FastQCFastQC Report
Tue 18 Jul 2023
SRR10669505_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10669505_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40040926
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length18-135
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTAGGGATAACAGGGTAATGCGA7798361.9475973158063327No Hit
TCGACTGCATAATTTGTGGTAGTGGGG7390651.8457739963356492No Hit
TTAATCCACCTATAACTTCTCTGTTAACCCA5523531.3794710941500203No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG4011631.0018824239978865No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT3526090.880621492120337No Hit
TCGACTGCATAATTTGTGGTAGTGGG3338280.8337169824693864No Hit
TCGGGAAACTCGACTGCATAATTTGTGGTAGTGGGG2518090.6288790623873184No Hit
ACGTACGCAGTTTTATCCGGTAAAGC2065020.515727333578649No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCC2060820.5146784067880948No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG1845280.46084848287474667No Hit
TCGGGAAACTCGACTGCATAATTTGTGGTAGTGGG1713790.4280095819961806No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATT1679440.4194308593162905No Hit
TCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG1679400.4194208695373329No Hit
TTCGACTGCATAATTTGTGGTAGTGGGG1492120.37264872445757125No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1458670.3642947718042285No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCA1382210.34519930932666243No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAA1323970.3306541911643102No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1268020.31668098784728405No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATT1200030.29970086106400234No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1197500.29906900754493043No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC1148090.28672913308748155No Hit
CAAAAAGTAAGTTTAGATTATAGCCAA1121160.28000351440423726No Hit
AAATCCACCTATAACTTCTCTGTTAACCCA1068800.2669268937486611No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGC978880.24446987065184256No Hit
ACGTACGCAGTTTTATCCGGTAAAGCG978690.24442241920179367No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG973920.2432311380610928No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT968500.24187752301232995No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAG954990.23850347516938042No Hit
TTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGC945620.2361633694485487No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG936900.23398559763577895No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC898630.22442787661803829No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC884340.2208590280854144No Hit
ATGCCTAAAGGAAAGATCCAAAAAGATA881270.22009231255041406No Hit
TTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCA880800.21997493264766152No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAAAGAG865320.21610888819104732No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG849920.21226282329234844No Hit
TTCGACTGCATAATTTGTGGTAGTGGG843310.2106120123195952No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAG817630.20419857422877782No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC800890.2000178517349973No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCG775320.19363188553631352No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGG760510.1899331698772401No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC743950.18579740138876907No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGG733400.18316259718868638No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT725180.18110969761288737No Hit
ATGTTAGGGATAACAGGGTAATGCG720960.1800557759328543No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCA703700.17574518631262423No Hit
ATGAGGCGCTGCATGTGGCAGTCTGCCTTTCTTT690560.1724635439250331No Hit
TCAAAAAGTAAGTTTAGATTATAGCCAAA687080.1715944331557167No Hit
AAATGCCTAAAGGAAAGATCCAAAAAGATA659980.16482635791190242No Hit
TTTAATCAGTGAAATTGACCTTTCAGTGA646590.16148227940582593No Hit
TTTAATCAGTGAAATTGACCTTTCAGTG635510.15871511063455426No Hit
TCGACTGCATAATTTGTGGTAGTGG632620.15799334910486335No Hit
TTAATCCACCTATAACTTCTCTGTTAACCC623230.1556482484945528No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCC621900.15531608834421062No Hit
ACGTACGCAGTTTTATCCGGTAAAG613220.1531483063103985No Hit
TTCAAAAAGTAAGTTTAGATTATAGCCAA613030.15310085486034963No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAG610780.1525389297939813No Hit
CGACTGCATAATTTGTGGTAGTGGGG609480.15221426197785734No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG595950.14883521924542903No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAA591920.14782874901544485No Hit
TATGTTAGGGATAACAGGGTAATGCGA591310.14767640488634054No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCC580590.14499914412568782No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGG578080.14437228549609468No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC568050.14186734842246154No Hit
CCGTCAGCTCACATAGTGACGCAGGCAGTGCGACC552570.13800130396584734No Hit
ATTGATCATCGACACTTCGAACGCA538470.13447990688327238No Hit
TCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT534920.1335933140007801No Hit
ATGCCTAAAGGAAAGATCCAAAAAGAT534260.1334284826479787No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAG527300.13169026110934598No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAG525040.1311258385982382No Hit
TTAATCCACCTATAACTTCTCTGTTAACCCAA519070.12963486408880753No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT512930.1281014330188068No Hit
TTGGCAGGGGAGATACCATGATCACGAAGGTGGT509970.12736218937594No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGC509850.12733222003906702No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAGG508930.12710245512304086No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCG505060.1261359440088873No Hit
ATGCCTAAAGGAAAGATCCAAAAAGATAA496030.12388075140919569No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGC492830.12308156909258293No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC485150.12116353153271231No Hit
ATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCA483500.12075145315070886No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACA478550.1195152180046985No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG461910.11535946995831214No Hit
TCTACTAAAAGTATTGGAGAAAGAAATTCGTACA460150.11491991968417513No Hit
AAATGCCTAAAGGAAAGATCCAAAAAGAT458730.11456528253117823No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCA455490.11375611043560781No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGG451470.11275213765036304No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAAT431330.10772228394518148No Hit
TAATCCACCTATAACTTCTCTGTTAACCCA427460.10675577283102793No Hit
ACTATAAATAAATAAATAGAATTAAAATCCAACTT421620.10529726510320966No Hit
TCTCGACTGCATAATTTGTGGTAGTGGG413640.10330430420115659No Hit
TCAAAAAGTAAGTTTAGATTATAGCCA406840.10160604177835447No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGG401880.1003673091876047No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCC401310.10022495483745805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)