FastQCFastQC Report
Thu 24 Aug 2023
SRR10803856.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10803856.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90310758
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC1269672614.058929723521976No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA40769534.5143602935986875No Hit
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC30234523.347831495335251RNA PCR Primer, Index 1 (100% over 31bp)
AACTTCTTAGAGGGACAAGTGGCGTGGAATTCTCGGGTGCCAAGGAACTC20580132.2788126747867623RNA PCR Primer, Index 1 (100% over 26bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGTG16081811.7807191918375882No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCTGGAATTCTCGGGTGCCAAGG14016751.5520576186504824Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC13598161.5057076588815699RNA PCR Primer, Index 1 (100% over 50bp)
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC12272111.3588757609586226RNA PCR Primer, Index 1 (100% over 27bp)
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT12142471.3445208819972476RNA PCR Primer, Index 1 (100% over 30bp)
GCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG10530771.1660593082387813RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTGGAATTCTCGGGTGCCA9272741.0267591818906006No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCTGGAATTCTCGGGTGCCAAGGA9099371.0075621334060778RNA PCR Primer, Index 1 (100% over 22bp)
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG8735330.9672524285534178Illumina Small RNA Adapter 2 (100% over 21bp)
AACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA7201080.7973667987594567RNA PCR Primer, Index 1 (100% over 23bp)
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG6853680.7588996207960075No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATATGGAATTCTCGGGTGC6419590.7108333649463999No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAA6242230.6911945086320724No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAA6230890.68993884427368No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTGGAATTCTCGGGTGCCAA5716190.6329467415166641No Hit
GCAGTTAAAAAGCTCGTAGTTGGATCTTGTGGAATTCTCGGGTGCCAAGG5715850.6329090937316681Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC5426540.6008741505635464No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCTGGAATTCTCGGGTGC5414040.5994900408210504No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAG5369110.5945149967626226No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT5258950.5823171144239538No Hit
AACTTCTTAGAGGGACAAGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC4843840.536352490807352RNA PCR Primer, Index 1 (100% over 27bp)
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT4796680.5311305215708632RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA4759890.5270568097767488RNA PCR Primer, Index 1 (100% over 23bp)
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA4688450.5191463457764356RNA PCR Primer, Index 1 (100% over 28bp)
AGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4677780.517964869700241RNA PCR Primer, Index 1 (100% over 32bp)
CTGGGTCGGGGTTTCGTACGTAGTGGAATTCTCGGGTGCCAAGGAACTCC4623840.5119921593394222RNA PCR Primer, Index 1 (100% over 27bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGTGGAATTCTCGGGTGCCAA4473910.495390593444028No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC4124070.4566532372588435No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGCC4114310.4555725243719026No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAG4068870.4505410086359811No Hit
CACAGGGATAACTGGCTTGTGGCGGCCAAGTGGAATTCTCGGGTGCCAAG3439260.3808250618381478No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA3422750.37899692969025905RNA PCR Primer, Index 1 (100% over 22bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGTGGAATTCTCGGGTGCCAAGGA3287760.36404965175909604RNA PCR Primer, Index 1 (100% over 22bp)
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA3245410.3593602879515196RNA PCR Primer, Index 1 (100% over 23bp)
GAAGCTACCATCTGTGGGATTATGACTGAACTGGAATTCTCGGGTGCCAA3125990.34613705711560966No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCC3006450.33290053882617177No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCCA2880250.31892656686593196No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGTGGAATTCTCGGGTGCCAAG2871960.31800862528470863No Hit
AACTTCTTAGAGGGACAAGTGGCGTTGGAATTCTCGGGTGCCAAGGAACT2867620.317528062382114RNA PCR Primer, Index 1 (100% over 25bp)
ATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAG2739300.30331934540954686No Hit
CTGGGTCGGGGTTTCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2698720.29882597154150786RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTCAGCGGTTACTTCGACTGGAATTCTCGGGTGCCAAGGAACTCCAG2665580.29515641979220236RNA PCR Primer, Index 1 (100% over 29bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT2651810.29363168449986876RNA PCR Primer, Index 1 (100% over 25bp)
TGGGTCGGGGTTTCGTACGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA2630790.2913041655568875RNA PCR Primer, Index 1 (100% over 28bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGTGGAATTCTCGGGTGCCAAGGAA2448790.2711515277061455RNA PCR Primer, Index 1 (100% over 23bp)
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA2356950.26098219660607874RNA PCR Primer, Index 1 (100% over 28bp)
CACAGGGATAACTGGCTTGTGGCGGCCAATGGAATTCTCGGGTGCCAAGG2345500.25971435208195237Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGAGGTAGTGACGAAAAATAACATGGAATTCTCGGGTGCCAAGGAAC2329110.25789950738759165RNA PCR Primer, Index 1 (100% over 24bp)
