FastQCFastQC Report
Tue 25 Jul 2023
SRR10803860.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10803860.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63412093
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC1813354028.596343602788828RNA PCR Primer, Index 5 (100% over 50bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC31357874.945093044003452No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA8900071.403528819021949No Hit
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC7405241.1677961804540973RNA PCR Primer, Index 1 (100% over 27bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA4784380.7544901569484546RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC3467290.5467868723399494RNA PCR Primer, Index 1 (100% over 27bp)
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT3442530.5428822543359355RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA2508820.3956374693388531RNA PCR Primer, Index 1 (100% over 23bp)
CGGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGGAA2342690.36943899643873923RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA2230870.3518051359698851RNA PCR Primer, Index 1 (100% over 23bp)
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2037880.32137087794910035RNA PCR Primer, Index 1 (100% over 29bp)
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2029250.3200099387982668RNA PCR Primer, Index 1 (100% over 30bp)
ACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1953280.3080295741066298RNA PCR Primer, Index 1 (100% over 30bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT1835230.28941325119169303No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC1740980.2745501555988698RNA PCR Primer, Index 1 (100% over 24bp)
ATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAG1727190.27237549153282165No Hit
GGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTC1661760.26205727036954923RNA PCR Primer, Index 1 (100% over 26bp)
AACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA1541610.24310978033795538RNA PCR Primer, Index 1 (100% over 23bp)
CGGGGAGGTAGTGACGAAAAATAACATGGAATTCTCGGGTGCCAAGGAAC1532380.24165422201093412RNA PCR Primer, Index 1 (100% over 24bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATG1405010.2215681478925479RNA PCR Primer, Index 5 (100% over 49bp)
TTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGG1374600.21677253264609955Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA1370030.21605184992080298RNA PCR Primer, Index 1 (100% over 28bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAG1306830.20608529669569495No Hit
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1271670.20054061297109368RNA PCR Primer, Index 1 (100% over 31bp)
GCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1261040.198864276566301RNA PCR Primer, Index 1 (100% over 29bp)
CTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1244110.19619443881153711RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGAGGTGGGATCCCGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA1230870.19410650899032775RNA PCR Primer, Index 1 (100% over 28bp)
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGGAATTCTCGGGTG1219200.19226616601347632No Hit
GCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTC1133720.17878608737926377RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAGGAACTC1087030.17142313848558824RNA PCR Primer, Index 1 (100% over 26bp)
GGCATTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAG975330.15380820185197167No Hit
GGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGGAAC959980.15138752792783547RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCAGTGATCTCGTATGC926400.14609200803386194RNA PCR Primer, Index 5 (98% over 50bp)
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC910060.14351521246901597RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACCGTGATCTCGTATGC893100.14084064375544267RNA PCR Primer, Index 5 (98% over 50bp)
TAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGA763120.1203429762206398RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACTCC730400.11518307714586869RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACT707040.11149923721962623RNA PCR Primer, Index 1 (100% over 25bp)
CGCGGCGTGGGAAATGTGGCGTACTGGAATTCTCGGGTGCCAAGGAACTC694760.10956269808031728RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGAGGTAGTGACGAAAAATAACTGGAATTCTCGGGTGCCAAGGAACT680130.10725556716760636RNA PCR Primer, Index 1 (100% over 25bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC674960.10644026526612203RNA PCR Primer, Index 5 (100% over 50bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG654160.10316013382494724Illumina Small RNA Adapter 2 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGTGGAATTCTCGGGTGCCAAGG651580.10275327136733997Illumina Small RNA Adapter 2 (100% over 21bp)
GCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCC640950.10107693496254729RNA PCR Primer, Index 1 (100% over 27bp)
CCGTGGCGCAATGAAGGTGAAGGGTGGAATTCTCGGGTGCCAAGGAACTC638700.10072211305184328RNA PCR Primer, Index 1 (100% over 26bp)
GGGGAGGTAGTGACGAAAAATAACATGGAATTCTCGGGTGCCAAGGAACT634730.10009604950273444RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)