FastQCFastQC Report
Sat 30 Sept 2023
SRR10759504.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10759504.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35341536
Total Bases970 Mbp
Sequences flagged as poor quality0
Sequence length10-40
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGTTCTAGCATTCAAGGTC5993911.6959958955943508No Hit
ACGTTCTAGCATTCAAGGTC4849211.3720993903603964No Hit
TTGCCCGAGTTGTAATTTG4817091.3630109342163284No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG3237050.9159335915677237No Hit
AATACTGAATATCTAAATATTATTATTACTT2365730.6693908267031744No Hit
TTCACGTTCTAGCATTCAAGGTC2268120.6417717667958743No Hit
AAGAGGTGCACAATCGACCGATCCTG2014870.5701138739414155No Hit
TGTGCCCGAGTTGTAATTTG1997610.5652301020532893No Hit
TTGCCCGAGTTGTAATTT1843410.5215987216854412No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT1539960.43573657919112513No Hit
TCACGTTCTAGCATTCAAGGTCCC1274250.3605530897129089No Hit
TTTTAAAACCTAAGGGTAAACCTTTA1194420.33796493734737504No Hit
ATAAATCATTTGTATACGACTTAGAT1183710.33493450878875214No Hit
AGAGGTGCACAATCGACCGATCCTG1159380.32805025791748266No Hit
TCACGTTCTAGCATTCAAGGTCC1068850.3024345065251267No Hit
ATATAAAGAATGGTACAGCAATTAATATA1060860.30017371061631276No Hit
ATGTATTTATTAGATA989040.27985201322319436No Hit
AATACTGAATATCTAAATATTATTATTACT977730.27665181275652534No Hit
ATATAAAGAATGGTACAGCAATTAATATAT955460.27035044543621417No Hit
TTGTTAATAGATAATATACGGATCTTAATA941010.2662617719841039No Hit
ATTTTAAAACCTAAGGGTAAACCTTTA940310.26606370475805013No Hit
TGTTAATAGATAATATACGGATCTTAATA881250.24935248994271217No Hit
TGTGCCCGAGTTGTAATTT867310.24540812261244108No Hit
ATGTATTTATTAGAT742850.21019176982007798No Hit
TTTTTAAAACCTAAGGGTAAACCTTTA685700.1940209955786868No Hit
AAGAGGTGCACAATCGACCGATCCTGA654040.18506269789745414No Hit
TATTATCATAGTAGATAAGCTAAGTTGATA569290.16108241588594224No Hit
ACGTTCTAGCATTCAAGGTCC565350.15996758035643951No Hit
GTGCCCGAGTTGTAATTTG540930.15305786369896318No Hit
TTCACGTTCTAGCATTCAAGGTCCC540540.15294751195873318No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGG538930.1524919573388095No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA536740.1518722898744412No Hit
ACGTTCTAGCATTCAAGGTCCC522210.1477609801679248No Hit
CTGCCCGAGTTGTAATTTG489520.138511240711213No Hit
TATATAAAGAATGGTACAGCAATTAATATA476110.13471683856638264No Hit
TCCACGTTCTAGCATTCAAGGTC459390.12998586139549792No Hit
TTCACGTTCTAGCATTCAAGGTCC428560.12126241485372906No Hit
TATTATCATAGTAGATAAGCTAAGTTGATAAT412750.11678892507671426No Hit
TATATAAAGAATGGTACAGCAATTAATATAT392510.1110619527119591No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC366880.10380986270658978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)