FastQCFastQC Report
Tue 18 Jul 2023
SRR10810231.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10810231.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48265047
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA722573014.970937457079447Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAG6097801.2633987489953133No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA4424810.9167731671327286No Hit
TCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATAGATCGGAAGAGC3365580.6973120734762778No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG3033430.6284941564441033No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG2905300.601946994892598No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA2898570.6005526110852021No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2878390.5963715315557447No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2838050.5880135162822901No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT2138630.44310119494962885No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC1988850.412068385637333No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1913120.39637794199185183No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1819490.37697881035938907No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATCA1742020.3609278573788605No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG1666250.3452291261624587No Hit
TTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAA1661450.34423461765198327No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1653100.34250458722230187No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1619600.33556374657627497No Hit
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1594660.33039644610726265Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1559880.3231904031917756No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1539030.31887050684939766No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGA1496650.31008982545899105No Hit
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1427770.2958186283336676Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1281110.2654322495531808No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1270660.2632671216501664No Hit
TCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAGATCGGAAGA1220390.2528517168956657No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1018220.21096426157007575No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC994270.20600207848134902Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT976570.20233482834897065No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA958720.19863649982563986No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA935920.19391258440088124No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG931960.19309211487973896No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT882130.18276787340536518No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA880440.1824177235339686No Hit
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG866240.17947563585714524Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGATC852870.17670551527692493No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGA828790.17171639758270618No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCAT824070.17073846421407193No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG818560.16959685131975527No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATA780530.16171744326696708No Hit
CTGTAGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC777180.16102335920236438Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA767910.15910271464150857No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT756810.156802913711034No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT749850.1553608763708445No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCTGTAGGCACCATC741830.15369921840125836No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC721490.14948498858811843No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC713240.1477756770857387No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT707380.1465615479458665No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCCTGTAGGCACCATC702480.14554632050808944No Hit
GCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGATC699030.14483151751618514No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG646020.13384841415362136No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGATCG640010.13260320662279682No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG635090.13158383539955942Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAGC630660.13066598692009976No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG629930.13051473875079828No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAG627200.12994911203546533No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA623020.12908306087425958No Hit
CGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATAGATCGGAAGAGC616430.12771768356508595No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCATCAATAGAT614730.1273654618009592No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG610990.12659057391988035No Hit
CTGTAGGCACATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC588550.1219412466334074Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGATCG585890.12139012316718556No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA579630.1200931183181071No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG579210.12000609882344049No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC578530.1198652101177898No Hit
GGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGAT572110.11853505498502881No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA570970.11829885921379088No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA556210.11524074554407872No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG552660.11450522362487288No Hit
CTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG552200.11440991655928565No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA547100.11335325126690543No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATAGA541000.11208939670150947No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGA521020.10794975502665521No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT508870.1054324053595141No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG498350.10325277420738864No Hit
GGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT497240.10302279411434118No Hit
CTGAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC490040.10153103134862793Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)