FastQCFastQC Report
Tue 18 Jul 2023
SRR10811662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31442404
Total Bases812.5 Mbp
Sequences flagged as poor quality0
Sequence length10-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA546754017.389064780161213No Hit
AGGCTTGTAGCTC27432138.724565080965183No Hit
NNNNNNNNNN7952272.5291545773662856No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTA5533861.7599990127981309No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC5238521.6660685359809004No Hit
GGGGCTATAGCTC4862781.5465674952843935No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT4513261.4354055116141884No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC3804811.2100887705660166No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG3436211.0928585486020725No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC2660210.846058081309559No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC2579780.8204779761751041No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTG2527140.8037362537546429No Hit
AGGGGCGTAGTTC2453370.7802743072698893No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC2427770.7721324361839508TruSeq Adapter, Index 2 (100% over 50bp)
TCCTCTGTAGTTCAG2242780.7132978763328657No Hit
GCGAAGGTGGCGGAATTGGTAGACG2049470.6518172083788504No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG2041200.6491870023678851No Hit
TGTGGGCACTCGAAGATACGG2004410.6374862431002413No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACT1823730.5800224435765153No Hit
TGTGGGCACTCGAAGATA1794010.5705702401126835No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATT1762220.5604596900415121No Hit
GCGGGCGTAGTTCAATGGT1716930.5460555751398652No Hit
TCCTCTGTAGTTC1710550.5440264682051665No Hit
GCCCGGATGGTGGAATCGGTAGACAC1671740.5316832644221479No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT1521680.4839579060176188No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT1383490.4400077042455151No Hit
CGGCGAGTAGCGCAGC1301910.4140618509958717No Hit
TGTGGGCACTCGAAGATACGGATTCTT1205870.38351711275003014No Hit
TGTGGGCACTCGAAGATACG987740.31414264634472605No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTG927100.29485658920990904No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATTG924610.2940646650300658No Hit
TGTGGGCACTCGAAGATACGGATTCT911240.2898124456386986No Hit
GCCGACTTAGCTCAG888770.2826660455097517No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG884590.2813366306215008No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT854100.27163953494141224No Hit
TGTGGGCACTCGAAGATACGGA810060.2576329723388835No Hit
GCCCGGATGGTGGAATCGG794760.252766932197678No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGT756080.24046507385376767No Hit
TGTGGGCACTCGAAGATACGGATTC754530.23997210900286123No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATA724640.23046583842634932No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCC684190.21760104602688776No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGC645700.20535961563244337No Hit
CGGTGATTGGCGCAGC641880.20414469580633848No Hit
GGGTCTGTAGCTC623970.1984485664645744No Hit
TGTGGGCACTCGAAGATACGGATTCTTA615540.19576747375932196No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTA601460.19128944466205575No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCT599400.19063427847310913No Hit
GCATCCGTAGCTCAGC597410.1900013752129131No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC596640.18975648299665637No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGG585230.18612762561030638No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG580250.18454377725061988No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAT564130.17941694280119294No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC563840.17932471066779754No Hit
TAGCTCAGTAGGTAGAGCAACTGA532670.1694113465369887No Hit
GCCGAAGTGGCGAAATCGGTAGACGCAGTTGATT502150.15970470960172128No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGG494830.15737664333808574No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGTT479440.15248197943134376No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGA472560.15029385157699773No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCG471380.14991856220663027No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT468880.14912345760839407No Hit
GCCGACTTAGCTC463700.14747600088084867No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGC453390.14419698951772264No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG439080.13964581079741867No Hit
CCGTGTGAAAGTAGGTCATCGTCAGGCT433980.13802379741701684No Hit
TGTGGGCACTCGAAGA417550.1327983699974086No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGA417140.13266797284329784No Hit
GCCCTGGATTGTGATTCC413090.13137990339415523No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA409280.13016816398644326No Hit
TGTGGGCACTCGAAGATACGGAT404480.1286415631578298No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGC401240.12761110759851568No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTG395170.1256805936339982No Hit
GCCGAGGTGGTGGAATTGGTAGACACGC391710.12458016887003934No Hit
GGGGCTGATTCTGGATTCGACGG386010.12276733038606082No Hit
GATTGTCTGATAGAAAGTGAAAAGCA377150.11994947968991176No Hit
CGGAGCTGCACACTCGCGGGCGTCATT356410.11335329194294431No Hit
GCGAAGGTGGCGGAATTGGTAGACGC351710.11185849529826027No Hit
GGGGCTGATTCTGGATTCGACGGG345010.10972761497498727No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCAC344230.10947954234033759No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACC341730.10868443774210139No Hit
GCCCGGATGGTGGAATCGGTAGACACA340160.10818511205440907No Hit
GGCTTGTAGCTC333320.10600970587363485No Hit
TTTCTCTGAGATGTTCGC326390.10380567592732413No Hit
GATTGTCTGATAGAAAGTG323450.10287063291979837No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCT321600.1022822555171036No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC314940.10016409686740238No Hit
GCGTTCATAGCTC314640.10006868431561404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)