FastQCFastQC Report
Thu 29 Jun 2023
SRR1067769.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1067769.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88792279
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT40783464.593131346476645No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA36159034.07231691845639Illumina PCR Primer Index 6 (96% over 29bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC12947831.4582157531962885No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT11916361.3420491211854129No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT9458151.0651995991678511No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA7140830.8042174477805666No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG6336360.7136161016883011No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG4364080.4914931848973039No Hit
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG2673000.3010396883720036No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTAT2203770.24819387730773304No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1893300.21322799924979965No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA1887570.21258267286956337No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA1874610.21112308650169911No Hit
ACTAACTGGAGGACCGAACCGACCTGTAGGCACCATCAATATCTCGTATG1863930.20992027921707018No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA1858170.20927157416468609No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC1399550.15762068681669947No Hit
AAGGCTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATATCTCGT1358120.1529547405805408No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT1291490.14545070974020163No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA1278040.14393593839392274No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA1268270.14283561749777815No Hit
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGT1156540.1302523161951953No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1098240.1236864299879047No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC1096160.12345217538565487No Hit
GAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTATGCCG1010670.11382408598837744No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA994810.11203789464622256No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATATCTCGTAT985370.11097473914370415No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT983640.11077990238317906No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT971640.10942843352404548No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTCTGTAGGCACCATCAATATCTC958590.10795871113973773No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC924770.1041498214050796No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATCTCGTATGC909240.10240079545655091No Hit
TTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTATGC904760.10189624708247438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)