GCAGTTAAAAAGCTCGTAGTTGGATCTTTGGAATTCTCGGGTGCCAAGGA2247220.24883192764255171RNA PCR Primer, Index 1 (100% over 22bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC2237940.2478043645697227RNA PCR Primer, Index 1 (100% over 26bp)
ACGGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA2226270.2465121597141284RNA PCR Primer, Index 1 (100% over 22bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC2217080.24549456223144533RNA PCR Primer, Index 1 (100% over 27bp)
GAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGC2190780.24258239533323372No Hit
GCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAGG2097130.23221264514245357Illumina Small RNA Adapter 2 (100% over 21bp)
AACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAAC2089960.23141871979415785RNA PCR Primer, Index 1 (100% over 24bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACATGGAATTCTCGGGTGCCAAG2046900.2266507385532076No Hit
CGGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGGAA2043470.22627093883986668RNA PCR Primer, Index 1 (100% over 23bp)
GCTAACTAGTTACGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2039450.22582580914667993RNA PCR Primer, Index 1 (100% over 31bp)
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGGAATTCTCGGGTG1878310.2079829736342153No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGT1828840.2025052209173131No Hit
CGCCGCGACCGGCTCCGGGACGGCCGTGGAATTCTCGGGTGCCAAGGAAC1789700.19817129649160956RNA PCR Primer, Index 1 (100% over 24bp)
GACGTCGCTTTTTGATCCTTCGATTGGAATTCTCGGGTGCCAAGGAACTC1767130.19567214794055876RNA PCR Primer, Index 1 (100% over 26bp)
CCGTGGCGCAATGAAGGTGAAGGGTGGAATTCTCGGGTGCCAAGGAACTC1765280.19546729969866936RNA PCR Primer, Index 1 (100% over 26bp)
TAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGA1749370.19370560481842042RNA PCR Primer, Index 1 (100% over 22bp)
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC1716730.19009141745881483RNA PCR Primer, Index 1 (100% over 26bp)
GCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCA1676510.18563790595135962No Hit
CAACTTCTTAGAGGGACAAGTGGCGTGGAATTCTCGGGTGCCAAGGAACT1672420.18518502524361494RNA PCR Primer, Index 1 (100% over 25bp)
GCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTC1571140.17397041446601522RNA PCR Primer, Index 1 (100% over 26bp)
CATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAA1563940.1731731672543375No Hit
TTGATCATCGACACTTCGAACGCTGGAATTCTCGGGTGCCAAGGAACTCC1508260.16700778881736325RNA PCR Primer, Index 1 (100% over 27bp)
TTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGG1501330.16624043837612348Illumina Small RNA Adapter 2 (100% over 21bp)
GATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAGGAA1500720.16617289382068967RNA PCR Primer, Index 1 (100% over 23bp)
GCTAACTAGTTACGCGACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1446280.16014481907017103RNA PCR Primer, Index 1 (100% over 30bp)
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCTGGAATTCTCGGGTG1360010.15059224727136053No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1353770.1499012996879065RNA PCR Primer, Index 1 (100% over 24bp)
GGCATTAACGCGAACTCGGCCTACAATAGTCTGGAATTCTCGGGTGCCAA1294200.1433051862990675No Hit
TTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGTGGAATTCTCGGGTG1209750.1339541408787644No Hit
ATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAGGAAC1208600.13382680278245476RNA PCR Primer, Index 1 (100% over 24bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGGAATTCTCGGGTGCCAAGG1205950.1335333715170456Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGTTAAAAAGCTCGTAGTTGGATCTTGGAATTCTCGGGTGCCAAGGAA1185210.13123685663229623RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1182040.13088584640159925RNA PCR Primer, Index 1 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCTGGAATTCTCGGGTG1157910.12821396095468493No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGATGGAATTCTCGGGTGCCAAGGA1147660.12707899096583822RNA PCR Primer, Index 1 (100% over 22bp)
GGGGAGGTAGTGACGAAAAATAACATGGAATTCTCGGGTGCCAAGGAACT1112790.12321787842817132RNA PCR Primer, Index 1 (100% over 25bp)
GGCATTAACGCGAACTCGGCCTATGGAATTCTCGGGTGCCAAGGAACTCC1061850.11757735440555155RNA PCR Primer, Index 1 (100% over 27bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGGT1058770.11723630976500053No Hit
ACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAAC1008300.11164782826869862RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCTGGAATTCTCGGGTGCCAAGGAACTC1000450.11077860735041112RNA PCR Primer, Index 1 (100% over 26bp)
GAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCCA991120.10974550783861209RNA PCR Primer, Index 1 (100% over 28bp)
TGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGTGGAATTCTCGGGTGC978810.10838243656420203No Hit
TCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAGGAACT966230.10698946851935402RNA PCR Primer, Index 1 (100% over 25bp)
TAATCCACCTATAACTTCTCTGTTAACCCAATGGAATTCTCGGGTGCCAA966130.10697839564141405No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGTGGAATTCTCGGGTGCCAAGGAA965990.1069628936122981RNA PCR Primer, Index 1 (100% over 23bp)
AACTTCTTAGAGGGACAAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA945260.1046674860153427RNA PCR Primer, Index 1 (100% over 28bp)
GGCATTAACGCGAACTCGGCCTACAATATGGAATTCTCGGGTGCCAAGGA932430.10324683577564481RNA PCR Primer, Index 1 (100% over 22bp)
ATCTATTGAAAGTCAGCCCTCGTGGAATTCTCGGGTGCCAAGGAACTCCA920680.10194577261769854RNA PCR Primer, Index 1 (100% over 28bp)
CGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACT917440.10158701137244358RNA PCR Primer, Index 1 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCTGGAATTCTCGGGTGCCAAGGAACTC903180.10000801897820412RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)