FastQCFastQC Report
Tue 18 Jul 2023
SRR10811661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45821841
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length10-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA662026914.44784595189006No Hit
AGGCTTGTAGCTC477119710.412495211617534No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCG474267310.350245421173716No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG26234085.725234828517693No Hit
NNNNNNNNNN15467493.3755714878413547No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGC6563461.432386795633113No Hit
GCCGAGGTGGTGGAATTGGTAGACACG6151461.3424733414792305No Hit
AGGGGCGTAGTTC5658351.23485872163015No Hit
GGGGCTATAGCTC5111651.1155488056448888No Hit
CGGTGATTGGCGCAGC4683381.0220846429980845No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTG4619451.0081327810464884No Hit
GCGAAGGTGGCGGAATTGGTAGACG4455110.9722677881929711No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGC4334680.945985561776097No Hit
GCATCCGTAGCTCAGC3980940.868786568396499No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATT3337190.7282967962810573No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA3220950.7029289809634668No Hit
AGGCTTGTAGCTCAGGTGGTTAGA3210800.700713880090501No Hit
CGGCGAGTAGCGCAGC2694440.5880252607048242No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGC2576620.5623126316552842No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAT2565910.5599753183203617No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC2366960.5165571588448399TruSeq Adapter, Index 1 (100% over 50bp)
GCGAAGGTGGCGGAATTGGTAGACGC2176430.47497655102945335No Hit
GCGGGCGTAGTTCAATGGT1891050.412696207470145No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC1620500.3536523117872981No Hit
GCCGAGGTGGTGGAATTGGTAGACAC1527260.33330393687150195No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGC1477680.32248376925754685No Hit
CGCGGGGTGGAGCAGC1365650.2980347297700239No Hit
TCCTCTGTAGTTCAG1281360.2796395718801433No Hit
TCCTCTGTAGTTCAGTCGGTAGAACG1278490.2790132330126151No Hit
GGAGAGATGCCGGAGCGGCTGAACGGACCGGT1277680.2788364614158562No Hit
CCGTGTGAAAGTAGGTCATCGTCAGGCT1268150.2767566671971997No Hit
TAGCTCAGTAGGTAGAGCAACTGA1173490.2560983963957275No Hit
TCCTCTGTAGTTC1159360.25301471409671206No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCA1138430.24844702333107915No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCC948150.2069209746504947No Hit
TTGTAGCTCAGGTGGTTAGA946530.20656743145697704No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT946050.20646267791815698No Hit
GTCCCCTTCGTCTAGA918190.2003826079358095No Hit
GCGGGAATAGCTCAGTTGGTAGAGCA902230.19689955277004256No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACC869100.18966937622606653No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCAC855840.18677555971616241No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGCT801020.17481183263675504No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC776830.16953269075330255No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCT771390.1683454839800086No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGG731740.1596924052003934No Hit
AGGCTTGTAGCTCAGGTGGTTAGAG727610.1587910882934625No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG717290.15653888720883127No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG696680.15204103213574505No Hit
GCGAAGGTGGCGGAATTGG622670.13588934586892745No Hit
GGCTTGTAGCTCAGGTGGTTAGAGCG621990.1357409450222657No Hit
AGGCTTGTAGCTCAGG601100.1311819837182011No Hit
GCCGAAGTGGCGAAATCGGTAGACGCAGTTGATT569470.12427916198303773No Hit
GCCCGGATGGTGGAATCGGTAGACACA564680.12323380896022926No Hit
GGCTTGTAGCTC564050.12309631994052793No Hit
GGGGCTATAGCTCAGC558170.12181308908998223No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGA534020.11654267666809809No Hit
GGTGGCTATAGCTCAGTTGGTAGAGC525700.11472694866188375No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACG511170.11155597174718491No Hit
GCCGACTTAGCTCAG485410.10593419849717518No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC467260.10197320531054176No Hit
AGGCTTGTAGCTCAGGTGG458380.1000352648423707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)