FastQCFastQC Report
Tue 18 Jul 2023
SRR10810229.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10810229.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37232550
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA11731923.1509848237630784Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA3542920.9515652298862152No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT3165780.8502721409089629No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT2814140.7558278979011644No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA2583570.6939009012275549No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG2403020.6454083859418708No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG2316510.622173340262754No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG2270910.6099259921762006No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGA2250660.6044872027298694No Hit
TTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAA2230120.5989705244470228No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA1846770.49600954003956216No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC1837750.4935869286417396No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1485870.3990782259071699No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1248180.3352389240060109No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG1229760.33029163997631106No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1157930.3109993809180408No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA1087440.2920670220009105No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1066840.2865342287863711No Hit
TCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAG986020.2648274157961246No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT963890.2588836918234179No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC912580.24510273940409666No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATAGA905150.24310717369613416No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCAT896790.2408618265469327No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG877210.23560298717117145No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA854000.22936919442799378No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC847880.22772547139532479No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA805200.21626238331782272No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATA730880.19630135459429987No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAG697380.18730385106580127No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCATCAATAGAT688610.18494838521669882No Hit
TCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAGATCGGAAGA680930.18288567396001618No Hit
ACCTGCGGAAGGATCATTAACGGGACTGTAGGCACCATCAATAGATCGGA676650.1817361421659274No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT618960.1662416353432682No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA614900.16515119163205313No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT612100.16439916148638758No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACC611240.1641681807987903No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCA587990.1579236447678174No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCA576310.15478660473161254No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA563730.15140784072001515No Hit
GCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGATC563660.1513890399663735No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA557450.14972114453616525No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA538720.14469060002605247No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC528820.142031636296735No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA521300.14001189819123322No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCAT517010.13885968057519563No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA504960.13562326512688494No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG500200.1343448138792535No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT487560.13094993493596327No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCAC465240.1249551803462293No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATC458000.1230106452552941No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA448760.12052894577459776No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA439320.11799352985492532No Hit
GGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT420640.11297641445455657No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACC419650.11271051808162483No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGA417040.11200951855298658No Hit
TCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA406860.10927535180910253No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA405680.10895842481914346No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG399430.10727978610113999No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG399210.10722069801826627No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGATCG399200.10721801219631746No Hit
GGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGATC391910.10526004799563822No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT390730.10494312100567917No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA385090.10342831742655284No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC381450.10245067823718762No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA380670.1022411841251808No Hit
GCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGATCG378310.10160733014526269No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT377900.10149721144536165No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)