FastQCFastQC Report
Thu 24 Aug 2023
SRR10803858.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10803858.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70365566
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTTCTTAGAGGGACAAGTGGCGTGGAATTCTCGGGTGCCAAGGAACTC29782454.2325318608252225RNA PCR Primer, Index 1 (100% over 26bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC25387493.607942271081853No Hit
GCAGTTAAAAAGCTCGTAGTTGGATCTTGTGGAATTCTCGGGTGCCAAGG22593773.2109128490489227Illumina Small RNA Adapter 2 (100% over 21bp)
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT22486213.1956269633360157No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCTGGAATTCTCGGGTGCCAAGG15429122.1927088598988886Illumina Small RNA Adapter 2 (100% over 21bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA13543261.9246999306450543No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCTGGAATTCTCGGGTGCCAAGGA11145091.5838840833029042RNA PCR Primer, Index 1 (100% over 22bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGTG11031491.5677398231970447No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATATGGAATTCTCGGGTGC9151221.3005253166015889No Hit
GCAGTTAAAAAGCTCGTAGTTGGATCTTGGAATTCTCGGGTGCCAAGGAA9036741.2842559953258956RNA PCR Primer, Index 1 (100% over 23bp)
GCAGTTAAAAAGCTCGTAGTTGGATCTTTGGAATTCTCGGGTGCCAAGGA8911671.2664816765632212RNA PCR Primer, Index 1 (100% over 22bp)
AACTTCTTAGAGGGACAAGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC8488981.2064111017027845RNA PCR Primer, Index 1 (100% over 27bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGTGGAATTCTCGGGTGCCAAGGA8126541.1549029535270134RNA PCR Primer, Index 1 (100% over 22bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC8028141.1409188409001074No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTGGAATTCTCGGGTGCCA7944981.1291005603507829No Hit
CTGGGTCGGGGTTTCGTACGTAGTGGAATTCTCGGGTGCCAAGGAACTCC7107541.010087803457731RNA PCR Primer, Index 1 (100% over 27bp)
GAAGCTACCATCTGTGGGATTATGACTGAACTGGAATTCTCGGGTGCCAA6689240.9506411132968077No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGTGGAATTCTCGGGTGCCAAGGAA6626360.9417049242522969RNA PCR Primer, Index 1 (100% over 23bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCCA6336710.9005413244313276No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTGGAATTCTCGGGTGCCAA6061120.8613758610283899No Hit
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5873010.8346426148266896RNA PCR Primer, Index 1 (100% over 31bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC5405160.7681541281143109RNA PCR Primer, Index 1 (100% over 27bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT5326680.7570009456045589RNA PCR Primer, Index 1 (100% over 25bp)
TTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGTGGAATTCTCGGGTG5267410.7485777915862994No Hit
ACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA4981950.7080096534716995RNA PCR Primer, Index 1 (100% over 28bp)
CAACTTCTTAGAGGGACAAGTGGCGTGGAATTCTCGGGTGCCAAGGAACT4969080.7061806338628754RNA PCR Primer, Index 1 (100% over 25bp)
AACTTCTTAGAGGGACAAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA4822010.6852797858543481RNA PCR Primer, Index 1 (100% over 28bp)
TGGGTCGGGGTTTCGTACGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA4806460.6830698981373929RNA PCR Primer, Index 1 (100% over 28bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC4535940.6446249576106586RNA PCR Primer, Index 1 (100% over 26bp)
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC4462500.6341880345281383RNA PCR Primer, Index 1 (100% over 27bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAA4339120.6166538900575318No Hit
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3941130.5600935548503937RNA PCR Primer, Index 1 (100% over 30bp)
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCTGGAATTCTCGGGTG3890400.5528840626393882No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGTGGAATTCTCGGGTGCCAA3606000.512466566388452No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCTGGAATTCTCGGGTGC3503480.49789694010277696No Hit
AACTTCTTAGAGGGACAAGTGGCGTTGGAATTCTCGGGTGCCAAGGAACT3347890.4757852725863102RNA PCR Primer, Index 1 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGT3334450.47387524744702547No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA3306880.46995713784210874RNA PCR Primer, Index 1 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC3080910.43784341903822677RNA PCR Primer, Index 1 (100% over 24bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGTGGAATTCTCGGGTGCCAAG2956950.42022684788750225No Hit
CTGGGTCGGGGTTTCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2811940.3996187567083593RNA PCR Primer, Index 1 (100% over 31bp)
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA2662320.37835551553724445RNA PCR Primer, Index 1 (100% over 28bp)
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT2649920.3765932899623091RNA PCR Primer, Index 1 (100% over 25bp)
GACGTCGCTTTTTGATCCTTCGATTGGAATTCTCGGGTGCCAAGGAACTC2444760.34743698359507263RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGAGGTAGTGACGAAAAATAACATGGAATTCTCGGGTGCCAAGGAAC2412640.3428722508961272RNA PCR Primer, Index 1 (100% over 24bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAA2391920.3399276288063966No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG2272900.3230131055863318No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGGAATTCTCGGGTG2269860.3225810760905412No Hit
TAGCTCAGCGGTTACTTCGACTGGAATTCTCGGGTGCCAAGGAACTCCAG2267340.32222294637692533RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC2214010.3146439552550462No Hit
TGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGTGGAATTCTCGGGTGC2133100.3031454333785931No Hit
ATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAGGAAC2096170.29789712769453175RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA2091790.2972746641446755RNA PCR Primer, Index 1 (100% over 23bp)
CAACTTCTTAGAGGGACAAGTGGCTGGAATTCTCGGGTGCCAAGGAACTC2085280.2963494957178345RNA PCR Primer, Index 1 (100% over 26bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACATGGAATTCTCGGGTGCCAAG2071910.29444941862615015No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGCC2007710.2853256378268882No Hit
GCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1897680.2696887281486516RNA PCR Primer, Index 1 (100% over 29bp)
GCAGTTAAAAAGCTCGTAGTTGGATCTGGAATTCTCGGGTGCCAAGGAAC1863760.26486818851140914RNA PCR Primer, Index 1 (100% over 24bp)
AACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA1835810.2608960752195186RNA PCR Primer, Index 1 (100% over 23bp)
ATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAG1741430.24748326475480922No Hit
TCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAGGAACT1719640.2443865796517575RNA PCR Primer, Index 1 (100% over 25bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGGT1690560.24025387644860272No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCATGGAATTCTCGGGTGCCAAG1625440.23099934988087784No Hit
TTGATCATCGACACTTCGAACGCTGGAATTCTCGGGTGCCAAGGAACTCC1561990.2219821553059063RNA PCR Primer, Index 1 (100% over 27bp)
CTGCGATCTATTGAAAGTCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA1537700.21853018278855316RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCTGGAATTCTCGGGTG1535890.21827295470059888No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAG1510850.21471439595895525No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGTGGAATTCTCGGGTGCCAAGGAA1510580.2146760249182107RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAG1463610.20800088497831454No Hit
AGTTAAAAAGCTCGTAGTTGGATCTTGTGGAATTCTCGGGTGCCAAGGAA1435160.2039577142035637RNA PCR Primer, Index 1 (100% over 23bp)
ATCTATTGAAAGTCAGCCCTCGTGGAATTCTCGGGTGCCAAGGAACTCCA1421180.20197094698278986RNA PCR Primer, Index 1 (100% over 28bp)
GGGGAGGTAGTGACGAAAAATAACATGGAATTCTCGGGTGCCAAGGAACT1406130.19983211674869497RNA PCR Primer, Index 1 (100% over 25bp)
GACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC1387400.1971703034407483RNA PCR Primer, Index 1 (100% over 27bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCATGGAATTCTCGGGTGCCAAGG1360520.19335025316217877Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCTGGAATTCTCGGGTGCCAAGGAACTC1329710.18897169106832737RNA PCR Primer, Index 1 (100% over 26bp)
ATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGTGCCAAGG1327130.18860503445676824Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG1276080.18135006545673207Illumina Small RNA Adapter 2 (100% over 21bp)
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA1270450.18054995819972514RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCC1257850.17875930963164569RNA PCR Primer, Index 1 (100% over 27bp)
GAAGCTACCATCTGTGGGATTATGACTGAATGGAATTCTCGGGTGCCAAG1241050.17637177820753974No Hit
AATGTAGGTAAGGGAAGTCGGCAAGCCGTGGAATTCTCGGGTGCCAAGGA1217320.1729993900709901RNA PCR Primer, Index 1 (100% over 22bp)
