FastQCFastQC Report
Thu 24 Aug 2023
SRR10803857.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10803857.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65687098
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC43398486.60684994791519RNA PCR Primer, Index 1 (100% over 31bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC38885225.919765248268389No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA20372833.1014964308516113No Hit
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT15245702.320958066986001RNA PCR Primer, Index 1 (100% over 30bp)
AACTTCTTAGAGGGACAAGTGGCGTGGAATTCTCGGGTGCCAAGGAACTC13241442.0158357429643186RNA PCR Primer, Index 1 (100% over 26bp)
GCAGTTAAAAAGCTCGTAGTTGGATCTTGTGGAATTCTCGGGTGCCAAGG12514701.905199100133789Illumina Small RNA Adapter 2 (100% over 21bp)
AACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA10550981.6062484599334865RNA PCR Primer, Index 1 (100% over 23bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCTGGAATTCTCGGGTGCCAAGG10339321.5740259982257094Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCTGGAATTCTCGGGTGCCAAGGA8714861.3267232478438917RNA PCR Primer, Index 1 (100% over 22bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCCA8620271.3123231597169966No Hit
GCAGTTAAAAAGCTCGTAGTTGGATCTTGGAATTCTCGGGTGCCAAGGAA7804681.1881602685507586RNA PCR Primer, Index 1 (100% over 23bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT7102261.0812260270654672RNA PCR Primer, Index 1 (100% over 25bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC6957861.0592430190781148RNA PCR Primer, Index 1 (100% over 24bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC6754291.0282521538704603No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTGGAATTCTCGGGTGCCAA6391980.97309520356646No Hit
GCAGTTAAAAAGCTCGTAGTTGGATCTTTGGAATTCTCGGGTGCCAAGGA6182830.9412548564712053RNA PCR Primer, Index 1 (100% over 22bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTGGAATTCTCGGGTGCCA5721880.8710812586057616No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGTGGAATTCTCGGGTGCCAAGGA5611910.8543397669965569RNA PCR Primer, Index 1 (100% over 22bp)
GAAGCTACCATCTGTGGGATTATGACTGAACTGGAATTCTCGGGTGCCAA5149220.7839012769296034No Hit
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC5030610.7658444585266958RNA PCR Primer, Index 1 (100% over 26bp)
CTGGGTCGGGGTTTCGTACGTAGTGGAATTCTCGGGTGCCAAGGAACTCC4935010.751290611133407RNA PCR Primer, Index 1 (100% over 27bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGTGGAATTCTCGGGTGCCAAGGAA4763420.7251682819052229RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAA4623220.7038246688870317No Hit
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC4511630.6868365535040077RNA PCR Primer, Index 1 (100% over 27bp)
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA3839600.58452879133129RNA PCR Primer, Index 1 (100% over 23bp)
ATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAG3571520.5437171238711139No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATATGGAATTCTCGGGTGC3308620.5036940435395699No Hit
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC3183750.4846842221588173RNA PCR Primer, Index 1 (100% over 26bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGTG3093770.47098594612902517No Hit
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT2944270.4482265299648342RNA PCR Primer, Index 1 (100% over 25bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGTGGAATTCTCGGGTGCCAA2908980.4428540898549057No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGCC2888450.4397286663508867No Hit
TGGGTCGGGGTTTCGTACGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA2872690.437329412847558RNA PCR Primer, Index 1 (100% over 28bp)
AACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAAC2844560.433046988923152RNA PCR Primer, Index 1 (100% over 24bp)
CTGGGTCGGGGTTTCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2800170.42628919304670765RNA PCR Primer, Index 1 (100% over 31bp)
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA2791080.4249053596491658RNA PCR Primer, Index 1 (100% over 28bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGTGGAATTCTCGGGTGCCAAG2789060.4245978411163787No Hit
GCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCC2662040.4052607104061744RNA PCR Primer, Index 1 (100% over 27bp)
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT2611550.3975742694554721No Hit
TTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGG2492260.3794139299623192Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC2408040.36659253846166256No Hit
GCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTC2386520.36331640042919844RNA PCR Primer, Index 1 (100% over 26bp)
GGCATTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAG2272650.3459811849200584No Hit
GGCATTAACGCGAACTCGGCCTATGGAATTCTCGGGTGCCAAGGAACTCC2226880.33901330212517533RNA PCR Primer, Index 1 (100% over 27bp)
TAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGA2161760.329099635365228RNA PCR Primer, Index 1 (100% over 22bp)
GCAGTTAAAAAGCTCGTAGTTGGATCTGGAATTCTCGGGTGCCAAGGAAC1875770.28556140507227157RNA PCR Primer, Index 1 (100% over 24bp)
CGGGGAGGTAGTGACGAAAAATAACATGGAATTCTCGGGTGCCAAGGAAC1831990.278896473703253RNA PCR Primer, Index 1 (100% over 24bp)
CATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAA1723400.262365069012487No Hit
AACTTCTTAGAGGGACAAGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC1703210.25929140605359063RNA PCR Primer, Index 1 (100% over 27bp)
GCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCA1626190.2475661202143532No Hit
CAACTTCTTAGAGGGACAAGTGGCGTGGAATTCTCGGGTGCCAAGGAACT1614380.2457682024558308RNA PCR Primer, Index 1 (100% over 25bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAA1584030.24114781261915394No Hit
AACTTCTTAGAGGGACAAGTGGCGTTGGAATTCTCGGGTGCCAAGGAACT1578460.24029985310052823RNA PCR Primer, Index 1 (100% over 25bp)
ATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGG1561400.23770269163055432Illumina Small RNA Adapter 2 (100% over 21bp)
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGGAATTCTCGGGTG1534710.23363948883843216No Hit
TTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGA1527590.2325555621288065RNA PCR Primer, Index 1 (100% over 22bp)
CGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACT1451000.2208957381554594RNA PCR Primer, Index 1 (100% over 25bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGGAATTCTCGGGTGCCAAGG1355110.20629774206191906Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAAC1282270.1952088064538945RNA PCR Primer, Index 1 (100% over 24bp)
TAGCTCAGCGGTTACTTCGACTGGAATTCTCGGGTGCCAAGGAACTCCAG1271930.19363467693457856RNA PCR Primer, Index 1 (100% over 29bp)
AACTTCTTAGAGGGACAAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA1250080.190308300725966RNA PCR Primer, Index 1 (100% over 28bp)
CATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1240440.18884073703484358RNA PCR Primer, Index 1 (100% over 30bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCATGGAATTCTCGGGTGCCAAG1234220.1878938235328953No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCATGGAATTCTCGGGTGCCAAGG1196140.1820966424791669Illumina Small RNA Adapter 2 (100% over 21bp)
GCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1193990.18176933314971533RNA PCR Primer, Index 1 (100% over 29bp)
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC1149290.17496434383507092RNA PCR Primer, Index 1 (100% over 27bp)
ACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1147900.17475273454765805RNA PCR Primer, Index 1 (100% over 30bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG1098750.1672702910394976No Hit
GGCATTAACGCGAACTCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1060830.16149746788935632RNA PCR Primer, Index 1 (100% over 29bp)
GGCATTAACGCGAACTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1042040.15863693658684694RNA PCR Primer, Index 1 (100% over 30bp)
ATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAGGAACTCCA1019690.15523444192952474RNA PCR Primer, Index 1 (100% over 28bp)
GGCATTAACGCGAACTCGGCCTACAATATGGAATTCTCGGGTGCCAAGGA1012770.1541809625993829RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGGAA1012020.1540667849263184RNA PCR Primer, Index 1 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGT992580.15110729964048647No Hit
GGCATTAACGCGAACTCGGCCTACATGGAATTCTCGGGTGCCAAGGAACT985240.14998988081342854RNA PCR Primer, Index 1 (100% over 25bp)
