FastQCFastQC Report
Tue 18 Jul 2023
SRR10811655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44874467
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length10-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA23505155.2379786483035No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC18064744.02561661623747No Hit
AGGCTTGTAGCTC16036763.5736936998048354No Hit
NNNNNNNNNN9020132.0100806991200586No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC6820481.5199021751055004No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC6623881.4760910697836254No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCC6527191.454544295757318No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG5609721.2500917281089934No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG4398810.9802478545316203No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG4204030.9368423250575878No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC3402480.7582218191026091No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC3290560.733281132899027No Hit
GGGGCTATAGCTC3161800.7045877558835406No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT2920750.6508712404316691No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT2869860.6395307157631532No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTA2764370.616022915659366No Hit
GGGGCTGTAGCTC2380800.5305466915072217No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGC2314480.5157676858869432No Hit
TCCTCTGTAGTTCAGTCGGTAGAACG2302240.5130400768882669No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT2221460.4950387488724936No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTG2168130.483154485155222No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC2156330.4805249274604197TruSeq Adapter, Index 3 (100% over 50bp)
TGTGGGCACTCGAAGATA2070940.46149628919269386No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATT1837770.40953578345565644No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCAC1806370.4025384858610131No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT1802280.40162705442272995No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA1779550.396561813202149No Hit
GCCGAAGTGGCGAAATCGGTAGACGCAGTTGAT1665970.37125120617031504No Hit
AGGGGCGTAGTTC1654710.36874198416663084No Hit
GCATCCGTAGCTCAGC1544470.34417567566874946No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT1508830.3362335200549569No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGG1492400.3325721952307534No Hit
CCTCTGTAGTTCAGTCGGTAGAACG1461510.3256885480110549No Hit
GCGAAGGTGGCGGAATTGGTAGACG1449570.32302779217411093No Hit
TCCTCTGTAGTTCAG1395090.3108872580035324No Hit
CCTCTGTAGTTCAGTCGGTAGAACGG1371680.3056704829496916No Hit
GATTGTCTGATAGAAAGTGAAAAGCA1364590.30409051989408586No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATT1359320.3029161326863225No Hit
CCGACTTAGCTCAGTAGGTAGAGCAACTGA1356220.3022253166817558No Hit
TCCTCTGTAGTTC1275940.2843354106022028No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTG1136180.25319075098986693No Hit
TGTGGGCACTCGAAGATACGGATTCT1124870.25067038679256065No Hit
GCGGGGTGGAGCAGCCTGGTAGCTCGTCGGG1115020.2484753746490181No Hit
CGGACGTGGGGGTTCAAGTCCCCCCC1111980.24779792927679786No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGC1092890.24354383975190166No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCT1083550.24146247798330395No Hit
AAGGTGGCGGAATTGGTAGACGCG1056160.23535878431714857No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTT1020940.22751022312978114No Hit
GCGGGCGTAGTTCAATGGT1016930.22661661920129325No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG996520.22206837576477512No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGA950470.21180641543887307No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTA940010.20947546853314156No Hit
AGGTGGCGGAATTGGTAGACGCG931150.20750107182331548No Hit
TGTGGGCACTCGAAGATACGGATTCTT903830.20141297722823093No Hit
CGGGCATCGTATAATGGCTATTACCTCAGCC892930.19898397901862544No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCC890420.19842464089879888No Hit
TCCCGGTTTAAGCGTGTAGGCT882590.19667977337758688No Hit
GCCCGGATGGTGGAATCGGTAGACAC872110.19434436959440654No Hit
TGTGGGCACTCGAAGATACGG851130.18966910515059712No Hit
GAAGGTGGCGGAATTGGTAGACGCG815910.1818205439632297No Hit
CCTCTGTAGTTCAGTCGGTAGAACGGC815520.18173363485297775No Hit
GCCGACTTAGCTCAG802190.17876312603334096No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC796610.1775196572251209No Hit
TGTGGGCACTCGAAGATACGGATTC776170.17296472847242955No Hit
GTGAGGTGTCCGAGTGGCTGAAGGAGC773120.17228505466148492No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACG748180.1667273284828096No Hit
TGTGGGCACTCGAAGATACG747580.1665936221593451No Hit
CGGCGAGTAGCGCAGC744640.16593846117436895No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACC737290.16430055871192853No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGC736130.16404205981989714No Hit
GCCGAAGTGGCGAAATCGGTAGACGCAGTTGATT731170.16293675421259043No Hit
CGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAC729740.16261808747499998No Hit
GTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT717870.15997293070912688No Hit
TGAGGTGTCCGAGTGGCTGAAGGAGC707020.15755507469314342No Hit
CCTCTGTAGTTCAGTCGGTAGAACGGCGGA682680.15213105483793266No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGC680890.15173216430626352No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG678730.15125082154179123No Hit
CCCGGTTTAAGCGTGTAGGCT657600.14654213051711568No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACT649060.14463904384647064No Hit
GCGAAGGTGGCGGAATTGGTAGACGC630560.14051643220631457No Hit
TTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACC619310.1380094386413548No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACC617720.1376551168841738No Hit
TAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGC606270.13510355454472586No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACCA605880.1350166454344739No Hit
TCCCCTTCGTCTAGAGGCCCAGGACACCGCC602990.1343726266431198No Hit
AGGGAGTATGCGG569360.12687838721293335No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAG562320.12530956635094964No Hit
GCGGGCATCGTATAATGGCTATTACC558700.1245028715327137No Hit
TTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCA545220.12149893613221077No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATTG537460.11976966768206963No Hit
CGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACG537050.11967830169436887No Hit
TGGCTATAGCTCAGTTGGTAGAGCCCTGGA534670.11914793327795961No Hit
GCCGAGGTGGTGGAATTGGTAGACACGC534580.11912787732943993No Hit
GCCGAGGTGGTGGAATTGGTAGACACG534370.11908108011622735No Hit
TAGCTCAGTAGGTAGAGCAACTGA532850.11874235743011721No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGG527560.11756351334490502No Hit
GAGGTGTCCGAGTGGCTGAAGGAGC519760.11582533113986625No Hit
TGTGGGCACTCGAAGATACGGA518920.11563814228701592No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAC506520.11287487826874913No Hit
TGTGGGCACTCGAAGATACGGATTCTTA499920.1114041087106394No Hit
GATTGTCTGATAGAAAGTG483950.10784529206775871No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCT481480.10729486770282975No Hit
TTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGC479640.10688483497753856No Hit
AGGGGCGTAGTTCAATTGGTAGAGC477160.1063321821738852No Hit
AGGGAGTATGCGGT470560.10486141261577546No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGCT460400.10259731887177624No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCC454730.10133379411503651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)