FastQCFastQC Report
Tue 18 Jul 2023
SRR10811665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52192138
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length10-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTTGTAGCTC45052308.632008905249293No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA38519837.380389360558482No Hit
NNNNNNNNNN33592976.436404272229661No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG20716913.9693545414828573No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCG17221233.299583167104594No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC6257781.1989890124830678TruSeq Adapter, Index 9 (100% over 50bp)
GGGGCTATAGCTC6169621.1820975795243338No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATT3996920.7658088273754948No Hit
GCGAAGGTGGCGGAATTGGTAGACG3892360.7457751587030216No Hit
GCCGAGGTGGTGGAATTGGTAGACACG3805930.7292151932921391No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC3558930.6818900578474099No Hit
GCATCCGTAGCTCAGC3098660.5937024461423672No Hit
AGGGGCGTAGTTC2756760.5281944955004526No Hit
TGTGGGCACTCGAAGATACGG2505520.48005697716387863No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGC2473830.4739851814462937No Hit
TGTGGGCACTCGAAGATACG1938860.3714850692646467No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGC1905240.3650434860514815No Hit
CGGTGATTGGCGCAGC1895290.3631370686519874No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA1817620.34825551695161444No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACC1686170.3230697313070409No Hit
GCGAAGGTGGCGGAATTGGTAGACGC1646560.3154804656594064No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTG1546810.2963683917298042No Hit
TGTGGGCACTCGAAGATACGGA1463340.28037556154530396No Hit
GCGGGCGTAGTTCAATGGT1430640.27411025009169004No Hit
TGTGGGCACTCGAAGA1389050.26614161696154315No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC1329120.2546590446246904No Hit
TGTGGGCACTCGAAGATA1300010.2490815762328035No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGC1139150.2182608422747503No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT1133560.217189799735738No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGC1125590.21566274981875624No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAT987630.18922964987561922No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCAC945040.18106941700682966No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCA914080.17513748909845386No Hit
TGTGGGCACTCGAAGATAC841390.1612101040965212No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG816370.15641627863568264No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGCACG806770.15457692114471341No Hit
CACGGCGGGTGCTAACG806700.15456350916300843No Hit
GCCGAAGTGGCGAAATCGGTAGACGCAGTTGATT804670.1541745616935639No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACC764500.1464780001922895No Hit
GGAGAGATGCCGGAGCGGCTGAACGGACCGGT760290.14567136529260405No Hit
TGTGGGCACTCGAAGATACGGATTCTT752970.14426885520573998No Hit
TGTGGGCACTCGAAG724850.13888107055510926No Hit
GTCCCCTTCGTCTAGA719610.13787708792462192No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACCA718700.1377027321624571No Hit
TGTGGGCACTCGAAGATACGGATTCTTA689300.13206969984636382No Hit
CGCGGGGTGGAGCAGC680450.13037404215937656No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG660340.1265209714152733No Hit
TCCTCTGTAGTTCAGTCGGTAGAACG656850.12585228832741052No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC649780.12449767817520715No Hit
GCGGGAATAGCTC597370.114455935872947No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC589220.11289439800300959No Hit
AGGCTTGTAGCTCAGGTGGTTAGA584540.11199771122616206No Hit
GGTGGCTATAGCTCAGTTGGTAGAGC567610.10875392765094237No Hit
TGTGGGCACTCGAAGATACGGATTC565630.10837456016842997No Hit
CGGCGAGTAGCGCAGC537820.10304617143677847No Hit
TGTGGGCACTCGAA532980.10211882870174815No Hit
GGCTTGTAGCTC526990.10097114626727881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)