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGTGGAATTCTCGGGTGCCAAG972330.1480245024677449No Hit
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG966130.147080633703745No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA922470.14043397076241668RNA PCR Primer, Index 1 (100% over 22bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCTGGAATTCTCGGGTGC919310.13995290216657158No Hit
AGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA917590.13969105470301033RNA PCR Primer, Index 1 (100% over 32bp)
ATCTATTGAAAGTCAGCCCTCGTGGAATTCTCGGGTGCCAAGGAACTCCA913440.13905927157872008RNA PCR Primer, Index 1 (100% over 28bp)
TTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGTGGAATTCTCGGGTG905290.13781854086475248No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCTGGAATTCTCGGGTG895890.1363875140290107No Hit
GAAGCTACCATCTGTGGGATTATGACTGAACGTGGAATTCTCGGGTGCCA875190.13323621025243038No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA871330.13264857582839176RNA PCR Primer, Index 1 (100% over 23bp)
AACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAACT869160.13231822176099178RNA PCR Primer, Index 1 (100% over 25bp)
GAAGCTACCATCTGTGGGATTATGACTGAATGGAATTCTCGGGTGCCAAG865160.13170927417131445No Hit
GACGTCGCTTTTTGATCCTTCGATTGGAATTCTCGGGTGCCAAGGAACTC834480.12703864615848914RNA PCR Primer, Index 1 (100% over 26bp)
GGCATTAACGCGAACTCGGCCTACAATGGAATTCTCGGGTGCCAAGGAAC834130.12698536324439236RNA PCR Primer, Index 1 (100% over 24bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACATGGAATTCTCGGGTGCCAAG833790.12693360269926982No Hit
TGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGTGGAATTCTCGGGTGC818680.1246333031792636No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGTGGAATTCTCGGGTGCCAAGGAA791870.12055183195945116RNA PCR Primer, Index 1 (100% over 23bp)
CGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTC789750.12022908973692216RNA PCR Primer, Index 1 (100% over 26bp)
ACCAGGAGTGGAGCCTGCGGCTTAATTTGTGGAATTCTCGGGTGCCAAGG783770.11931871309035452Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCC781730.11900814981961906RNA PCR Primer, Index 1 (100% over 27bp)
CGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC761850.11598168029892263RNA PCR Primer, Index 1 (100% over 31bp)
AGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC759280.11559043147255493RNA PCR Primer, Index 1 (100% over 26bp)
ATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAGGAAC756170.11511697472158079RNA PCR Primer, Index 1 (100% over 24bp)
CTGGGTCGGGGTTTCGTACGTAGCTGGAATTCTCGGGTGCCAAGGAACTC749230.11406045065349057RNA PCR Primer, Index 1 (100% over 26bp)
CGCCGCGACCGGCTCCGGGACGGTGGAATTCTCGGGTGCCAAGGAACTCC738770.1124680527064843RNA PCR Primer, Index 1 (100% over 27bp)
CCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG737290.11224274209830369RNA PCR Primer, Index 1 (100% over 29bp)
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGGAATTCTCGGGTGCCAAGGA734530.11182256826142631RNA PCR Primer, Index 1 (100% over 22bp)
AGTTAAAAAGCTCGTAGTTGGATCTTGTGGAATTCTCGGGTGCCAAGGAA733580.11167794320887794RNA PCR Primer, Index 1 (100% over 23bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACTGGAATTCTCGGGTGCCAAGG730940.11127603779969089Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTAAAAAGCTCGTAGTTGGATCTTGGAATTCTCGGGTGCCAAGGAACT727830.11080258104871675RNA PCR Primer, Index 1 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCTGGAATTCTCGGGTG722570.11000181496829103No Hit
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG719170.10948420951706528Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCTGGAATTCTCGGGTGCCAAGGAACTC717410.10921627257760726RNA PCR Primer, Index 1 (100% over 26bp)
AACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAACTCCAG693360.10555497519467218RNA PCR Primer, Index 1 (100% over 29bp)
CGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCC691050.10520330796163349RNA PCR Primer, Index 1 (100% over 27bp)
ATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGTGCCAAGG679250.10340691257208531Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC668460.10176427644893066RNA PCR Primer, Index 2 (100% over 50bp)
AAAAAGCTCGTAGTTGGATCTTGTGGAATTCTCGGGTGCCAAGGAACTCC657970.1001673113950018RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)