FastQCFastQC Report
Tue 18 Jul 2023
SRR10803855.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10803855.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35377027
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC16258584.595801676607817RNA PCR Primer, Index 2 (100% over 50bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC14551464.11325123504584No Hit
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC13325343.766664734150781RNA PCR Primer, Index 1 (100% over 31bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA12324833.4838512574841296No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA10241042.8948277649221343No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG5262771.4876235925647454No Hit
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT4877671.3787676392366155RNA PCR Primer, Index 1 (100% over 30bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG4062631.1483808404815927No Hit
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC3316850.9375717185053453RNA PCR Primer, Index 1 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2754580.7786352425827077RNA PCR Primer, Index 1 (100% over 24bp)
ACGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA2473910.6992984458530108No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG2338910.6611380882853723Illumina Small RNA Adapter 2 (100% over 21bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAA1982980.5605275988850053No Hit
CGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC1903980.5381967229750538RNA PCR Primer, Index 1 (100% over 27bp)
GAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGGAACT1689960.4776998361111577RNA PCR Primer, Index 1 (100% over 25bp)
CGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1552370.4388073650168512RNA PCR Primer, Index 1 (100% over 31bp)
TCGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTC1311470.37071232695726525RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG1248730.35297765411434945RNA PCR Primer, Index 1 (100% over 29bp)
GGCATTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAG1207580.34134581178910256No Hit
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1118740.3162334698164433RNA PCR Primer, Index 1 (100% over 27bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA1071080.3027614502484904RNA PCR Primer, Index 1 (100% over 22bp)
ACGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1063830.30071209771245055No Hit
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA1062200.3002513467284857RNA PCR Primer, Index 1 (100% over 23bp)
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA877320.24799144371289314No Hit
GCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA870310.2460099318125291No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGAA778880.2201654763132018RNA PCR Primer, Index 1 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGA754090.2131581039865221RNA PCR Primer, Index 1 (100% over 22bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGGAATTCTCGGGTGCCAAGGAA671850.18991137949494738RNA PCR Primer, Index 1 (100% over 23bp)
CGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAA620010.17525780218897422RNA PCR Primer, Index 1 (100% over 23bp)
AGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG616690.17431934006212563No Hit
CGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT606380.17140501942121933RNA PCR Primer, Index 1 (100% over 30bp)
CAGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA584260.1651523741664329No Hit
TTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGG563770.15936047989561136Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAA554720.156802322592003No Hit
ATAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC550340.1555642309909196RNA PCR Primer, Index 1 (100% over 24bp)
CGGGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC549100.15521372103992798RNA PCR Primer, Index 1 (100% over 31bp)
AGGGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC527580.14913067737433108RNA PCR Primer, Index 1 (100% over 31bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC472690.1336149586566446RNA PCR Primer, Index 2 (98% over 50bp)
CCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTC463370.13098048063790096RNA PCR Primer, Index 1 (100% over 26bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGGAATTCTCGGGTGCCAAGG456660.1290837695321317Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGGAATTCTCGGGTGCCAAGGA448070.1266556401135686RNA PCR Primer, Index 1 (100% over 22bp)
CTCGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACT446490.1262090225953696RNA PCR Primer, Index 1 (100% over 25bp)
CGTTTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC441950.12492570390383567RNA PCR Primer, Index 1 (100% over 31bp)
GCAGTTAAAAAGCTCGTAGTTGGATCTTGTGGAATTCTCGGGTGCCAAGG434120.12271240316491265Illumina Small RNA Adapter 2 (100% over 21bp)
AACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA424960.12012315223661955RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTACAATATGGAATTCTCGGGTGCCAAGGA420490.11885962039715774RNA PCR Primer, Index 1 (100% over 22bp)
CTAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC418760.11837060248166133RNA PCR Primer, Index 1 (100% over 24bp)
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC406630.11494182368688019RNA PCR Primer, Index 1 (100% over 26bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG399440.11290943130975929No Hit
AGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAACT395710.11185507476363121RNA PCR Primer, Index 1 (100% over 25bp)
GGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA383020.1082680011522732RNA PCR Primer, Index 1 (100% over 28bp)
TCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG377250.10663699920290079No Hit
GCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG371440.10499469048091577No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAAGG369460.1044350052365904Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC366460.10358699729064288RNA PCR Primer, Index 1 (100% over 27bp)
CGGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA361310.1021312503167663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)