FastQCFastQC Report
Tue 18 Jul 2023
SRR10811666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76605619
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length10-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNN35196024.594443653017151No Hit
AGGCTTGTAGCTC21563962.8149318916148958No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA13385561.7473339651494755No Hit
GCGAAGGTGGCGGAATTGGTAGACGCG7825651.0215503904485126No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC7772561.0146200894219No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC5217240.6810518690541487TruSeq Adapter, Index 10 (100% over 50bp)
TCCTCTGTAGTTCAGTCGGTAGAACGG5140830.6710774049094231No Hit
GGGGCTATAGCTC4005340.5228519855704057No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC3981730.5197699662214074No Hit
TCCTCTGTAGTTCAGTCGGTAGAACG3969140.5181264836460626No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACC3943370.5147625006463299No Hit
CCTCTGTAGTTCAGTCGGTAGAACG3403060.44423112095732825No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCC3152570.41153247518305414No Hit
TCCCGGTTTAAGCGTGTAGGCT3107040.4055890469340115No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCG2678680.3496714777541318No Hit
CCCGGTTTAAGCGTGTAGGCT2615510.34142534635742583No Hit
GGGGCTGTAGCTC2595010.3387493024499939No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGC2498950.32620975231594956No Hit
CCTCTGTAGTTCAGTCGGTAGAACGG2383670.3111612478452788No Hit
GCATCCGTAGCTCAGC2252760.29407242306860026No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC1969520.2570986339787947No Hit
TGTGGGCACTCGAAGATACGG1900800.24812801264617415No Hit
TGTGGGCACTCGAAGATACG1869720.24407086900505298No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA1761160.22989958478111117No Hit
TCGTGGAGCTGAAATCAGT1732190.2261178778543647No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGC1730590.22590901589085782No Hit
GTCCCGGTTTAAGCGTGTAGGCT1696840.2215033338481346No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGC1692220.22090024492850843No Hit
GCGAAGGTGGCGGAATTGGTAGACG1647670.21508474463211372No Hit
TGTGGGCACTCGAAGATA1597110.20848470658529633No Hit
TCGTGGAGCTGAAATCAGTC1546710.20190555473482957No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACCA1519900.1984058114588174No Hit
TGTGGGCACTCGAAGATACGGA1444120.1885135867122228No Hit
GCGGGGTGGAGCAGCCTGGTAGCTCGTCGGG1412380.18437028751115503No Hit
CCGACTTAGCTCAGTAGGTAGAGCAACTGA1361060.17767104003167183No Hit
AGGGGCGTAGTTC1328050.1733619566470705No Hit
CCTCTGTAGTTCAGTCGGTAGAACGGC1266510.1653286033756871No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT1244770.16249069144653736No Hit
AGGAGGTCTGCGGT1216020.1587377030397731No Hit
CGTGGAGCTGAAATCAGT1183130.1544442843024348No Hit
TGTGGGCACTCGAAGA1168690.1525593050817852No Hit
CGACTTACCAACCCGAT1140540.148884639911336No Hit
GCGAAGGTGGCGGAATTGGTAGACGC1139920.1488037059004771No Hit
TGTGGGCACTCGAAGATAC1104030.14411867098156336No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACC1098660.1434176780165434No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC1098360.14337851639838586No Hit
GAAGGTGGCGGAATTGGTAGACGCG1097290.14323883996029066No Hit
CGCTTACCGGAACGGCCTGGAATGGCC1053590.13753429758200897No Hit
AAGGTGGCGGAATTGGTAGACGCG1022630.13349281858815082No Hit
CCCGGTTTAAGCGTGTAGGCTG1019190.13304376536661103No Hit
ACGATGTCGACTTGGAGGTTGTGCC1018310.13292889128668223No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG982950.12831304189318019No Hit
GCCGAGGTGGTGGAATTGGTAGACACG953780.12450522722099537No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATT935710.12214639242063953No Hit
AGACTGACGCTCAGGTGCG927800.12111383108855239No Hit
TCCCGGTTTAAGCGTGTAGGCTG904100.11802006325410672No Hit
AGACTGACGCTCAGGTGC895520.11690004097480108No Hit
GCTTACCGGAACGGCCTGGAATGGCC891370.1163583052569551No Hit
CGGGCATCGTATAATGGCTATTACCTCAGCC879960.11486885837969667No Hit
CGTGGAGCTGAAATCAGTC866510.11311311249896695No Hit
GCGGACGTAGCTCAGTCGGTTAGAGTGCCG847290.11060415816234054No Hit
CCTCTGTAGTTCAGTCGGTAGAACGGCGGA838500.10945672275032461No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC832190.10863302338174437No Hit
CGATGTCGACTTGGAGGTTGTGCC831000.1084776822963861No Hit
ACGGTCCTAAGGTAG829700.10830798195103677No Hit
GTGAGGTGTCCGAGTGGCTGAAGGAGC797140.10405764099367175No Hit
TAACGGTCCTAAGGTAG791750.10335403725410795No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC787450.10279272072718322No Hit
TGTGGGCACTCGAAGATACGGATTCTT787210.10276139143265718No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG786810.10270917594178046No Hit
CCGATGGTAGTGTGGGGT771340.1006897418321233No Hit
GTTCAGGGACGTCATTACGGGCAGGGATGCCCACC770150.10053440074676506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)