FastQCFastQC Report
Sat 30 Sept 2023
SRR10759496.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10759496.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34873591
Total Bases944.4 Mbp
Sequences flagged as poor quality0
Sequence length10-40
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCCGAGTTGTAATTTG7605552.1808909785057695No Hit
ACGTTCTAGCATTCAAGGTC5429411.5568829719887465No Hit
TCACGTTCTAGCATTCAAGGTC4633091.3285382626641462No Hit
TTGCCCGAGTTGTAATTT3698471.06053603714054No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG3406570.9768337307161743No Hit
AAGAGGTGCACAATCGACCGATCCTG1840190.5276743653958664No Hit
TGTGCCCGAGTTGTAATTTG1813770.5200984320771554No Hit
TTTTAAAACCTAAGGGTAAACCTTTA1675250.48037783089214986No Hit
TTCACGTTCTAGCATTCAAGGTC1666070.4777454664763373No Hit
AGAGGTGCACAATCGACCGATCCTG1649250.47292233254671134No Hit
ATGTATTTATTAGATA1451910.4163351001048329No Hit
ATATAAAGAATGGTACAGCAATTAATATA1270230.3642383716663994No Hit
TGTTAATAGATAATATACGGATCTTAATA1264180.3625035345514031No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT1232550.3534336340642407No Hit
TTGTTAATAGATAATATACGGATCTTAATA1228050.35214325935060714No Hit
ATAAATCATTTGTATACGACTTAGAT1193660.3422819290390829No Hit
TGTACACTGACTGTG1169840.33545154555491574No Hit
TTTTTAAAACCTAAGGGTAAACCTTTA1099300.31522420504386833No Hit
AATACTGAATATCTAAATATTATTATTACTT1072770.3076167292321573No Hit
TGTGCCCGAGTTGTAATTT1013300.29056371051664853No Hit
ATATAAAGAATGGTACAGCAATTAATATAT872880.25029828445255325No Hit
ATTTTAAAACCTAAGGGTAAACCTTTA830430.238125749653943No Hit
TCACGTTCTAGCATTCAAGGTCCC769890.22076590850652578No Hit
CTGCCCGAGTTGTAATTTG761900.21847477651498523No Hit
ATGTATTTATTAGAT720200.20651730416864728No Hit
TCACGTTCTAGCATTCAAGGTCC695180.1993428207608445No Hit
GTGCCCGAGTTGTAATTTG664740.19061415269795418No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGG630390.18076429238388442No Hit
AATACTGAATATCTAAATATTATTATTACT616180.1766895757881659No Hit
TATTATCATAGTAGATAAGCTAAGTTGATA566330.16239509146046932No Hit
TATATAAAGAATGGTACAGCAATTAATATA532740.15276316109803545No Hit
TGCCCGAGTTGTAATTTG456800.1309873709306277No Hit
ACGGAGTCGAGCACCCGCAACGCGACTG435560.12489680228227715No Hit
CGGAGTCGAGCACCCGCAACGCGACTG416040.119299443524471No Hit
GTGCCCGAGTTGTAATTT409710.11748431642729308No Hit
TGTACACGGAGTCGAGCACCCGCAACGCGACTG408370.11710007151256663No Hit
AAGAGGTGCACAATCGACCGATCCTGA401240.1150555444662983No Hit
ACGTTCTAGCATTCAAGGTCC387030.11098082787057977No Hit
TACACGGAGTCGAGCACCCGCAACGCGACTG384480.11024961553285408No Hit
CTGCCCGAGTTGTAATTT377250.10817641349294943No Hit
TTTGCCCGAGTTGTAATTTG375670.10772334859349586No Hit
TGGAGTCGAGCACCCGCAACGCGACTG371150.106427238881135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)