FastQCFastQC Report
Sun 2 Jul 2023
SRR1067768.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1067768.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141202340
Total Bases7.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG70987845.027384107090577No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT68643424.861351447858442No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA46714053.3083056555578327Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC38518372.727884679531515No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCGTA25094861.7772269213102276No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG21689651.5360687365379355No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC15081471.0680750758096502No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT14195731.005346653603616No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC11703440.8288417883159727No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT11025890.7808574560449919No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT10166880.7200220619573302No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTAT8453190.5986579259238906No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT8297440.587627655462367No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA7677760.5437416971985025No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT7643310.5413019359310901No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7129140.5048882334386243Illumina Single End Adapter 1 (95% over 22bp)
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGC6228550.44110812894460527No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT5932190.4201198082128101No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT5673950.40183115945528947No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATGC5094980.3608282978879812No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC5036180.35666406094969816No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG4927490.3489665964459229No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGC4723920.33454969655602024No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATTCGTATGCCGT3908610.2768091520296335No Hit
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA3898160.2760690793084591Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3742900.2650735108214212Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATTCGTA3599240.2548994584650651No Hit
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG3105670.21994465530812027Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT3100010.21954381209263246Illumina Single End Adapter 1 (95% over 22bp)
TCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATTCG3064040.2169964038839583No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG3054310.21630732181917098No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATT2950210.20893492274986378No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATGCC2925260.2071679548653372No Hit
TGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGCC2785230.19725098040159955No Hit
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATG2402770.1701650270101756No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGC2392520.1694391183602198No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC2388780.16917425022843105Illumina Single End Adapter 1 (95% over 22bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGCCGTCTTCT2281190.16155468811635842No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATT2271270.16085215018391338No Hit
CTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGC2118080.1500031798339886No Hit
GAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATG2087140.14781199801646347No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCG2059980.1458885171449708No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATTCGT2049590.14515269364516198No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCCTGTAGGCACCATCAATTCGTA2000130.14164991883278988No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1963050.1390239000288522Illumina Single End Adapter 1 (95% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCTGTAGGCACCATCAATTCGTATGCCG1926560.13643966523500956No Hit
AAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATTCGTATG1880580.13318334526184197No Hit
CCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATG1872430.132606159359682No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC1765280.12501775820429037No Hit
AGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATTCGTATGC1758210.12451705828671113No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA1732450.12269272591374902Illumina Single End Adapter 1 (95% over 22bp)
CAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATTCGTATGCC1610410.11404980965612893No Hit
GCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATTCGTATGCCG1508310.10681905129900822No Hit
CTCTGAACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA1479050.10474684767971976Illumina Single End Adapter 1 (95% over 22bp)
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATTCGTATGCCGT1449510.1026548143607252No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)