FastQCFastQC Report
Wed 30 Aug 2023
SRR10803859.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10803859.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137669146
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC71215815.172968095552798RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC37312772.7103218901350634RNA PCR Primer, Index 1 (100% over 27bp)
ACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT28996602.106252623953954RNA PCR Primer, Index 1 (100% over 30bp)
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG28271842.05360756723224RNA PCR Primer, Index 1 (100% over 29bp)
CGGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGGAA22394811.6267123499117224RNA PCR Primer, Index 1 (100% over 23bp)
CTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC20342931.4776680607868373RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA19498241.4163115386798435RNA PCR Primer, Index 1 (100% over 28bp)
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT17890511.2995293803885442RNA PCR Primer, Index 1 (100% over 25bp)
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA16711941.2139205105550666RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGAGGTAGTGACGAAAAATAACATGGAATTCTCGGGTGCCAAGGAAC14916141.083477339214409RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA14320971.0402454301561515RNA PCR Primer, Index 1 (100% over 22bp)
GGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTC11416700.8292853069634063RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAGGAACTC10521110.7642315148813373RNA PCR Primer, Index 1 (100% over 26bp)
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA10475180.7608952553537305RNA PCR Primer, Index 1 (100% over 23bp)
GGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGGAAC9769060.7096041694048135RNA PCR Primer, Index 1 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC9475330.6882682340457026RNA PCR Primer, Index 1 (100% over 24bp)
CGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCC7777750.5649595589123506RNA PCR Primer, Index 1 (100% over 27bp)
GCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG7715490.560437122200206RNA PCR Primer, Index 1 (100% over 29bp)
GTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA7332450.5326138944742201RNA PCR Primer, Index 1 (100% over 32bp)
GGGGAGGTAGTGACGAAAAATAACATGGAATTCTCGGGTGCCAAGGAACT6961000.505632540206213RNA PCR Primer, Index 1 (100% over 25bp)
CGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTC6186370.449365030563929RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACT5895490.42823611326825545RNA PCR Primer, Index 1 (100% over 25bp)
GTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5733480.416468044335802RNA PCR Primer, Index 1 (100% over 31bp)
GGTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT5563990.4041566437842216RNA PCR Primer, Index 1 (100% over 30bp)
CCCCGAGTGTTACAGCCCTCCGGTGGAATTCTCGGGTGCCAAGGAACTCC5491510.3988918475603822RNA PCR Primer, Index 1 (100% over 27bp)
CGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5414190.3932754838182842RNA PCR Primer, Index 1 (100% over 31bp)
CGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC4966570.3607612994127239RNA PCR Primer, Index 1 (100% over 27bp)
CGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT4828980.3507670484133024RNA PCR Primer, Index 1 (100% over 30bp)
GGCCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC4689170.3406115412381508RNA PCR Primer, Index 1 (100% over 27bp)
CGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA4323300.3140355065469789RNA PCR Primer, Index 1 (100% over 28bp)
CCGAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC4259190.3093786896883925RNA PCR Primer, Index 1 (100% over 27bp)
CGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG4248510.3086029167348797RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC3990390.28985361759998135RNA PCR Primer, Index 4 (100% over 50bp)
CGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCC3892130.2827162158759959RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAAC3872920.2813208414905109RNA PCR Primer, Index 1 (100% over 24bp)
GAGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG3834610.2785380828904103RNA PCR Primer, Index 1 (100% over 29bp)
ATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3575300.2597023446342872RNA PCR Primer, Index 1 (100% over 31bp)
CCGTGGCGCAATGAAGGTGAAGGGTGGAATTCTCGGGTGCCAAGGAACTC3516610.25543922528581675RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGAGGTAGTGACGAAAAATAACTGGAATTCTCGGGTGCCAAGGAACT3437090.24966305812632847RNA PCR Primer, Index 1 (100% over 25bp)
TACCTGGTTGATCCTGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3348740.2432454981597692RNA PCR Primer, Index 1 (100% over 29bp)
TAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA3303300.23994483121149018RNA PCR Primer, Index 1 (100% over 28bp)
TCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCA3286170.23870054369335594RNA PCR Primer, Index 1 (100% over 28bp)
CCGAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTC3285330.23863952784307965RNA PCR Primer, Index 1 (100% over 26bp)
GGCCGCCGGTGAAATACCACTATGGAATTCTCGGGTGCCAAGGAACTCCA3168960.23018665344230438RNA PCR Primer, Index 1 (100% over 28bp)
AAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2963320.21524939219133382RNA PCR Primer, Index 1 (100% over 30bp)
GGTCCGGTGCGGAGAGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2933230.21306371726893691RNA PCR Primer, Index 1 (100% over 31bp)
ACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2928190.21269762216727922RNA PCR Primer, Index 1 (100% over 31bp)
CGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCCAG2869800.2084562941939075RNA PCR Primer, Index 1 (100% over 29bp)
GAGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCCA2854010.2073093414845473RNA PCR Primer, Index 1 (100% over 28bp)
GTCCGGTGCGGAGAGCCGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2659870.19320741627902596RNA PCR Primer, Index 1 (100% over 30bp)
GTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2657300.1930207368323473RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGGA2641700.1918875853272163RNA PCR Primer, Index 1 (100% over 22bp)
AGGCCCGGTGGGGAAGGTGGCTCGGGGGTGGAATTCTCGGGTGCCAAGGA2579600.18737677068179096RNA PCR Primer, Index 1 (100% over 22bp)
TTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2565760.1863714619105722RNA PCR Primer, Index 1 (100% over 30bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC2510290.18234223665482752RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGAAATGTGGCGTACGGAAGTGGAATTCTCGGGTGCCAAGGAACTCC2451180.17804860938121894RNA PCR Primer, Index 1 (100% over 27bp)
CGCGCGCTACACTGACTGGCTCAGCGTGGAATTCTCGGGTGCCAAGGAAC2397700.17416393358029547RNA PCR Primer, Index 1 (100% over 24bp)
GAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2394230.17391188000832083RNA PCR Primer, Index 1 (100% over 30bp)
GAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2307160.1675872965755159RNA PCR Primer, Index 1 (100% over 32bp)
AGCGCACGGGGTCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT2247900.163282773614358RNA PCR Primer, Index 1 (100% over 30bp)
AACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCC2245060.16307648193009056RNA PCR Primer, Index 1 (100% over 27bp)
CGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCA2231320.16207843695057134RNA PCR Primer, Index 1 (100% over 28bp)
CGTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAAC2213020.16074916306955228RNA PCR Primer, Index 1 (100% over 24bp)
AGCGCACGGGGTCGGCGGCGATGGAATTCTCGGGTGCCAAGGAACTCCAG2198880.15972206292323482RNA PCR Primer, Index 1 (100% over 29bp)
AAGGCCCGGTGGGGAAGGTGGCTCGGGGGTGGAATTCTCGGGTGCCAAGG2145570.1558497355682006Illumina Small RNA Adapter 2 (100% over 21bp)
GAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2141390.1555461090751591RNA PCR Primer, Index 1 (100% over 31bp)
GGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT2081400.15118856043459442RNA PCR Primer, Index 1 (100% over 30bp)
CGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACTC2070940.15042876782282066RNA PCR Primer, Index 1 (100% over 26bp)
CCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2020500.14676491128956373RNA PCR Primer, Index 1 (100% over 30bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG1999110.14521118624502835Illumina Small RNA Adapter 2 (100% over 21bp)
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1877770.13639730139678502RNA PCR Primer, Index 1 (100% over 25bp)
CGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1877430.13637260450500652RNA PCR Primer, Index 1 (100% over 31bp)
GTGGGAAATGTGGCGTACGGAAGATGGAATTCTCGGGTGCCAAGGAACTC1827520.13274724606775726RNA PCR Primer, Index 1 (100% over 26bp)
CGCGCTACACTGACTGGCTCAGCGTGGAATTCTCGGGTGCCAAGGAACTC1824390.1325198893875611RNA PCR Primer, Index 1 (100% over 26bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG1801730.13087391418844133No Hit
AACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1775720.12898460196738634RNA PCR Primer, Index 1 (100% over 31bp)
GGTCCGGTGCGGAGAGCCGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1759610.1278144051245876RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGGAGGTAGTGACGAAAAATAACATGGAATTCTCGGGTGCCAAGGAA1733870.12594470514112147RNA PCR Primer, Index 1 (100% over 23bp)
GGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA1733280.12590184877009405RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAGGAACT1724780.12528442647563165RNA PCR Primer, Index 1 (100% over 25bp)
GTCCGGTGCGGAGAGCCGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG1709670.12418686754982848RNA PCR Primer, Index 1 (100% over 29bp)
GGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAG1703760.12375757746038463RNA PCR Primer, Index 1 (100% over 29bp)
GGGGAGGTAGTGACGAAAAATAACTGGAATTCTCGGGTGCCAAGGAACTC1694260.12306751724892664RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGAGGTAGTGACGAAAAATTGGAATTCTCGGGTGCCAAGGAACTCCA1692750.12295783399426331RNA PCR Primer, Index 1 (100% over 28bp)
GGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACTC1670610.12134963051198124RNA PCR Primer, Index 1 (100% over 26bp)
CGTCGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAA1619660.11764872864105658RNA PCR Primer, Index 1 (100% over 23bp)
CGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCCAAGGAACTC1617570.11749691539453583RNA PCR Primer, Index 1 (100% over 26bp)
CGGTTGGCCCCGGATAGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCA1601550.11633325596426669RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACT1565410.11370812164404652RNA PCR Primer, Index 1 (100% over 25bp)
AACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1546870.11236141466294852RNA PCR Primer, Index 1 (100% over 30bp)
GCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1490350.1082559195943585RNA PCR Primer, Index 1 (100% over 30bp)
ACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1483720.10777433020467782RNA PCR Primer, Index 1 (100% over 32bp)
GGGTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1471090.10685691331302367RNA PCR Primer, Index 1 (100% over 29bp)
TTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAACT1442320.10476712044106092RNA PCR Primer, Index 1 (100% over 25bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGTGGAATTCTCGGGTGCCAAGGA1415020.10278410530708167RNA PCR Primer, Index 1 (100% over 22bp)
CGGTTGGCCCCGGATAGCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAG1407490.10223714179210495RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph