FastQCFastQC Report
Thu 24 Aug 2023
SRR10811667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10811667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75269497
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length10-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNN24639333.2734814210330114No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGA10947671.4544630210561924No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGC8000091.0628595007085007No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC7082730.9409827728754452No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTG6458500.858050107602021No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT5812190.7721839831080577No Hit
TGTGGGCACTCGAAGATACG5753290.7643587680677606No Hit
TGTGGGCACTCGAAGATACGGATTCT5665210.7526568166119139No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAC4534560.6024432447050895No Hit
TGTGGGCACTCGAAGATACGGATTC4339140.5764805363320018No Hit
TGTGGGCACTCGAAGATA4131250.5488611143502128No Hit
TGTGGGCACTCGAAGATACGGATTCTT4067100.5403384056093798No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGA3985970.5295598029570996No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAC3699950.491560346151908No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC3605640.4790307021714254TruSeq Adapter, Index 1 (100% over 50bp)
GCGAAGGTGGCGGAATTGGTAGACGCG3518520.46745629242081954No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGT3241930.4307096671577333No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTT3226120.42860921469954816No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTG3208330.42624570747430396No Hit
CCTTAAAGAAGCGTACTTTGTAGTGCTCACACA2832540.3763197726696646No Hit
TGTGGGCACTCGAAGATACGGATT2668120.35447559852831223No Hit
TGTGGGCACTCGAAGATACGGAT2660620.3534791789561182No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAC2617630.347767701968302No Hit
TGTGGGCACTCGAAGATACGGATTCTTA2553010.33918255093427824No Hit
AGGCTTGTAGCTC2520150.3348169046486387No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATT2445120.3248487232484097No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGC2364090.31408340619042535No Hit
CCTTAAAGAAGCGTACTTTGCAGTGCTCACACA2181660.28984649651637767No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGC2162420.2872903481738426No Hit
TGTGGGCACTCGAAGATACGG2156820.28654635489327107No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGG2118900.281508457536258No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACG2100630.27908117945839334No Hit
ATTGAACTCGCTGTGAAG2033010.27009746059549194No Hit
TGTGGGCACTCGAAGATACGGATTCTTAA1842220.24474987523830535No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCT1700300.22589495981353508No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGT1692020.22479491260583287No Hit
GGCGCGTTAACAAAGCGGTTATGTAGCGGATT1689460.2244548013918573No Hit
TGTGGGCACTCGAAGATACGGA1686810.22410273314301543No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACC1672760.2222361071444386No Hit
TCCTCTGTAGTTCAG1499050.19915770129299523No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCT1480400.19667993795680605No Hit
TGGCTATAGCTCAGTTGGTAGAGCCCTGGA1421800.1888945796993967No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGA1321980.17563289947320893No Hit
TTCAGGGACGTCATTACGGGCAGGGATGCCCACCA1200440.15948558816594724No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC1181910.15702376754291317No Hit
GCGAAGGTGGCGGAATTGGTAGACG1170250.15547466724800885No Hit
CATTTTGTGGCTTAGCTACC1128140.14988010348999675No Hit
TCCTCTGTAGTTCAGTCGGTAGAACG1112160.14775706552150866No Hit
TGTGGGCACTCGAAGA1090270.1448488489301317No Hit
ATCTGAGGGTCGGACGTTCGAATCGTTCCGGGTGC1006560.13372747794501672No Hit
GCCCAGATGGCGGAATTGGTAGACGCACCAGCT1001990.13312032628569312No Hit
CCTTAAAGAAGCGTTCTTTGCAGTGCTCACACA987750.13122845765795405No Hit
GCCCAGATGGCGGAATTGGTAGACGCA951530.1264164154039717No Hit
GTGGCTATAGCTCAGTTGGTAGAGCCCTGGAT935180.1242442207365887No Hit
CGGGACGTAGCGCAGCCTGGTAGCGCATCACACT934850.12420037827541215No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTC931550.12376195366364678No Hit
TGTGGGCACTCGAAGATAC930490.12362112636411002No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCC925080.12290237571270073No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAA887410.1178976923414275No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGT883800.11741808238734477No Hit
ATTTATCAGACAATCTGAAA880160.11693448675497327No Hit
CCGACTTAGCTCAGTAGGTAGAGCAACTGA854930.11358253131411254No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACT821050.1090813719666547No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTGTG809760.10758142837064529No Hit
GATTGTCTGATAGAAAGTG790380.10500668019609591No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCG784960.10428660098525701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)