FastQCFastQC Report
Sat 30 Sept 2023
SRR10759506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10759506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45026511
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length10-40
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCCGAGTTGTAATTTG6259501.3901809980346913No Hit
ACGTTCTAGCATTCAAGGTC6100871.3549506423004882No Hit
TCACGTTCTAGCATTCAAGGTC6086401.351736980020504No Hit
TTGCCCGAGTTGTAATTT4716901.0475828340330433No Hit
TTTTAAAACCTAAGGGTAAACCTTTA2520460.5597724416177838No Hit
ATAAATCATTTGTATACGACTTAGAT2283110.5070590523880475No Hit
AATACTGAATATCTAAATATTATTATTACTT2147930.4770367395332941No Hit
TTCACGTTCTAGCATTCAAGGTC2112890.4692546575505262No Hit
AGAGGTGCACAATCGACCGATCCTG1933770.4294736494240027No Hit
AAGAGGTGCACAATCGACCGATCCTG1878630.4172275306874211No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT1841500.4089812777188088No Hit
TGTGCCCGAGTTGTAATTTG1793730.39837197245862555No Hit
ATATAAAGAATGGTACAGCAATTAATATA1613380.3583177919337343No Hit
TGTGCCCGAGTTGTAATTT1597510.3547932017206485No Hit
TTTTTAAAACCTAAGGGTAAACCTTTA1484430.3296791083812823No Hit
TGTTAATAGATAATATACGGATCTTAATA1344840.29867737253725923No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG1284440.28526305313773925No Hit
AATACTGAATATCTAAATATTATTATTACT1188870.2640377798759491No Hit
TCACGTTCTAGCATTCAAGGTCCC1167660.259327221689462No Hit
TTGTTAATAGATAATATACGGATCTTAATA1149720.25534290231814766No Hit
ATTTTAAAACCTAAGGGTAAACCTTTA1017180.22590691070867117No Hit
TCACGTTCTAGCATTCAAGGTCC978740.2173697180312283No Hit
ATATAAAGAATGGTACAGCAATTAATATAT909540.20200099448078487No Hit
GTGCCCGAGTTGTAATTT797490.17711565526362902No Hit
GTGCCCGAGTTGTAATTTG757360.16820312815265656No Hit
CTGCCCGAGTTGTAATTTG730140.1621578007676411No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA717140.15927061281741328No Hit
ACGTTCTAGCATTCAAGGTCC674280.14975177623689298No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA667620.14827264764085318No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGG630130.13994644177515775No Hit
ATGTATTTATTAGATA607550.13493161839699283No Hit
TATATAAAGAATGGTACAGCAATTAATATA592660.13162467773707803No Hit
CTGCCCGAGTTGTAATTT574850.12766923024526594No Hit
ACGTTCTAGCATTCAAGGTCCC568450.12624784540823072No Hit
TTAAGGCAAAAGATTGGAAATCTTTTG507690.1127535731116275No Hit
TTCACGTTCTAGCATTCAAGGTCCC506770.11254924904130369No Hit
AAGAGGTGCACAATCGACCGATCCTGA505240.11220944922869995No Hit
TATTATCATAGTAGATAAGCTAAGTTGATA502580.11161868615580718No Hit
AGAGGTGCACAATCGACCGATCCTGA470840.1045695057296356No Hit

[OK]Adapter Content

Adapter graph