FastQCFastQC Report
Tue 18 Jul 2023
SRR10811660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27091047
Total Bases600.8 Mbp
Sequences flagged as poor quality0
Sequence length10-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNN14296655.277260048310425No Hit
AGGCTTGTAGCTC7273192.6847208969073804No Hit
TGTGGGCACTCGAAGATACGG3339781.2327984222979644No Hit
TGTGGGCACTCGAAGATA2296410.8476638056845864No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC2146110.7921842223373647TruSeq Adapter, Index 12 (100% over 50bp)
TGTGGGCACTCGAAGATACGGATTCTT1854260.6844549049728494No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC1663710.6141180147079587No Hit
TGTGGGCACTCGAAGATACG1644700.6071009363351664No Hit
TGTGGGCACTCGAAGATACGGAT1572740.5805386554458378No Hit
TGTGGGCACTCGAAGATACGGATTCT1502840.5547367733701839No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG1375790.5078393610996282No Hit
TGTGGGCACTCGAAGATACGGATTC1351080.4987182665919114No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA1298500.47930964056132636No Hit
TGTGGGCACTCGAAGATACGGA1298350.4792542717156705No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC1217840.4495359666239551No Hit
TCCTCTGTAGTTCAG1162770.42920821775548207No Hit
GGGGCTATAGCTC1122800.4144542660163707No Hit
TGTTCGGTCTCTTTTT1105680.4081348350988428No Hit
TGTGGGCACTCGAAGAT1059120.3909483454072484No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC1035900.3823772480997135No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG1018220.3758511068250703No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT940130.34702608577660365No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC934240.34485193577051493No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT826100.30493468930898093No Hit
TGTGGGCACTCGAAGATACGGATTCTTA794500.29327031915746926No Hit
TGTGGGCACTCGAAGATACGGATT774360.28583612881406906No Hit
TTCGGTCTCTTTTT676070.2495547698839399No Hit
GCGAAGGTGGCGGAATTGGTAGACG652630.2409024649361097No Hit
TGTGGGCACTCGAAG646540.23865448980247977No Hit
AGGTGTAGCGGTGAAATGCGTAGAGATC636860.23508135362948504No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACG636830.2350702798603539No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGA608880.22475321828646935No Hit
TGTGGGCACTCGAAGA561530.20727511934108708No Hit
TGTGGGCACTCGAAGATACGGATTCTTAA532740.19664799223152946No Hit
CCGGGGGTAGAGCACTGTTTCGG518870.19152821963654634No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC444810.1641907749080351No Hit
AGTCTGCAACTCGAC437240.1613964938306002No Hit
CGGAGCTGCACACTCGCGGGCGTCATT401610.1482445473591331No Hit
TGTGGGCACTCGAAGATAC399900.1476133425186557No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT376300.13890197746879257No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTG366710.1353620626031914No Hit
TGTGGGCACTCGAAGATACGGAA359970.1328741558050525No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC331990.12254602046203678No Hit
CGGGACACGTGGTATCC327970.12106213539845839No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTG319420.11790611119607153No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCA310050.11444740397076571No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG289240.10676589945010247No Hit
AGGGGCGTAGTTC288310.10642261260703582No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)