FastQCFastQC Report
Sat 30 Sept 2023
SRR10759499.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10759499.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62593663
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length10-40
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCCGAGTTGTAATTTG6057110.9676874158970373No Hit
TTGCCCGAGTTGTAATTT4754800.7596296129849438No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG3552290.5675159161079932No Hit
ACGTTCTAGCATTCAAGGTC3148990.5030844735832124No Hit
TCACGTTCTAGCATTCAAGGTC2481380.3964267117583452No Hit
TTTTAAAACCTAAGGGTAAACCTTTA2147660.3431114104953404No Hit
AATACTGAATATCTAAATATTATTATTACTT2068010.3303864801777138No Hit
AGAGGTGCACAATCGACCGATCCTG1965980.31408610804579373No Hit
AAGAGGTGCACAATCGACCGATCCTG1482870.236904173510344No Hit
ATAAATCATTTGTATACGACTTAGAT1480760.2365670786833485No Hit
ATATAAAGAATGGTACAGCAATTAATATA1478310.2361756652586381No Hit
TTTTTAAAACCTAAGGGTAAACCTTTA1414200.22593341437774622No Hit
TGTTAATAGATAATATACGGATCTTAATA1350850.2158125815388053No Hit
AATACTGAATATCTAAATATTATTATTACT1344820.2148492252322731No Hit
TGTGCCCGAGTTGTAATTTG1225730.19582333757971634No Hit
TTTTAAAACCTAAGGGTAAACCTTTAT1174810.18768832876900013No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA1102780.17618077408251376No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT1102150.17608012491615965No Hit
TGTGCCCGAGTTGTAATTT959000.153210397672365No Hit
TTGTTAATAGATAATATACGGATCTTAATA923640.14756126351001378No Hit
TTAAGGCAAAAGATTGGAAATCTTTTG907180.1449316043382858No Hit
TTCACGTTCTAGCATTCAAGGTC874500.13971062853439334No Hit
ATTTTAAAACCTAAGGGTAAACCTTTA733650.11720835062808195No Hit
CCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCA652860.1043012932475289No Hit

[OK]Adapter Content

Adapter graph