FastQCFastQC Report
Wed 28 Jun 2023
SRR1067772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1067772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70786149
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT50322217.109047562398119No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA28526904.030011577547466Illumina PCR Primer Index 6 (96% over 29bp)
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT16489592.3294938675078933No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT12926611.8261496327480677No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA7888411.114400219737904No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG6101340.8619398125472257No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG4356440.6154367855214161No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT2872960.40586471231822485No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA2195170.3101129290138386No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA1980210.27974540612457954No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTAT1967920.27800919075284064No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT1685620.23812850731574617No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1666100.2353709056838224No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA1657770.23419412179069102No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA1543870.2181034032519554No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC1426100.20146596758639887Illumina PCR Primer Index 6 (96% over 29bp)
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG1415710.19999816630793124No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT1401270.19795821919906956Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA1162060.16416488485621672No Hit
TAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGT1038410.1466967782072733No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC954140.13479190681781544No Hit
ACTAACTGGAGGACCGAACCGACCTGTAGGCACCATCAATATCTCGTATG937400.13242703738552017No Hit
GAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT922880.13037578863062604No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC912370.12889103488310968No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATATCTC907920.1282623808225533No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA873550.12340691114585142No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC871980.12318511634246412No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT854770.12075384973972804No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC826920.11681946421467283No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTCTGTAGGCACCATCAATATCTC794700.11226772627509374No Hit
AGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATCTCGTATGC771740.10902415386377354No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT729680.10308231346220008No Hit
AAAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGT727000.10270370832011218No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)