FastQCFastQC Report
Thu 29 Jun 2023
SRR1067767.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1067767.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60601845
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG34208155.6447373838205745No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT26076044.3028458952033555No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA21652723.5729473252835784Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC18477643.049022682395231No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTA9963971.644169414314036No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG9471311.5628748596680513No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC7072091.1669760219346457No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT5118880.8446739534085143No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC5066900.836096656793205No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT4085130.6740933382473752No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT3613100.5962029703881128No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3230930.5331405339226883Illumina Single End Adapter 1 (95% over 22bp)
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT3129790.5164512730594258No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT3037260.5011827610198997No Hit
TATTCCTCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2935560.484401093729077No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2827670.46659800539075996No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGC2392160.3947338567002375No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA2305490.3804323119205364No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC2149310.35466081931993987No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT2071660.3418476780698673No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2025640.3342538498621618No Hit
CTGTCGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1983700.32733326848382255Illumina Single End Adapter 1 (95% over 22bp)
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGC1818140.3000139682215946No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1792180.2957302702582735Illumina Single End Adapter 1 (95% over 22bp)
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA1792110.29571871945482847Illumina Single End Adapter 1 (95% over 22bp)
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC1758760.2902155866706699No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATTCGTATGCCGT1467690.2421856958315378No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG1413110.23317936937398526No Hit
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG1350770.22289255384881435Illumina Single End Adapter 1 (95% over 22bp)
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG1292220.21323113182445189No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA1193620.1969610001147655No Hit
TCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCG1179010.194550182424314No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATGCC1131550.18671873768859676No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT1082670.17865297665442365No Hit
TGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGCC1028930.1697852598382112No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT980790.1618416072975996Illumina Single End Adapter 1 (95% over 22bp)
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATG944010.15577248514463546No Hit
GAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG921120.15199537241811698No Hit
AAGGCTGAGGCGTGATGACGAGGCTGTAGGCACCATCAATTCGTATGCCG907570.14975946689411848No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG876020.1445533547699744Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC872770.14401706746717036Illumina Single End Adapter 1 (95% over 22bp)
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGC868750.14335372132647115No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG807070.13317581337663895No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT800280.13205538544247292No Hit
CTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGC771300.12727335281623853No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT766160.12642519382041917No Hit
CCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATG755810.12471732502533546No Hit
AAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATG748280.12347478859760788No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCCTGTAGGCACCATCAATTCGTA726300.11984783631587455No Hit
AGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATGC684190.11289920298631173No Hit
CAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC668510.11031182301462934No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGCCGTCTTCT650000.10725746056081296No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC643070.1061139310197569No Hit
AAGGCTGAGGCGTGATGACCTGTAGGCACCATCAATTCGTATGCCGTCTT633120.10447206681578755No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)