FastQCFastQC Report
Sat 30 Sept 2023
SRR10759495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10759495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81077173
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length10-40
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCCGAGTTGTAATTT16778432.069439446291498No Hit
TTGCCCGAGTTGTAATTTG13146761.6215118896659113No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG6450470.7955963141438096No Hit
TTTTAAAACCTAAGGGTAAACCTTTA6278240.7743535902515989No Hit
ACGTTCTAGCATTCAAGGTC4407780.5436524038646487No Hit
TTTTTAAAACCTAAGGGTAAACCTTTA3864150.4766014720320848No Hit
AGAGGTGCACAATCGACCGATCCTG2990010.36878567534662315No Hit
ATAAATCATTTGTATACGACTTAGAT2767080.34128965004736905No Hit
ATGTATTTATTAGAT2335570.28806751809168285No Hit
TGTGCCCGAGTTGTAATTT2191680.2703202293449477No Hit
ATATAAAGAATGGTACAGCAATTAATATA2154030.2656765055190072No Hit
TGTTAATAGATAATATACGGATCTTAATA2053960.2533339439449868No Hit
TCACGTTCTAGCATTCAAGGTC1916470.23637602657902243No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA1513540.18667893119559065No Hit
AATACTGAATATCTAAATATTATTATTACTT1485700.18324516568923782No Hit
TGTGCCCGAGTTGTAATTTG1459860.18005807873937588No Hit
GTGCCCGAGTTGTAATTT1302780.16068394491258348No Hit
TTAAGGCAAAAGATTGGAAATCTTTTG1250840.15427770280051575No Hit
TGTACACTGACTGTG1225240.15112021727743272No Hit
GACTCCATGGCCAAGTTGG1213690.14969564861369797No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT1201730.1482205108458826No Hit
AATACTGAATATCTAAATATTATTATTACT1194290.1473028666157366No Hit
TGCCCGAGTTGTAATTT1109010.13678449296696618No Hit
CTGCCCGAGTTGTAATTT1102190.13594331908933233No Hit
ATTAAATCAGTTATCGTTTATTTGATA1083770.1336714095840515No Hit
ATTTTAAAACCTAAGGGTAAACCTTTA955840.11789261571811341No Hit
CTGCCCGAGTTGTAATTTG882720.10887404769280744No Hit
AAGAGGTGCACAATCGACCGATCCTG824090.10164266580927779No Hit
TTAAATCAGTTATCGTTTATTTGATA821030.10126524761784678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)