FastQCFastQC Report
Tue 18 Jul 2023
SRR10811664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44584192
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length10-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA615442813.80405862239244No Hit
AGGCTTGTAGCTC44302469.936808992747922No Hit
NNNNNNNNNN40192429.014948616765333No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG13471373.0215575063017854No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC8637091.9372539038051872No Hit
GCGAAGGTGGCGGAATTGGTAGACG8541631.9158427273953962No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC8077061.8116421174572368TruSeq Adapter, Index 6 (100% over 50bp)
GGGGCTATAGCTC7975631.788891901416538No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC3635640.8154549486957171No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC3539060.7937925621709148No Hit
GCATCCGTAGCTCAGC3031600.679971950596301No Hit
AGGGGCGTAGTTC2841920.6374277232611953No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTG2682810.6017401862974213No Hit
TCCTCTGTAGTTC2626660.589146036335031No Hit
TCCTCTGTAGTTCAG2418410.542436655575142No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT2392480.5366206928231423No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATT2229030.4999597166637No Hit
GCCCGGATGGTGGAATCGG2161530.4848198213393662No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCG1837300.4120967359910885No Hit
GCGGGCGTAGTTCAATGGT1815170.40713309327216246No Hit
GCCCGGATGGTGGAATCGGTAGACAC1611970.3615564009772791No Hit
TGTGGGCACTCGAAGATA1610640.3612580889657034No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC1538170.3450034487560075No Hit
CGGTGATTGGCGCAGC1479140.3317633299264457No Hit
GCCGACTTAGCTCAG1416380.31768659169599844No Hit
TGTGGGCACTCGAAGATACGG1385360.31072896868917127No Hit
GCGAAGGTGGCGGAATTGGTAGACGC1374400.30827069827799053No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGC1373840.30814509322048494No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT1371490.3076180005684526No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACT1342640.3011470971594596No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG1317520.2955128131513519No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT1317470.29550159841407464No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCC1315910.29515169861102336No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG1287160.28870322467658494No Hit
GCCGAAGTGGCGAAATCGGTAGACGCAGTTGATT1181660.2650401290215151No Hit
GCCGAGGTGGTGGAATTGGTAGACACG1099550.24662328746475878No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG1083450.24301214206147326No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAT1055240.23668478728962947No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGC1011250.22681806143307476No Hit
CGGCGAGTAGCGCAGC905310.2030562760899648No Hit
CACGGCGGGTGCTAACG892520.2001875462944355No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTA877520.1968231251112502No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGG864190.1938332761531262No Hit
CGCGGGGTGGAGC853420.19141762174359916No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG833820.18702144473090374No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATTG825960.18525848803091463No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGC818150.18350674606820283No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA807050.18101707439264572No Hit
GCCGAGGTGGTGGAATTGGTAGACACGC727160.1630981671710009No Hit
GCCGACTTAGCTC716020.16059952370562194No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCAC710050.1592604840747142No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACC690830.1549495390653261No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC651530.14613475556538066No Hit
CAGTTCGACTCTGGGTATCAGCACCA646510.14500879594274133No Hit
TGTGGGCACTCGAAGATACGGATTCTT645240.1447239416158983No Hit
TCCTCTGTAGTTCAGTCGGTAGAACG611100.1370665190029686No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT604900.13567589158058532No Hit
TAGCTCAGTAGGTAGAGCAACTGA603860.1354426250452178No Hit
GGGGCTGTAGCTC596150.1337133125570606No Hit
CGCGGGGTGGAGCAGC593600.13314136095591908No Hit
TGTGGGCACTCGAAGATACGGA580330.13016496968252783No Hit
GGAGAGATGCCGGAGCGGCTGAACGGACCGGT552870.1240058359698433No Hit
TGTGGGCACTCGAAGATACG539340.12097112806261018No Hit
GGCTTGTAGCTC526030.1179857649993971No Hit
TGTGGGCACTCGAAGA516050.11574730343885116No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCA497940.11168532559701878No Hit
GCGAAGGTGGCGGAATTGG485410.10887491243533134No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTA455360.10213485533168348No Hit
TGGGGTATCGCCAAGCGG450300.1009999239192223No Hit
TGTGGGCACTCGAAGATACGGATTC448250.10054011969085365No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT447600.10039432810624897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)