FastQCFastQC Report
Tue 18 Jul 2023
SRR10811656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23892938
Total Bases592.3 Mbp
Sequences flagged as poor quality0
Sequence length10-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNN8842303.7008006298764933No Hit
TGTGGGCACTCGAAGATACGG7861753.290407399876901No Hit
TGTGGGCACTCGAAGATACG7609413.1847946033258863No Hit
AGGCTTGTAGCTC7521793.148122679596791No Hit
TGTGGGCACTCGAAGATACGGATTCT4135201.7307206003715407No Hit
TGTGGGCACTCGAAGATACGGATTCTT2762521.1562077464060718No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA2628571.1001451558615352No Hit
TGTGGGCACTCGAAGATACGGATTC2320290.9711195835355201No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG2069120.8659964714259921No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGA1994710.8348533780148761No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT1935670.8101431477367915No Hit
TGTGGGCACTCGAAGATACGGA1849010.7738730163699417No Hit
TGTGGGCACTCGAAGATACGGAT1675640.7013118269507082No Hit
GATTGTCTGATAGAAAGTG1587270.6643260029386089No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC1482000.6202669592161499TruSeq Adapter, Index 4 (100% over 50bp)
TGTGGGCACTCGAAGATA1418770.5938030726903489No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC1262540.5284155510720364No Hit
GGGGCTGATTCTGGATTCGACGG1220040.5106278683684694No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG1177150.49267695751774016No Hit
GATTGTCTGATAGAAAGTGAAAAGCA1145210.47930899079887124No Hit
TGTGGGCACTCGAAGATACGGATTCTTA1092610.4572941176175153No Hit
TCCTCTGTAGTTCAG928440.38858343833646575No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT924910.387106014337793No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGA917600.38404653291277946No Hit
CTTTAACAATTTATCAGACAATCTGAAA885230.37049859669832147No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC849980.35574528339712763No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGC809990.3390081203073477No Hit
TGTGGGCACTCGAAGATACGGATT779950.32643536763875586No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACG734840.3075553119503344No Hit
TGTGGGCACTCGAAGA695590.29112786380645195No Hit
AGGTGTAGCGGTGAAATGCGTAGAGATC657590.27522358280090964No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC653720.2736038573406084No Hit
GGGGCTATAGCTC637010.26661015903527646No Hit
TTAACAATTTATCAGACAATCTGAAA633900.265308519195086No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC618060.2586789452180389No Hit
AGTCTGCAACTCGAC567150.23737139400771892No Hit
ATTGAACTCGCTGTGAAG532440.22284408891028806No Hit
TGTGGGCACTCGAAGATACGGATTCTTAA525410.21990179692426273No Hit
ATTTATCAGACAATCTGAAA511850.21422647980754816No Hit
TGTGGGCACTCGAAG493550.2065673129022475No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTA491980.2059102149764922No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC486340.20354968484830122No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT456630.19111504830423115No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTG443570.18564899804285265No Hit
TGTGGGCACTCGAAGATACGGAA440900.18453151303535797No Hit
GATTGTCTGATAGAAAG392550.16429540812435875No Hit
GATTGTCTGATAGAAAGTGAAAAG392010.1640693999205958No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC389510.1630230656439154No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAA367990.15401622019025035No Hit
GCGAAGGTGGCGGAATTGGTAGACG367490.1538069533349143No Hit
GGGGCTGATTCTGGATTCGACGGG361540.15131667775641489No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG352800.14765869312514016No Hit
GGTGGCCGTAGCTCAGTTGGTAGAGTCCCAGATTGTG348070.1456790286736608No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTG326470.13663870052314203No Hit
CCTTAAAGAAGCGTACTTTGTAGTGCTCACACA325690.13631224422881774No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC324370.13575977973073047No Hit
TGTGGGCACTCGA315820.13218131650448345No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG312760.13090060334982664No Hit
GAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCA311030.13017654003036377No Hit
GGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGT305770.1279750527122282No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT299370.12529643696392634No Hit
CCGGGGGTAGAGCACTGTTTCGG298670.1250034633664558No Hit
TGTGGGCACTCGAAGATAC291110.12183934851377423No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTG285810.11962111984721176No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTT285270.11939511164344879No Hit
CGGGACACGTGGTATCC275040.11511351178327253No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACG274400.11484565020844234No Hit
TGGCTATAGCTCAGTTGGTAGAGCCCTGGA272560.11407554818080555No Hit
GATTGTCTGATGAAAATGAGCA270420.11317988603996712No Hit
GCTCAGTCGGAAGACT269250.1126902015984807No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGG267290.11186987552556324No Hit
GATTGTCTGATAGAAAGTGA258030.10799425336473899No Hit
TGTGGGCACTCG257010.10756734897985339No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAA255540.10695210442516528No Hit
TGTGGGCACTCGAAGATACGGATTCA254330.10644567863525196No Hit
TTTAAAAATCTGGATCAAGCTGAAAATTGAAAC251430.10523193087430269No Hit
CATTTTGTGGCTTAGCTACC250090.10467109570200198No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA245660.1028169913637243No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC239320.10016348763806275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)