FastQCFastQC Report
Wed 28 Jun 2023
SRR1067766.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1067766.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163797786
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG63076213.8508585213721997No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT55549553.3913492579197624No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA34964202.1345953967900395Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC34405182.1004667303622773No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTA19436591.186621045048802No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG18835921.1499496092090036No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC15232700.9299698348792089No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC13424290.8195648016878567No Hit
GCCAAGAATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATATC11271900.6881594846465141No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT11077040.6762631089531332No Hit
TGCCAAGAATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAT10309650.6294132693588422No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC10189840.6220987626780254No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT9074730.5540203089191938No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT7864380.4801273687545447No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT6950360.42432563771039No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT6642080.4055048704992874No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT6348290.38756873062985114No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA5900670.360241132929599No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5325770.3251429784282921Illumina Single End Adapter 1 (95% over 22bp)
CGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACCATCAATATCT5232320.31943777310885024No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGC5154120.31466359380461956No Hit
GCCAAGAATGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATATCT4747540.2898415244757948No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT4665730.2848469514722256No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT4524560.2762283978612507No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC4418470.2697515093396928No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC4041870.24675974558044392No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG3993710.24381953489896377No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGC3902670.2382614622153684No Hit
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA3788720.2313047137279377Illumina Single End Adapter 1 (95% over 22bp)
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT3773670.23038589788997513No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT3744910.22863007440161615Illumina Single End Adapter 1 (95% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA3654150.2230890959661689No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3477330.21229407826061825No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATTCGTATGCCGT3252210.19855030274951332No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATAT3194010.19499714117014988No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2878200.1757166607856348Illumina Single End Adapter 1 (95% over 22bp)
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG2866030.17497367150005314Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA2826380.17255300386050396No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG2557610.15614435716487646No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATGCC2502790.15279754758101555No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT2411790.1472419169328699No Hit
TCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCG2398370.146422614039484No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATATC2395450.1462443454516534No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC2346870.14327849339795107Illumina Single End Adapter 1 (95% over 22bp)
TGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGCC2136030.13040652454240131No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG2063160.1259577464618478Illumina Single End Adapter 1 (95% over 22bp)
TTGCCAAGAATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA2027820.12380020814200748No Hit
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATG1983600.12110053795232616No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATATC1970120.12027757200576568No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGC1946530.11883738159928485No Hit
GAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG1932350.11797168003235405No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGCCGTCTTCT1853510.11315842816092764No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT1757780.10731402682085092No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATCT1741610.10632683399029583No Hit
AAGGCTGAGGCGTGATGACGAGGCTGTAGGCACCATCAATTCGTATGCCG1731570.10571388309241249No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA1700490.10381642154796891Illumina Single End Adapter 1 (95% over 22bp)
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT1699600.10376208625921231No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGCTGTAGGCACCATCAA1682100.10269369574995355No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG1674440.10222604596132942No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)