCA752810.23082898454809356No Hit
ACGTACGCAGTTTTATCCGGTAA735890.22564092060293642No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG735070.22538948960795835No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAA694150.21284246971222373No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC692560.21235493888049797No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC681980.20911086579895174No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG678500.20804381718611797No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG672670.2062562041364569No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG669070.20515236074387025No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAA666750.20444099500198107No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGG666410.20433674312601455No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC658430.20188989027244758No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC646780.1983177304047714No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCA639600.19611617608289028No Hit
TCGACTGCATAATTTGTGGTAGTGGG634480.19454626548010043No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC619680.19000824264391097No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAA615470.18871735912091384No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT613610.18814704003474408No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT612040.18766564166631047No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGC609700.18694814346112917No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAG591750.1814442576564264No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG582370.17856813237240904No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGG578520.17738763318867057No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCG577730.17714540088863073No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG577650.17712087103546212No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCA570220.1748426609224292No Hit
TTCGACTGCATAATTTGTGGTAGTGGGG562600.17250619240812082No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAA561990.1723191522777103No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTG549600.16852009126822468No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA530050.1625256084001501No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCA522540.16022286843394856No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAG512650.1571903653359814No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAAAGAG511590.15686534478149758No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAG488260.14971182635120703No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC481100.14751640449261808No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGC469770.14404236403761628No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT468900.14377560188440786No Hit
GGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG458120.1404702041699401No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG452940.1388818961772738No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTG449660.13787617219736154No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG448030.13737637643905148No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC446770.1369900312516462No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC444750.13637065245913924No Hit
TTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG440360.13502457676651278No Hit
TCTTTCGCCGAATCCCGGGGCCGAGGAAGC437720.13421509161194925No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC435740.13360797774602662No Hit
AATTGATCATCGACACTTCGAACGCA432390.13258079014459184No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGG422930.12968013500740588No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATT402270.12334530042661708No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCG396190.1214810315858041No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG394460.1209505735110333No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGA390150.11962902767157543No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGG385680.11825842212578037No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC378900.11617951706974224No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGG365400.1120401043475424No Hit
TCTTTCGCCGAATCCCGGGGCCGAGGAAGCC364120.11164762669684493No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAG362450.11113556601195058No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGG361570.11086573762709606No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGG348540.1068704377922617No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC344610.10566540875535464No Hit
TTCAAAAAGTAAGTTTAGATTATAGCCAA340240.10432546552602033No Hit
TCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG332800.1020441891813413No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG330350.10129296242805316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)