FastQCFastQC Report
Thu 24 Aug 2023
SRR10803852.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10803852.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73525409
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2990291940.67018382719911RNA PCR Primer, Index 1 (100% over 31bp)
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT41561335.652648596623243RNA PCR Primer, Index 1 (100% over 30bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA14371881.9546820882016445No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC11331461.5411624571853793No Hit
AGTCTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC10892761.4814960090871443RNA PCR Primer, Index 1 (100% over 31bp)
AGCGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC6902780.9388291876077833RNA PCR Primer, Index 1 (100% over 31bp)
AGAGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC6590840.896403038030023RNA PCR Primer, Index 1 (100% over 31bp)
AGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4507700.6130805746350897RNA PCR Primer, Index 1 (100% over 32bp)
AGTATACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3787160.5150817998169857RNA PCR Primer, Index 1 (100% over 31bp)
AACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA3321200.45170778988798277RNA PCR Primer, Index 1 (100% over 23bp)
TGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3102370.421945289688902RNA PCR Primer, Index 1 (100% over 31bp)
GGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2949200.40111303563098843RNA PCR Primer, Index 1 (100% over 31bp)
GGCATTAACGCGAACTCGGCCTATGGAATTCTCGGGTGCCAAGGAACTCC2930220.3985316151046504RNA PCR Primer, Index 1 (100% over 27bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAA2787640.37913967945421423No Hit
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC2690070.3658694370540666RNA PCR Primer, Index 1 (100% over 26bp)
GGCATACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2308920.3140302150512349RNA PCR Primer, Index 1 (100% over 31bp)
ATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGG2186570.29738970918203256Illumina Small RNA Adapter 2 (100% over 21bp)
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA2152030.29269201345075146RNA PCR Primer, Index 1 (100% over 23bp)
CATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAA2075310.28225752542226595No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAG2014670.27401003644876015No Hit
AGACTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1899610.25836102455411025RNA PCR Primer, Index 1 (100% over 31bp)
AACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAAC1876970.2552818169294373RNA PCR Primer, Index 1 (100% over 24bp)
GGCCTTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA1643110.2234751254494892No Hit
ATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAG1614780.2196220356965304No Hit
GGCATTAACGCGAACTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1548540.21061290526109144RNA PCR Primer, Index 1 (100% over 30bp)
AGTCTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1531080.2082382159887067RNA PCR Primer, Index 1 (100% over 30bp)
GCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCC1425260.19384591250624664RNA PCR Primer, Index 1 (100% over 27bp)
TTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGA1402400.190736783252712RNA PCR Primer, Index 1 (100% over 22bp)
AGTGTACTCCGAAGAGGACTGGAATTTTCGGGTGCCAAGGAACTCCAGTC1364850.18562970523564173RNA PCR Primer, Index 1 (96% over 31bp)
GGCCTTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC1309900.17815609839042174No Hit
GGCATTAACGCGAACTCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1297700.1764968080626386RNA PCR Primer, Index 1 (100% over 29bp)
CGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACT1289280.17535162572166038RNA PCR Primer, Index 1 (100% over 25bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGGAATTCTCGGGTGCCAAGG1174520.15974341604818545Illumina Small RNA Adapter 2 (100% over 21bp)
GCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTC1130650.15377677123836198RNA PCR Primer, Index 1 (100% over 26bp)
CGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTC1128230.15344763332088368RNA PCR Primer, Index 1 (100% over 26bp)
CGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCC1115350.15169585795843718RNA PCR Primer, Index 1 (100% over 27bp)
GCTGAGAAGACGGTCGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1038840.14128993148477417RNA PCR Primer, Index 1 (100% over 30bp)
TTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGG1038170.14119880652415004Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAAC1024440.1393314248683744RNA PCR Primer, Index 1 (100% over 24bp)
ACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1021440.13892340265662448RNA PCR Primer, Index 1 (100% over 30bp)
TAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGA1019440.13865138784879116RNA PCR Primer, Index 1 (100% over 22bp)
AGCGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT977010.13288059370060765RNA PCR Primer, Index 1 (100% over 30bp)
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC956460.13008564155012045RNA PCR Primer, Index 1 (100% over 24bp)
GGCATTAACGCGAACTCGGCCTACATGGAATTCTCGGGTGCCAAGGAACT919210.1250193657542252RNA PCR Primer, Index 1 (100% over 25bp)
AGAGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT900820.12251818959619797RNA PCR Primer, Index 1 (100% over 30bp)
CATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAG875190.11903231983381418No Hit
ACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACT831210.11305071420955985RNA PCR Primer, Index 1 (100% over 25bp)
CGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTC829980.11288342510274237RNA PCR Primer, Index 1 (100% over 26bp)
GGACTTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA818140.11127309744036921No Hit
GGCATTAACGCGAACTCGGCCTACAATATGGAATTCTCGGGTGCCAAGGA816260.1110174035210059RNA PCR Primer, Index 1 (100% over 22bp)
AGTGAGGCCCTCGGATCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC810020.11016871732056602RNA PCR Primer, Index 1 (100% over 31bp)
ATTCACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGG804120.10936627363745777Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA753380.10246525796272687RNA PCR Primer, Index 1 (100% over 23bp)
CCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTC752890.10239861433480771RNA PCR Primer, Index 1 (100% over 26bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC743190.1010793425168162No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)