FastQCFastQC Report
Wed 28 Jun 2023
SRR1067771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1067771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65430349
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT45195166.907369544979808No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA35592065.439686711742895Illumina PCR Primer Index 6 (96% over 29bp)
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT15550512.3766509330402625No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT12350211.887535400430158No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA7284591.1133350366203916No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG5733810.8763226985079966No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG4055600.619834688639671No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT2869150.43850446220300615No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTAT2409640.3682755841635508No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA2143260.3275635897953105No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA1938700.2962998103525323No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA1867500.28541800992074795No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1705780.2607016508501277No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT1569160.23982143210026283No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA1491100.22789118853698917No Hit
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG1432980.219008460431718No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA1145830.17512209815662147No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC1074690.16424946778138078No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA1001590.15307728222571454No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1001010.1529886383457927No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCA982820.15020858287031297No Hit
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGT982650.15018260104343933No Hit
GAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT932150.14246447011921026No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC911120.13925036530066498No Hit
ACTAACTGGAGGACCGAACCGACCTGTAGGCACCATCAATATCTCGTATG891630.13627162526673975No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA884270.13514676499738676No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATCTCGTATGC847090.12946438662584545No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT829580.1267882584578603No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATATCTC817490.12494049206431712No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC815610.12465316362594979No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC769670.11763195699903725No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTCTGTAGGCACCATCAATATCTC763900.1167501032280907No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCAT719740.11000094161197276No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC709890.10849552399605877No Hit
GAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTATGCCG687300.10504299770737888No Hit
AAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGT683540.10446834083064421No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATATCTCGTA681640.10417795570676232No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATATCTCGTAT670820.10252428884339285No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)