FastQCFastQC Report
Tue 18 Jul 2023
SRR10669503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10669503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39353376
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length18-135
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTACGCAGTTTTATCCGGTAAAGCG5667721.4402118893179585No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC4764811.2107754109837996No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCC4716391.1984715110591782No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG4552751.1568893098269384No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCC4482471.1390306132820727No Hit
TCGACTGCATAATTTGTGGTAGTGGGG3107890.789739106500037No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC3042710.7731763597613582No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG2802640.7121726989826743No Hit
TTAATCCACCTATAACTTCTCTGTTAACCCA2796020.7104905053126827No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG2731900.6941971128474467No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC2652460.6740107888075473No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC2572000.6535652748064105No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC2444290.6211131670126598No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG2433380.6183408508586404No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2359560.5995826126835979No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT2337360.593941419409608No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG2099300.5334485153192449No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCA1928260.48998591632900823No Hit
TTAATCCACCTATAACTTCTCTGTTAACCCAA1842900.4682952740827115No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGC1798440.4569976410664233No Hit
ACGTACGCAGTTTTATCCGGTAAAGC1760100.44725514781755954No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGG1668660.4240195301160439No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1611290.40944136533546704No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAAAGAG1581430.40185370627414535No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG1478320.37565265048670793No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAA1394630.3543863682749861No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCA1392640.3538806937427681No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAG1344450.3416352386133276No Hit
ATGTTAGGGATAACAGGGTAATGCGA1293690.328736726424691No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGC1289440.32765676825286855No Hit
TTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG1256300.3192356355907051No Hit
TCTTTCGCCGAATCCCGGGGCCGAGGAAGCC1202860.3056561144843075No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGG1143120.2904757142055614No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1122490.28523347018563283No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG1121380.28495141052193335No Hit
ACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG1080310.27451520296505183No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1070600.27204781617719404No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTG997380.2534420426852324No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC963830.24491672582296367No Hit
TTCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGG960130.24397652694396538No Hit
TTCAAAAAGTAAGTTTAGATTATAGCCAAAAGAG940860.23907986953902No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAG933520.2372147182493314No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC914580.23240191642008046No Hit
TTCGACTGCATAATTTGTGGTAGTGGGG877180.22289828450804322No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATT852540.2166370681895246No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG815830.20730877066303027No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG773130.19645836738377923No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAG754730.19178278376930102No Hit
ACCGGCTCCGGGACGGCCGGGAAGGCCC753820.19155154566662844No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCA752740.191277109237083No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAG747770.1900141934455636No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG744310.1891349804397976No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAA715490.18181159349581597No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAG705120.17917649555656928No Hit
ACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGG681620.17320496213590417No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGC665690.16915702480010864No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG656080.1667150487927643No Hit
CCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG651850.1656401727770446No Hit
ACCGGCTCCGGGACGGCCGGGAAGGCC650570.1653149147864722No Hit
ATTGATCATCGACACTTCGAACGCA634300.16118058079693087No Hit
ACCGGCTCCGGGACGGCCGGGAAGGCCCG620040.15755700349571025No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGA596030.15145587509442646No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC587980.14941030726309223No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAGG580850.14759851861248194No Hit
CCTTTCGCCGAATCCCGGGGCCGAGGAAGCC578210.14692767400692638No Hit
TCTTTCGCCGAATCCCGGGGCCGAGGAAGC562170.14285178481256602No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG557860.14175658017243553No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAG541450.13758667109017533No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC534650.1358587380152595No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAG533850.13565545177115174No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCC511200.1298999099848511No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC508000.12908676500842012No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC500890.1272800585139125No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAG499120.12683028769882412No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGG498490.12667019978158928No Hit
GGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG496880.12626108621532242No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG496420.12614419662496046No Hit
TCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG492910.12525227822893772No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG479980.12196666430854623No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAA472610.1200938897847036No Hit
CCGAATCCCGGGGCCGAGGAAGCCA472180.1199846234284957No Hit
TTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGG468650.11908762287637024No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC466050.11842694258302007No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC461630.11730378608432476No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC457010.11612980802460252No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCG454930.11560126378992237No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG451900.11483131714036428No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATT451240.11466360598897539No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC450280.11441966249604608No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG447150.11362430506597451No Hit
TCGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGGAAG445570.11322281473386171No Hit
TTCAAAAAGTAAGTTTAGATTATAGCCAA438590.11144914225402161No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG432280.10984572200362175No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCA420550.1068650374493919No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG414570.10534547277468646No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAG413570.10509136496955178No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGA413150.10498463969139521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)