FastQCFastQC Report
Tue 18 Jul 2023
SRR10811659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20294281
Total Bases508.8 Mbp
Sequences flagged as poor quality0
Sequence length10-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNN10816125.329639419105313No Hit
AGGCTTGTAGCTC4603792.268515942989062No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA3271931.6122423849359333No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC2668301.314803909534908No Hit
TGTGGGCACTCGAAGATACGG2587631.2750537947119192No Hit
TGTGGGCACTCGAAGATACGGATTCT2500791.2322634145057911No Hit
TGTGGGCACTCGAAGATACGGATTCTT2186651.0774710372838536No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC1874510.9236641593757375TruSeq Adapter, Index 11 (100% over 50bp)
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG1616530.7965446028859066No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC1557000.7672112158100107No Hit
TGTGGGCACTCGAAGATACGGATTC1543830.7607217028284963No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG1454890.7168965483428558No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG1421070.7002317549461348No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC1416840.6981474238974025No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC1411180.6953584608392877No Hit
TGTGGGCACTCGAAGATACG1371200.6756583295559966No Hit
TGTGGGCACTCGAAGATACGGAT1333130.656899350117405No Hit
TGTGGGCACTCGAAGATA1189310.5860320944605034No Hit
TGTGGGCACTCGAAGATACGGA1087870.5360475692634787No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC1086020.5351359823981938No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGA988870.4872653532293162No Hit
TGTGGGCACTCGAAGATACGGATTCTTA940870.46361336969760103No Hit
TCCTCTGTAGTTCAG933460.45996209473989247No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC919260.45296504961176004No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG917300.45199926028421505No Hit
TGTGGGCACTCGAAGATACGGATTCTTAA902010.44446511803004995No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGA821280.40468543822764647No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT791020.38977483360952775No Hit
GGGGCTATAGCTC766600.3777418869877676No Hit
TGTGGGCACTCGAAGATACGGATT757590.3733022125790019No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT629490.3101809815287371No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACG585270.2883915917001445No Hit
AGGTGTAGCGGTGAAATGCGTAGAGATC557470.2746931512380261No Hit
ACGGTCCTAAGGTAGCGAAA501170.24695134555395187No Hit
GCGAAGGTGGCGGAATTGGTAGACG493940.24338876553448727No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGC485580.23926937840271356No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC453050.2232402320634074No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTG422970.2084183223835326No Hit
AGGGGCGTAGTTC390070.19220685867116946No Hit
GGGGCTGATTCTGGATTCGACGG317670.15653178351083244No Hit
TGGCTATAGCTCAGTTGGTAGAGCCCTGGA310590.15304311593990447No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGC300900.14826837176443944No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT299290.14747504481681317No Hit
TGTTCGGTCTCTTTTT297850.1467654853108617No Hit
CCGGGGGTAGAGCACTGTTTCGG287230.14153248395446974No Hit
TGTGGGCACTCGAAGATACGGAA273850.13493949354500412No Hit
CCTTAAAGAAGCGTACTTTGTAGTGCTCACACA272240.13414616659737785No Hit
GCCCGGATGGTGGAATCGGTAGACAC269970.1330276248761905No Hit
TCCTCTGTAGTTCAGTCGGTAGAACG260960.1285879504674248No Hit
AGGCTGAGGCGTGATGACGAGG260480.12835143063210763No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAA259020.12763201613301797No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTG258820.1275334662016358No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG257450.12685839917166813No Hit
ATGCGAGAGTAGGGAACTGC253550.12493667550971625No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTG252390.12436508590769982No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC242180.11933411191064124No Hit
TGTGGGCACTCGAAGATACGGATA237640.1170970284682665No Hit
ACGGTCCTAAGGTAGCG236900.11673239372215258No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGG232770.11469733763911125No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGT225450.11109041015052468No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCA222240.10950868375184121No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT219120.10797130482227975No Hit
TTCGGTCTCTTTTT217690.1072666728128974No Hit
TGTGGGCACTCGAAGA214350.10562088895881554No Hit
TGTGGGCACTCGAAGATAC212140.10453191221704282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)