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGTGGAATTCTCGGGTGCCAAG1206690.17148870798538024No Hit
GAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGC1174930.16697513667409428No Hit
GACCCTGTTGAGCTTGACTCTATGGAATTCTCGGGTGCCAAGGAACTCCA1138540.16180357307152193RNA PCR Primer, Index 1 (100% over 28bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAA1136490.16151223739179474No Hit
TGCGATCTATTGAAAGTCAGCCCTCGTGGAATTCTCGGGTGCCAAGGAAC1115920.15858893254692216RNA PCR Primer, Index 1 (100% over 24bp)
TGGGAAATGTGGCGTACGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA1056350.15012314403894655RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGGAA1023940.14551719799994217RNA PCR Primer, Index 1 (100% over 23bp)
GCGATCTATTGAAAGTCAGCCCTCGTGGAATTCTCGGGTGCCAAGGAACT1016800.14450249714469718RNA PCR Primer, Index 1 (100% over 25bp)
GATCTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAGGAA1012620.1439084565879851RNA PCR Primer, Index 1 (100% over 23bp)
TGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1006030.1429719189638864RNA PCR Primer, Index 1 (100% over 30bp)
CTATTGAAAGTCAGCCCTCGACACTGGAATTCTCGGGTGCCAAGGAACTC1001760.1423650880602595RNA PCR Primer, Index 1 (100% over 26bp)
AGTTAAAAAGCTCGTAGTTGGATCTTGGAATTCTCGGGTGCCAAGGAACT989190.14057870294115163RNA PCR Primer, Index 1 (100% over 25bp)
CCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG987050.1402745769145096RNA PCR Primer, Index 1 (100% over 29bp)
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC981870.1395384213920769RNA PCR Primer, Index 1 (100% over 26bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA959670.13638346915307978RNA PCR Primer, Index 1 (100% over 22bp)
TGGAACAATGTAGGTAAGGGAAGTCGGTGGAATTCTCGGGTGCCAAGGAA940870.1337117077975327RNA PCR Primer, Index 1 (100% over 23bp)
ACGTACGCAGTTTTATCCGGTAAAGTGGAATTCTCGGGTGCCAAGGAACT939010.13344737396129236RNA PCR Primer, Index 1 (100% over 25bp)
TCTATTGAAAGTCAGCCCTCGTGGAATTCTCGGGTGCCAAGGAACTCCAG933210.13262310716011294RNA PCR Primer, Index 1 (100% over 29bp)
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATTGGAATTCTCGGGTGCC926940.13173204632504482No Hit
CGCCGCGACCGGCTCCGGGACGGTGGAATTCTCGGGTGCCAAGGAACTCC918080.13047290772876038RNA PCR Primer, Index 1 (100% over 27bp)
CAACTTCTTAGAGGGACAAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC900800.12801716112110859RNA PCR Primer, Index 1 (100% over 27bp)
AGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC899620.1278494654615583RNA PCR Primer, Index 1 (100% over 26bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACTGGAATTCTCGGGTGCCAAGG854250.12140170946681507Illumina Small RNA Adapter 2 (100% over 21bp)
CTGCGATCTATTGAAAGTCAGCCCTCTGGAATTCTCGGGTGCCAAGGAAC853480.1212922809432102RNA PCR Primer, Index 1 (100% over 24bp)
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTTGGAATTCTCGGGTGCCA852000.12108195079394374No Hit
AACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAAC831470.11816433054769998RNA PCR Primer, Index 1 (100% over 24bp)
CACAGGGATAACTGGCTTGTGGCGGCCAAGTGGAATTCTCGGGTGCCAAG826080.117398330882466No Hit
AGTTAAAAAGCTCGTAGTTGGATCTTTGGAATTCTCGGGTGCCAAGGAAC824340.11715105084211218RNA PCR Primer, Index 1 (100% over 24bp)
GCAGTTAAAAAGCTCGTAGTTGGATCTTGGTGGAATTCTCGGGTGCCAAG820940.11666785995866216No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGTGGAATTCTCGGGTGCCAAGG799140.11356975370595329Illumina Small RNA Adapter 2 (100% over 21bp)
ACGAGACTCTGGCATGCTAACTAGTTACTGGAATTCTCGGGTGCCAAGGA796580.11320593939370856RNA PCR Primer, Index 1 (100% over 22bp)
GATCTATTGAAAGTCAGCCCTCGTGGAATTCTCGGGTGCCAAGGAACTCC787390.11189990285873633RNA PCR Primer, Index 1 (100% over 27bp)
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG776660.11037500927655439No Hit
GAAGCTACCATCTGTGGGATTATGACTGAACGTGGAATTCTCGGGTGCCA761550.10822765214451625No Hit
ATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC754590.10723853198310095RNA PCR Primer, Index 1 (100% over 31bp)
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG752950.10700546343931916Illumina Small RNA Adapter 2 (100% over 21bp)
ATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGG728920.10359044081305335Illumina Small RNA Adapter 2 (100% over 21bp)
CAACTTCTTAGAGGGACAAGTGGCGTTGGAATTCTCGGGTGCCAAGGAAC713240.1013620781505545RNA PCR Primer, Index 1 (100% over 24bp)
ACGAGACTCTGGCATGCTAACTAGTTACGCGACTGGAATTCTCGGGTGCC710780.10101247533488182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)