FastQCFastQC Report
Thu 24 Aug 2023
SRR10810230.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10810230.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59558550
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA13832922.3225750123198097Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC7480321.2559607310789132No Hit
TCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAG7021761.1789675873573147No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA4812750.808070377804698No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT4309950.7236492493521082No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA3728310.6259907267722267No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA3385750.5684742157087437No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGA3192250.5359851776109392No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA3013530.5059777311569875No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG2971030.49884189591586764No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA2926110.49129973782101816No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2787300.4679932604134923No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCAT2720370.456755579173771No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA2711940.4553401652659442No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG2639630.4431991712357No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATC2622480.440319651838401No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT2427170.40752671111032757No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC2302090.3865255282406976No Hit
TCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATAGATCGGAAGAGC2247040.37728252282837643No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2124370.3566859837924194No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA2117210.3554838054317978No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA2115770.3552420265436281No Hit
TTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAA1971040.33094156926251567No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1954530.32816950714884763No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1946490.32681957502323344No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC1896330.3183976104186552No Hit
TCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAGATCGGAAGA1806430.30330322010861577No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCA1750340.2938855966103943No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG1725020.2896343178267436No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA1722990.2892934767552266No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1699970.2854283725846247No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1667680.28000681682143036No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1657390.278279105183051No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1480040.2485016844768719No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG1463280.2456876468617856No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1459980.24513357024306334No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1418330.23814045170676587No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCAT1389560.2333099110035419No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG1333670.22392586790645508No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGTAGGCACCATCAA1280250.21495654276338158No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1233180.2070533953563342No Hit
TGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCA1228710.20630287339097408No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC1149680.1930335778826046No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA1092800.1834833117999011No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC1030790.17307170842809302No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATAGA1020660.17137086111062141No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1013590.17018379393051042No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1008460.16932245664140583No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1008010.1692469007388528No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA977910.16419305036808315No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT945560.1587614204845484No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCA939420.15773050216971368No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCATCAATAGAT920550.15456219132265644No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA919080.15431537537431653No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA915240.15367063167253064No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCAT906260.15216287166158343No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA906130.1521410444008459No Hit
TGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAACTGTAGGCACCAT886310.14881322664839894No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC880270.14779909853413153No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCTGTAGGCACCATCA870330.14613015259773784No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA863050.14490782599643545No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA854870.14353438759002696No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACCAT845660.14198801011777484No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC829660.13930157802700033No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAAT828400.13909002149985183No Hit
GCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGATC822860.13815984438842113No Hit
CCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC809790.1359653651742697No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA806750.13545494307702252No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG798430.13405799838981977No Hit
ACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCA774720.13007704183530325No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCCTGTAGGCACCAT757020.1271051763348839No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATA755230.1268046317447285No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC726270.1219421896604266No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG709370.11910464576454598No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG696920.11701426579391204No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAG694240.11656428841870731No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA684770.11497425642498012No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA681020.11434462390370484No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGATCG677010.11367133686095447No Hit
GCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGATCG621110.10428561474381093No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT618050.1037718346064503No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGAT609500.10233627245794265No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGCTGTAGGCACC602740.10120125489959039No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)