FastQCFastQC Report
Thu 24 Aug 2023
SRR10811663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110378417
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length10-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNN1944161217.613599223841018No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA39824063.607957160682962No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC29576062.679514782314735TruSeq Adapter, Index 5 (100% over 50bp)
AGGCTTGTAGCTC19541401.770400457908361No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACC17761291.6091270814293341No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACCA6624320.6001463130242211No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC6232190.5646203460229005No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC5768660.5226257231067193No Hit
GGGGCTATAGCTC4873820.4415555262040041No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATT4062380.36804115427747075No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC3904910.3537747782702845No Hit
GTTCAGGGACGTCATTACGGGCAGGGATGCCCACC3739900.3388252977029015No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT3639040.32968764174249754No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG3249870.2944298431096362No Hit
TGTGGGCACTCGAAGATACGGATTCTT3119120.2825842302123249No Hit
TGTGGGCACTCGAAGATACGG2962790.2684211352659642No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCA2951190.26737020517335375No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATTG2940050.26636094989476067No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC2817850.2552899449536407No Hit
GCGAAGGTGGCGGAATTGGTAGACG2781980.2520402154345084No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCAC2531630.2293591508926967No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG2511250.22751277543688633No Hit
GCGGGCGTAGTTCAATGGT2366330.21438339707299842No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG2285120.2070259804505078No Hit
GCATCCGTAGCTCAGC2135300.19345267471991376No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT2115360.1916461621296852No Hit
TGTGGGCACTCGAAGATA2111200.19126927685509387No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT2087200.18909493873245165No Hit
TGTGGGCACTCGAAGATACG2039620.18478431340431345No Hit
GTTCAGGGACGTCATTACGGGCAGGGATGCCCACCC2007970.18191690500507904No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACT1990170.1803042708974527No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG1958670.1774504521114848No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT1904460.1725391658769667No Hit
CGGTGATTGGCGCAGC1895230.1717029516739672No Hit
TGTGGGCACTCGAAGATACGGATTCTTA1826210.1654499176229353No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT1787420.1619356436322148No Hit
TGTGGGCACTCGAAGATACGGA1755560.15904920977440726No Hit
TGTGGGCACTCGAAGATACGGATTC1733180.15702163947504338No Hit
TGTGGGCACTCGAAGATACGGATTCT1709290.1548572670687966No Hit
AGGGGCGTAGTTC1643310.14887964917996604No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACCC1600070.144962216662339No Hit
TCCTCTGTAGTTCAG1528400.13846909944359864No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCC1489000.13489956102559433No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTA1485860.1346150851212153No Hit
TCTGAGAGGATGACC1477690.1338749041852992No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCG1401680.12698859415604774No Hit
TGTGGGCACTCGAAGA1342290.121608013276726No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG1331540.12063409099262584No Hit
TTCAGGGACGTC1281380.11608972431630361No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTG1279790.11594567441567856No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC1245680.11285539635887332No Hit
GTACTAATGAACCGTGAGGCTTAACCTT1237770.11213877075261915No Hit
GGGGCTGATTCTGGATTCGACGG1220010.11052976054186392No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA1114160.10094002344679395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)