FastQCFastQC Report
Thu 29 Jun 2023
SRR1067765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1067765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30562326
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG17348285.676361151307659No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT14562184.764748599304909No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9647343.156611836415854Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC9496633.1072994902285904No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTA4961051.6232566853713948No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG4938461.6158652322470484No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC3719701.2170866837818561No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT2706400.8855346939234926No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC2693560.8813334430108493No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT2363620.7733770001668067No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2003300.6554802144313231No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT1787600.5849031255016388No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1679490.5495295089778179No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1592050.5209191211428083No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA1499030.49048295604202374No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1454630.47595526597026677Illumina Single End Adapter 1 (95% over 22bp)
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGC1302870.42629935954482No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT1176160.3848398188017496No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC1172480.3836357219669733No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1130480.3698933124396357No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG1013210.33152254183794777No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGC986690.32284519182211463No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC977370.31979568570795297No Hit
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA945180.3092631104059292Illumina Single End Adapter 1 (95% over 22bp)
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATTCGTATGCCGT873870.28593046222987084No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA787400.2576374586148973Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT780230.25529143298844464Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA730360.2389739576758654No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG670570.2194106561130197No Hit
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG662890.2168977583708779Illumina Single End Adapter 1 (95% over 22bp)
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT648140.21207155502496766No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATGCC630500.20629974302348586No Hit
TCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCG601840.19692218452221208No Hit
TGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGCC565680.18509062431962803No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC554390.1813965337585889Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG551540.180464013112091Illumina Single End Adapter 1 (95% over 22bp)
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGC534640.1749343292784718No Hit
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATG506430.16570401087927666No Hit
AAGGCTGAGGCGTGATGACGAGGCTGTAGGCACCATCAATTCGTATGCCG492470.1611362957125711No Hit
GAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG482600.15790682947364673No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGCCGTCTTCT459350.1502994241995848No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT440240.14404662786464617No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT438080.1433398753746688No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG430960.14101020975955822No Hit
CTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGC402040.1315475791993057No Hit
AAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATG393500.12875328926208038No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCCTGTAGGCACCATCAATTCGTA392300.12836064898987073No Hit
CCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATG390360.12772588054979847No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA361460.11826979399408277Illumina Single End Adapter 1 (95% over 22bp)
AGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATGC360630.11799821780580444No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC354990.11615280852641909No Hit
CAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC351980.11516793584362656No Hit
AAGGCTGAGGCGTGATGACCTGTAGGCACCATCAATTCGTATGCCGTCTT336660.11015522836841674No Hit
CTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA317130.10376500793820469Illumina Single End Adapter 1 (95% over 22bp)
GCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGCCG313460.10256418310569686No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATTCGTATGCCGT307370.1005715337242329No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)