FastQCFastQC Report
Sat 30 Sept 2023
SRR10759497.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10759497.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82959434
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length10-40
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCCGAGTTGTAATTT19079432.2998505510536633No Hit
TTGCCCGAGTTGTAATTTG14241011.7166233318322783No Hit
ACGTTCTAGCATTCAAGGTC6469670.7798594672186409No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG4223370.5090885745435534No Hit
TTTTAAAACCTAAGGGTAAACCTTTA4196500.5058496421275005No Hit
TCACGTTCTAGCATTCAAGGTC3423830.4127113499834148No Hit
TGTGCCCGAGTTGTAATTT3365750.40571033789839983No Hit
TTTTTAAAACCTAAGGGTAAACCTTTA2867620.34566532843027836No Hit
AGAGGTGCACAATCGACCGATCCTG2246140.27075160614041793No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA2210910.2665049522878857No Hit
ATAAATCATTTGTATACGACTTAGAT2197420.2648788563335666No Hit
TGTACACTGACTGTG2196320.26474626140771407No Hit
TGTGCCCGAGTTGTAATTTG2163920.2608407381371478No Hit
TGTTAATAGATAATATACGGATCTTAATA2014910.2428789473177939No Hit
GTGCCCGAGTTGTAATTT1907550.22993768255458444No Hit
CTGCCCGAGTTGTAATTT1859900.2241939114483351No Hit
AATACTGAATATCTAAATATTATTATTACTT1833470.22100801700262324No Hit
ATATAAAGAATGGTACAGCAATTAATATA1786410.21533536499296754No Hit
AATACTGAATATCTAAATATTATTATTACT1631280.19663586422250662No Hit
CTGCCCGAGTTGTAATTTG1409690.16992521911371766No Hit
TGCCCGAGTTGTAATTT1245870.15017821842902157No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT1222120.14731537343902323No Hit
GTGCCCGAGTTGTAATTTG1183930.14271191869510585No Hit
GACTCCATGGCCAAGTTGG1174690.1415981213179444No Hit
TTCACGTTCTAGCATTCAAGGTC1119540.13495029389906396No Hit
AAGAGGTGCACAATCGACCGATCCTG1061130.12790950333629325No Hit
TTGTTAATAGATAATATACGGATCTTAATA1048280.12636055352065204No Hit
TTAAGGCAAAAGATTGGAAATCTTTTG994950.11993211043363676No Hit
TGCCCGAGTTGTAATTTG954750.11508636859793425No Hit
ATTTTAAAACCTAAGGGTAAACCTTTA912670.11001400997986557No Hit
CCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCA898090.1082565245081108No Hit
ATGTATTTATTAGAT882070.10632546022433084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)