FastQCFastQC Report
Tue 18 Jul 2023
SRR10669504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10669504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41195764
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length18-135
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATT5760311.3982772597687472No Hit
TCGACTGCATAATTTGTGGTAGTGGGG4888351.1866147208727578No Hit
ACGTACGCAGTTTTATCCGGTAAAGCG4711021.143569032971448No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTG4576411.1108933433058796No Hit
ATGTTAGGGATAACAGGGTAATGCGA4324361.0497098682281996No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGG3391230.8231987152853871No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAAAGAG3144680.7633503289318775No Hit
TTAATCCACCTATAACTTCTCTGTTAACCCA3076030.7466859942201824No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2779900.6748023898767844No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG2549640.6189082935808643No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2378680.5774088811655489No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG2259850.5485636824213286No Hit
TTAATCCACCTATAACTTCTCTGTTAACCCAA2235320.5426091867115269No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCC2143570.5203374793583146No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATT2080360.5049936687665265No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG2074920.5036731446466195No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1945350.4722208817392002No Hit
TTCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGG1879460.4562265188236344No Hit
ACGTACGCAGTTTTATCCGGTAAAGC1827360.4435795874546713No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATT1809650.43928060176284145No Hit
TTCAAAAAGTAAGTTTAGATTATAGCCAAAAGAG1781340.43240853598442797No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAA1741840.4228201715108379No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAA1722450.41811337689962497No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG1714720.41623697038365404No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG1569270.3809299422144471No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG1555710.37763834165085514No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCA1544280.3748637845386239No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAG1524830.37014242532314734No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1496100.36316840731488803No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC1480830.3594617155297812No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC1446620.35115746366543904No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCC1414350.3433241340056225No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCA1376730.3341921271322945No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC1349230.32751668351144064No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAGG1333680.3237420235731033No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG1322380.32099902310344336No Hit
TTCGACTGCATAATTTGTGGTAGTGGGG1271620.3086773678963691No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAG1232760.2992443592015917No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCA1138980.27647988273745816No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAA1123970.27283630423749394No Hit
ACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG1033960.2509869704079284No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGG1005040.24396683115283407No Hit
TTCAAAAAGTAAGTTTAGATTATAGCCAA990490.24043491461889138No Hit
CAAAAAGTAAGTTTAGATTATAGCCAA957040.23231514774188922No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC924430.2243992853245785No Hit
TTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG916320.22243063631493762No Hit
ACTATAAATAAATAAATAGAATTAAAATCCAACTT914960.22210050528496086No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC877450.21299520018611623No Hit
ACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGG834580.2025887904397161No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC825380.200355551119285No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACA746940.1813147584785659No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG746770.1812734920998188No Hit
TCGACTGCATAATTTGTGGTAGTGGG713250.17313673318450898No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAG695230.16876249703731674No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG686850.16672830730848928No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG682430.1656553814610648No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAG673350.16345127134916104No Hit
AACTATAAATAAATAAATAGAATTAAAATCCAACTT667690.16207734368028712No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAAAGA657770.15966932910869186No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAAAG643450.15619324355775996No Hit
TTCAAAAAGTAAGTTTAGATTATAGCCAAA629330.15276570668770703No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGC628380.15253510045353205No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAA617820.1499717301031242No Hit
TTTAAAGCATCTGGCCTACACCCAGAAGATT612310.14863421394490947No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAG607510.147469045603815No Hit
CCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG602580.1462723206201492No Hit
AAATGCCTAAAGGAAAGATCCAAAAAGATA594850.1443959141041783No Hit
ATTGATCATCGACACTTCGAACGCA591140.143495336073874No Hit
ATGCCTAAAGGAAAGATCCAAAAAGATA584120.1417912773750233No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAACA577010.1400653717697771No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGA575820.13977650711854742No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC567770.13782242271317022No Hit
TTAATCCACCTATAACTTCTCTGTTAACCCAACA564470.13702136947866775No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAG557920.1354314001798826No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGC555750.1349046469923461No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTG543350.13189462877785202No Hit
AAATGCCTAAAGGAAAGATCCAAAAAGAT536750.1302925223088471No Hit
TCTATAAATAAATAAATAGAATTAAAATCCAACTT530600.12879965037181978No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGATT525430.12754466697109926No Hit
AAAACATCACCTCTAGCATTACAAGTATTAGAGGC518780.1259304233318746No Hit
TTGGCAGGGGAGATACCATGATCACGAAGGTGGT498590.12102943399714591No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAAA492990.11967007093253569No Hit
TCGGAATGCCTAAAGGAAAGATCCAAAAAGATA492960.11966278863040385No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG484540.1176188891654006No Hit
ATCCACCTATAACTTCTCTGTTAACCCA479970.11650955180731688No Hit
TCAACTATAAATAAATAAATAGAATTAAAATCCAACTT473210.11486860639360881No Hit
ACCGGCTCCGGGACGGCCGGGAAGGCCCG467110.11338787162680124No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAG456200.11073954108485524No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGC456050.11070312957419604No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATT455940.11067642779971261No Hit
TTAATCCACCTATAACTTCTCTGTTAACCCAAC451780.10966661523743072No Hit
TCGGGAAACTCGACTGCATAATTTGTGGTAGTGGGG442980.1075304732787575No Hit
ATGCCTAAAGGAAAGATCCAAAAAGATAA442550.10742609361486777No Hit
AAATGCCTAAAGGAAAGATCCAAAAAGATAA441360.1071372289636381No Hit
TTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGG437110.1061055694949607No Hit
CCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGG432870.10507633746032724No Hit
TCTTTCGCCGAATCCCGGGGCCGAGGAAGCC431600.10476805333674598No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC423780.10286979991437956No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAAC423130.10271201670152301No Hit
ACCGGCTCCGGGACGGCCGGGAAGGCCC417590.10136721824117645No Hit
AAAAGGTTTGGTCCTGGCCTTATAATTAATTAGAGG414880.10070938361526685No Hit
ACGTACGCAGTTTTATCCGGTAAAG413410.10035255081080666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)