FastQCFastQC Report
Tue 18 Jul 2023
SRR10803861.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10803861.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46453288
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC1659059535.71457632880583RNA PCR Primer, Index 6 (100% over 50bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC19355064.16656405462623No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA12561162.704041100384541No Hit
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA2792280.6010941572101419RNA PCR Primer, Index 1 (100% over 23bp)
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2380910.5125385311799673RNA PCR Primer, Index 1 (100% over 31bp)
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC2162900.46560751523121463RNA PCR Primer, Index 1 (100% over 27bp)
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1983290.4269428678546931RNA PCR Primer, Index 1 (100% over 30bp)
GCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1585590.34132998292822675RNA PCR Primer, Index 1 (100% over 29bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGTGGAATTCTCGGGTGCCAAGGA1543810.3323360017056274RNA PCR Primer, Index 1 (100% over 22bp)
ATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAG1496330.3221149813980875No Hit
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT1391410.2995288514345852RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGACCGGCTCCGGGACGGCCGGTGGAATTCTCGGGTGCCAAGGAA1346550.28987183856608817RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAG1260300.27130479978080346No Hit
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATG1238600.26663344045743326RNA PCR Primer, Index 6 (100% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCATTATCTCGTATGC1204300.25924967894629974RNA PCR Primer, Index 6 (98% over 50bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT1162720.2502987517266808No Hit
ATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGG1047980.22559867021684235Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC993200.2138061788005189RNA PCR Primer, Index 1 (100% over 24bp)
AGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA984910.2120215903769826RNA PCR Primer, Index 1 (100% over 32bp)
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC969530.20871073754779207RNA PCR Primer, Index 1 (100% over 26bp)
TTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGG923550.198812622262605Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC918980.19782883829450348RNA PCR Primer, Index 1 (100% over 27bp)
CGGGGAGGTAGTGACGAAAAATAACATGGAATTCTCGGGTGCCAAGGAAC837530.1802950955807477RNA PCR Primer, Index 1 (100% over 24bp)
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGGAATTCTCGGGTG801380.17251308454204578No Hit
GCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCC800480.1723193415286341RNA PCR Primer, Index 1 (100% over 27bp)
AACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA792700.1706445408126977RNA PCR Primer, Index 1 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGTGGAATTCTCGGGTGCCAAGG769010.16554479415967283Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC768980.16553833605922577RNA PCR Primer, Index 6 (100% over 50bp)
TTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGA768760.16549097665594736RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGGAA734780.15817610154958245RNA PCR Primer, Index 1 (100% over 23bp)
CTAAACCATTCGTAGACGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT698060.15027138660238648RNA PCR Primer, Index 1 (100% over 30bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAA687500.14799813524502292No Hit
GCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTC666400.14345593793059383RNA PCR Primer, Index 1 (100% over 26bp)
GGCATTAACGCGAACTCGGCCTACAATATGGAATTCTCGGGTGCCAAGGA626450.13485590083526486RNA PCR Primer, Index 1 (100% over 22bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGGAATTCTCGGGTGCCAAGG597720.12867119330713467Illumina Small RNA Adapter 2 (100% over 21bp)
GGCATTAACGCGAACTCGGCCTATGGAATTCTCGGGTGCCAAGGAACTCC581060.12508479485886984RNA PCR Primer, Index 1 (100% over 27bp)
CATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT574120.123590820955451RNA PCR Primer, Index 1 (100% over 30bp)
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGT547190.11779359945414414No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGAATTCTCGGGTGCCAAGGAACTC545290.11738458642583062RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAGATCTCGTATGC537730.11575714511317262RNA PCR Primer, Index 6 (98% over 50bp)
CCGTGGCGCAATGAAGGTGAAGGGTGGAATTCTCGGGTGCCAAGGAACTC537540.11571624381034126RNA PCR Primer, Index 1 (100% over 26bp)
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATATGGAATTCTCGGGTGC529120.11390367028486768No Hit
TAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGA509070.10958750648608555RNA PCR Primer, Index 1 (100% over 22bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC506970.10913543945479166RNA PCR Primer, Index 6 (98% over 50bp)
TGGTATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC504970.10870489942498797RNA PCR Primer, Index 6 (98% over 50bp)
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA504770.1086618454220076RNA PCR Primer, Index 1 (100% over 28bp)
TCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTAT496920.10697197580502806RNA PCR Primer, Index 6 (100% over 48bp)
GCTAACTAGTTACGCGACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT490130.10551029240384448RNA PCR Primer, Index 1 (100% over 30bp)
ACCAGGAGTGGAGCCTGCGGCTTAATTTGTGGAATTCTCGGGTGCCAAGG477690.10283233341846544Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)