FastQCFastQC Report
Sat 30 Sept 2023
SRR10759505.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10759505.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109039758
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length10-40
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCCGAGTTGTAATTTG14402921.3208870107727129No Hit
TTGCCCGAGTTGTAATTT13800651.2656530290538612No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG6660770.6108570050201322No Hit
TCACGTTCTAGCATTCAAGGTC6199500.5685540864828406No Hit
ACGTTCTAGCATTCAAGGTC5547410.5087511291065044No Hit
TTTTAAAACCTAAGGGTAAACCTTTA4169470.38238070924552126No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA3610570.33112417582584874No Hit
TGTGCCCGAGTTGTAATTT3527940.32354620596278283No Hit
TGTGCCCGAGTTGTAATTTG3140060.2879738599566591No Hit
AATACTGAATATCTAAATATTATTATTACTT2985930.2738386488348589No Hit
AGAGGTGCACAATCGACCGATCCTG2906740.2665761602295559No Hit
TTTTTAAAACCTAAGGGTAAACCTTTA2554190.23424391679225848No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT2536830.23265183695657135No Hit
ATAAATCATTTGTATACGACTTAGAT2517510.23088000617169382No Hit
TGTTAATAGATAATATACGGATCTTAATA2394960.21964098636389123No Hit
ATATAAAGAATGGTACAGCAATTAATATA2384820.21871105033083438No Hit
TTCACGTTCTAGCATTCAAGGTC2123720.19476565602796No Hit
AATACTGAATATCTAAATATTATTATTACT2120480.19446851670378798No Hit
TTGTTAATAGATAATATACGGATCTTAATA1798370.16492791555902023No Hit
AAGAGGTGCACAATCGACCGATCCTG1791310.1642804453032627No Hit
GTGCCCGAGTTGTAATTT1673180.1534467822278182No Hit
CTGCCCGAGTTGTAATTTG1417560.13000395690533356No Hit
GTGCCCGAGTTGTAATTTG1401310.12851367480107576No Hit
CTGCCCGAGTTGTAATTT1352710.12405658493849556No Hit
CCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCA1344600.12331281953138597No Hit
GACTCCATGGCCAAGTTGG1190230.10915559808927676No Hit
ATTTTAAAACCTAAGGGTAAACCTTTA1177220.10796245530919099No Hit
TCACGTTCTAGCATTCAAGGTCCC1109810.1017803065923899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)